@inbook{d954ba37896743338e031ba0690bfddb,
title = "Gene Expression Analysis of CCNs Along with Odontoblastic Differentiation In Vivo",
abstract = "Dental pulp cells (DPCs) differentiate into odontoblasts. To observe odontoblastic differentiation, the detection of dentinogenesis-specific molecules such as dentin sialophosphoprotein (DSPP) and the measurement of alkaline phosphatase (ALP) activity are reliable approaches. CCN family member 2 (CCN2) has been proposed as a marker for dentinogenesis. Our recent study revealed that the expression levels of Ccn4, Ccn5, and Ccn6 were changed in accordance with odontoblastic differentiation. Therefore, Ccn4, Ccn5, and Ccn6, as well as Ccn2, could serve as a comprehensive set of markers for dentinogenesis. Here, we describe a method of measuring the Ccns expression levels in differentiating rat DPCs.",
keywords = "Dental pulp cells, Dentinogenesis, Odontoblasts, Rat teeth, Real-time PCR, Western blot",
author = "Kazumi Kawata and Satoshi Kubota",
note = "Funding Information: This work was supported by the Grant-in-Aid for Scientific Research (C) 19K10109 (to K.K.). Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2744-0_19",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "281--291",
booktitle = "Methods in Molecular Biology",
}