Gene cloning and characterization of KdeA, a multidrug efflux pump from Klebsiella pneumoniae

Yu Ping, Wakano Ogawa, Teruo Kuroda, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We cloned a gene related to multidrug resistance from a drug-resistant Klebsiella pneumoniae strain MGH78578. We designated the gene kdeA, which encodes a protein possessing 12 hydrophobic regions. The deduced amino acid sequence of KdeA is similar to that of MdfA, a well-characterized multidrug efflux pump from Escherichia coli. Introduction of the kdeA gene into cells of the drug-hypersusceptible E. coli strain KAM32 resulted in elevated minimum inhibitory concentrations of chloramphenicol, norfloxacin, acriflavine, and ethidium bromide. We observed elevated energy-dependent ethidium efflux activity with cells carrying kdeA compared with control cells. We also observed expression of kdeA in cells of K. pneumoniae under normal growth conditions.

Original languageEnglish
Pages (from-to)1962-1964
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume30
Issue number10
DOIs
Publication statusPublished - Oct 2007
Externally publishedYes

Keywords

  • KdeA
  • Klebsiella pneumoniae
  • Multidrug efflux pump
  • Multidrug resistance

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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