Gene delivery using nano-HAP/PVA/DNA complexes promoting endosomal escape

Yoichi Nibe, Tsuyoshi Kimura, Kwangwoo Nam, Shingo Mutsuo, Hidekazu Yoshizawa, Masahiro Okada, Tsutomu Furuzono, Toshiya Fujisato, Akio Kishida

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have researched gene transfection using hydrogen bonding polymer/DNA complex prepared by ultra high pressure technology. In this study, for effective gene transfection, we investigated the preparation of nano-hydroxyl apatite (HAp)/PVA/DNA complex including nano-scaled HAp particles promoting endosomal escape. Gene transfection was carried out using fluorescent labeled plasmid DNA molecules. After 1hour incubation, the effective cellular uptake of HAp/PVA/DNA complexes comparison with PVA/DNA complex and DNA was observed by fluorescent microscope, and then high transfection efficiency was achieved using HAp/PVA/DNA complexes. These results indicate the effective DNA release from endocytosis.

Original languageEnglish
Title of host publicationPolymer Preprints, Japan
Pages2056
Number of pages1
Volume55
Edition1
Publication statusPublished - 2006
Externally publishedYes
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: May 24 2006May 26 2006

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period5/24/065/26/06

Fingerprint

DNA
Genes
Apatite
Hydrogen bonds
Microscopes
Molecules
Polymers

Keywords

  • Hydrogen bond
  • Hydroxyl apatite
  • Inorganic nano-particles
  • Nano-composite / gene delivery
  • Ultra high pressure

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Nibe, Y., Kimura, T., Nam, K., Mutsuo, S., Yoshizawa, H., Okada, M., ... Kishida, A. (2006). Gene delivery using nano-HAP/PVA/DNA complexes promoting endosomal escape. In Polymer Preprints, Japan (1 ed., Vol. 55, pp. 2056)

Gene delivery using nano-HAP/PVA/DNA complexes promoting endosomal escape. / Nibe, Yoichi; Kimura, Tsuyoshi; Nam, Kwangwoo; Mutsuo, Shingo; Yoshizawa, Hidekazu; Okada, Masahiro; Furuzono, Tsutomu; Fujisato, Toshiya; Kishida, Akio.

Polymer Preprints, Japan. Vol. 55 1. ed. 2006. p. 2056.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nibe, Y, Kimura, T, Nam, K, Mutsuo, S, Yoshizawa, H, Okada, M, Furuzono, T, Fujisato, T & Kishida, A 2006, Gene delivery using nano-HAP/PVA/DNA complexes promoting endosomal escape. in Polymer Preprints, Japan. 1 edn, vol. 55, pp. 2056, 55th SPSJ Annual Meeting, Nagoya, Japan, 5/24/06.
Nibe Y, Kimura T, Nam K, Mutsuo S, Yoshizawa H, Okada M et al. Gene delivery using nano-HAP/PVA/DNA complexes promoting endosomal escape. In Polymer Preprints, Japan. 1 ed. Vol. 55. 2006. p. 2056
Nibe, Yoichi ; Kimura, Tsuyoshi ; Nam, Kwangwoo ; Mutsuo, Shingo ; Yoshizawa, Hidekazu ; Okada, Masahiro ; Furuzono, Tsutomu ; Fujisato, Toshiya ; Kishida, Akio. / Gene delivery using nano-HAP/PVA/DNA complexes promoting endosomal escape. Polymer Preprints, Japan. Vol. 55 1. ed. 2006. pp. 2056
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