Gene duplication and multiplicity of collagenases in Clostridium histolyticum

Osamu Matsushita, Chang Min Jung, Seiichi Katayama, Junzaburo Minami, Yukie Takahashi, Akinobu Okabe

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Clostridium histolyticum collagenase contains a number of different active components. Previously we have shown that colH encodes a 116-kDa collagenase (ColH) and a 98-kDa gelatinase. We purified a different 116-kDa collagenase (ColG) from the culture supernatant and sequenced its gene (colG). We also identified four other gelatinases (105, 82, 78, and 67 kDa) and determined their N-terminal amino acid sequences, all of which coincided with that of either ColG or ColH. Hybridization experiments showed that each gene is present in a single copy and each gene is transcribed into a single mRNA. These results suggest that all the gelatinases are produced from the respective full-length collagenase by the proteolytic removal of C-terminal fragments. The substrate specificities of the enzymes suggest that colG and colH encode class I and class II enzymes, respectively. Analysis of their DNA locations by pulsed-field gel electrophoresis and nucleotide sequencing of their surrounding regions revealed that the two genes are located in different sites on the chromosome. C. histolyticum colG is more similar to C. perfringens cola than to colH in terms of domain structure. Both colG and colA have a homologous gene, mscL, at their 3' ends. These results suggest that gene duplication and segment duplication have occurred in an ancestor cell common to C. histolyticum and C. perfringens and that further divergence of the parent gene produced colG and colA.

Original languageEnglish
Pages (from-to)923-933
Number of pages11
JournalJournal of Bacteriology
Volume181
Issue number3
Publication statusPublished - Feb 1999
Externally publishedYes

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Microbial Collagenase
Gene Duplication
Collagenases
Gelatinases
Clostridium histolyticum
Genes
Colicins
Pulsed Field Gel Electrophoresis
Enzymes
Substrate Specificity
Amino Acid Sequence
Nucleotides
Chromosomes
Messenger RNA
DNA

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Matsushita, O., Jung, C. M., Katayama, S., Minami, J., Takahashi, Y., & Okabe, A. (1999). Gene duplication and multiplicity of collagenases in Clostridium histolyticum. Journal of Bacteriology, 181(3), 923-933.

Gene duplication and multiplicity of collagenases in Clostridium histolyticum. / Matsushita, Osamu; Jung, Chang Min; Katayama, Seiichi; Minami, Junzaburo; Takahashi, Yukie; Okabe, Akinobu.

In: Journal of Bacteriology, Vol. 181, No. 3, 02.1999, p. 923-933.

Research output: Contribution to journalArticle

Matsushita, O, Jung, CM, Katayama, S, Minami, J, Takahashi, Y & Okabe, A 1999, 'Gene duplication and multiplicity of collagenases in Clostridium histolyticum', Journal of Bacteriology, vol. 181, no. 3, pp. 923-933.
Matsushita O, Jung CM, Katayama S, Minami J, Takahashi Y, Okabe A. Gene duplication and multiplicity of collagenases in Clostridium histolyticum. Journal of Bacteriology. 1999 Feb;181(3):923-933.
Matsushita, Osamu ; Jung, Chang Min ; Katayama, Seiichi ; Minami, Junzaburo ; Takahashi, Yukie ; Okabe, Akinobu. / Gene duplication and multiplicity of collagenases in Clostridium histolyticum. In: Journal of Bacteriology. 1999 ; Vol. 181, No. 3. pp. 923-933.
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