Gene delivery using nano-HAP/PVA/DNA complexes promoting endosomal escape

Yoichi Nibe, Tsuyoshi Kimura, Kwangwoo Nam, Shingo Mutsuo, Hidekazu Yoshizawa, Masahiro Okada, Tsutomu Furuzono, Toshiya Fujisato, Akio Kishida

Research output: Contribution to conferencePaper

Abstract

We have researched gene transfection using hydrogen bonding polymer/DNA complex prepared by ultra high pressure technology. In this study, for effective gene transfection, we investigated the preparation of nano-hydroxyl apatite (HAp)/PVA/DNA complex including nano-scaled HAp particles promoting endosomal escape. Gene transfection was carried out using fluorescent labeled plasmid DNA molecules. After 1hour incubation, the effective cellular uptake of HAp/PVA/DNA complexes comparison with PVA/DNA complex and DNA was observed by fluorescent microscope, and then high transfection efficiency was achieved using HAp/PVA/DNA complexes. These results indicate the effective DNA release from endocytosis.

Original languageEnglish
Number of pages1
Publication statusPublished - Oct 19 2006
Externally publishedYes
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: May 24 2006May 26 2006

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period5/24/065/26/06

Keywords

  • Hydrogen bond
  • Hydroxyl apatite
  • Inorganic nano-particles
  • Nano-composite / gene delivery
  • Ultra high pressure

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Nibe, Y., Kimura, T., Nam, K., Mutsuo, S., Yoshizawa, H., Okada, M., Furuzono, T., Fujisato, T., & Kishida, A. (2006). Gene delivery using nano-HAP/PVA/DNA complexes promoting endosomal escape. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.