Gene expression and localization of high-mobility group box chromosomal protein-1 (HMGB-1) in human osteoarthritic cartilage

Chuji Terada, Aki Yoshida, Yoshihisa Nasu, Shuji Mori, Yasuko Tomono, Masato Tanaka, Hideo K. Takahashi, Masahiro Nishibori, Toshihumi Ozaki, Keiichiro Nishida

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We investigated the expression and localization of high-mobility group box chromosomal protein-1 (HMGB-1) in human osteoarthritic (OA) cartilage in relation to the histopathological grade of cartilage destruction, and examined the role of HMGB-1 in the regulation of proinflammatory cytokine expression in chondrocytes. An immunohistochemical study demonstrated that total HMGB-1-positive cell ratios increase as the Osteoarthritis Research Society International (OARSI) histological grade increased. The population of cytoplasmic HMGB-1-positive chondrocytes was especially increased in the deep layers of higher-grade cartilage. The ratios and localization of receptors for advanced glyca-tion end products (RAGE) expression by chondrocytes in Grade 2, 3, and 4 were significantly higher than those in Grade 1. In vitro stimulation with IL-1β, but not TNFα, significantly upregulated the expression of HMGB-1 mRNA by human OA chondrocytes. Both IL-1β and TNFα promoted the trans-location of HMGB-1 from nuclei to cytoplasm. IL-1β and TNFα secretions were stimulated at higher levels of HMGB-1. The results of our study suggest the involvement of HMGB-1 in the pathogenesis of cartilage destruction in OA.

Original languageEnglish
Pages (from-to)369-378
Number of pages10
JournalActa Medica Okayama
Volume65
Issue number6
Publication statusPublished - Dec 22 2011

Fingerprint

High Mobility Group Proteins
HMGB1 Protein
Cartilage
Gene expression
Gene Expression
Chondrocytes
Proteins
Interleukin-1
Cytoplasm
Cells
Cytokines
Messenger RNA

Keywords

  • Cartilage
  • Chondrocyte
  • HMGB-1
  • Osteoarthritis
  • RAGE

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Gene expression and localization of high-mobility group box chromosomal protein-1 (HMGB-1) in human osteoarthritic cartilage. / Terada, Chuji; Yoshida, Aki; Nasu, Yoshihisa; Mori, Shuji; Tomono, Yasuko; Tanaka, Masato; Takahashi, Hideo K.; Nishibori, Masahiro; Ozaki, Toshihumi; Nishida, Keiichiro.

In: Acta Medica Okayama, Vol. 65, No. 6, 22.12.2011, p. 369-378.

Research output: Contribution to journalArticle

@article{7d5cc8b9635b46e8beec03d95c17d485,
title = "Gene expression and localization of high-mobility group box chromosomal protein-1 (HMGB-1) in human osteoarthritic cartilage",
abstract = "We investigated the expression and localization of high-mobility group box chromosomal protein-1 (HMGB-1) in human osteoarthritic (OA) cartilage in relation to the histopathological grade of cartilage destruction, and examined the role of HMGB-1 in the regulation of proinflammatory cytokine expression in chondrocytes. An immunohistochemical study demonstrated that total HMGB-1-positive cell ratios increase as the Osteoarthritis Research Society International (OARSI) histological grade increased. The population of cytoplasmic HMGB-1-positive chondrocytes was especially increased in the deep layers of higher-grade cartilage. The ratios and localization of receptors for advanced glyca-tion end products (RAGE) expression by chondrocytes in Grade 2, 3, and 4 were significantly higher than those in Grade 1. In vitro stimulation with IL-1β, but not TNFα, significantly upregulated the expression of HMGB-1 mRNA by human OA chondrocytes. Both IL-1β and TNFα promoted the trans-location of HMGB-1 from nuclei to cytoplasm. IL-1β and TNFα secretions were stimulated at higher levels of HMGB-1. The results of our study suggest the involvement of HMGB-1 in the pathogenesis of cartilage destruction in OA.",
keywords = "Cartilage, Chondrocyte, HMGB-1, Osteoarthritis, RAGE",
author = "Chuji Terada and Aki Yoshida and Yoshihisa Nasu and Shuji Mori and Yasuko Tomono and Masato Tanaka and Takahashi, {Hideo K.} and Masahiro Nishibori and Toshihumi Ozaki and Keiichiro Nishida",
year = "2011",
month = "12",
day = "22",
language = "English",
volume = "65",
pages = "369--378",
journal = "Acta Medica Okayama",
issn = "0386-300X",
publisher = "Okayama University",
number = "6",

