Gene expression in pancreatic cancer-like cells and induced pancreatic stem cells generated by transient overexpression of reprogramming factors

Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Hirofumi Noguchi

Research output: Contribution to journalArticlepeer-review

Abstract

We previously reported that transient overexpression of reprogramming factors can be used to generate induced pluripotent stem (iPS) cells, induced tissue-specific stem (iTS) cells, and fibroblast-like (iF) cells from pancreatic tissue. iF cells have tumorigenic ability and behave similarly to pancreatic cancer cells. In this study, we analyzed gene expression in iF cells and iTS-P cells (iTS cells from pancreatic tissue) via microarray analysis and quantitative reverse transcriptionpolymerase chain reaction (qRT-PCR). The expression levels of the Mybl2 and Lyn genes, which are reported to be oncogenes, were significantly higher in iF cells than in iTS-P cells. The expression level of Nestin, which is expressed in not only pancreatic progenitor cells but also pancreatic ductal adenocarcinomas, was also higher in iF cells than in iTS-P cells. Itgb6 and Fgf13, which are involved in the pathogenesis of diseases such as cancer, exhibited higher expression levels in iF cells than in iTS-P cells. Unexpectedly, the expression levels of genes related to epithelial-mesenchymal transition (EMT), except Bmp4, were lower in iF cells than in iTS-P cells. These data suggest that the Mybl2, Lyn, Nestin, Itgb6, and Fgf13 genes could be important biomarkers to distinguish iTS-P cells from iF cells.

Original languageEnglish
Article number454
Pages (from-to)1-12
Number of pages12
JournalJournal of Clinical Medicine
Volume10
Issue number3
DOIs
Publication statusPublished - Feb 1 2021

Keywords

  • Epithelial-mesenchymal transition (EMT)
  • Induced fibroblast-like (iF) cells
  • Induced pluripotent stem (iPS) cells
  • Induced tissuespecific stem (iTS) cells
  • Pancreatic cancer
  • Reprogramming factors

ASJC Scopus subject areas

  • Medicine(all)

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