}

TY - JOUR

T1 - Gene expression and localization of high-mobility group box chromosomal protein-1 (HMGB-1) in human osteoarthritic cartilage

AU - Terada, Chuji

AU - Yoshida, Aki

AU - Nasu, Yoshihisa

AU - Mori, Shuji

AU - Tomono, Yasuko

AU - Tanaka, Masato

AU - Takahashi, Hideo K.

AU - Nishibori, Masahiro

AU - Ozaki, Toshihumi

AU - Nishida, Keiichiro

PY - 2011/12/22

Y1 - 2011/12/22

N2 - We investigated the expression and localization of high-mobility group box chromosomal protein-1 (HMGB-1) in human osteoarthritic (OA) cartilage in relation to the histopathological grade of cartilage destruction, and examined the role of HMGB-1 in the regulation of proinflammatory cytokine expression in chondrocytes. An immunohistochemical study demonstrated that total HMGB-1-positive cell ratios increase as the Osteoarthritis Research Society International (OARSI) histological grade increased. The population of cytoplasmic HMGB-1-positive chondrocytes was especially increased in the deep layers of higher-grade cartilage. The ratios and localization of receptors for advanced glyca-tion end products (RAGE) expression by chondrocytes in Grade 2, 3, and 4 were significantly higher than those in Grade 1. In vitro stimulation with IL-1β, but not TNFα, significantly upregulated the expression of HMGB-1 mRNA by human OA chondrocytes. Both IL-1β and TNFα promoted the trans-location of HMGB-1 from nuclei to cytoplasm. IL-1β and TNFα secretions were stimulated at higher levels of HMGB-1. The results of our study suggest the involvement of HMGB-1 in the pathogenesis of cartilage destruction in OA.

AB - We investigated the expression and localization of high-mobility group box chromosomal protein-1 (HMGB-1) in human osteoarthritic (OA) cartilage in relation to the histopathological grade of cartilage destruction, and examined the role of HMGB-1 in the regulation of proinflammatory cytokine expression in chondrocytes. An immunohistochemical study demonstrated that total HMGB-1-positive cell ratios increase as the Osteoarthritis Research Society International (OARSI) histological grade increased. The population of cytoplasmic HMGB-1-positive chondrocytes was especially increased in the deep layers of higher-grade cartilage. The ratios and localization of receptors for advanced glyca-tion end products (RAGE) expression by chondrocytes in Grade 2, 3, and 4 were significantly higher than those in Grade 1. In vitro stimulation with IL-1β, but not TNFα, significantly upregulated the expression of HMGB-1 mRNA by human OA chondrocytes. Both IL-1β and TNFα promoted the trans-location of HMGB-1 from nuclei to cytoplasm. IL-1β and TNFα secretions were stimulated at higher levels of HMGB-1. The results of our study suggest the involvement of HMGB-1 in the pathogenesis of cartilage destruction in OA.

KW - Cartilage

KW - Chondrocyte

KW - HMGB-1

KW - Osteoarthritis

KW - RAGE

UR - http://www.scopus.com/inward/record.url?scp=84863266430&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863266430&partnerID=8YFLogxK

M3 - Article

VL - 65

SP - 369

EP - 378

JO - Acta Medica Okayama

JF - Acta Medica Okayama

SN - 0386-300X

IS - 6

ER -