Abstract
We cloned a gene related to multidrug resistance from a drug-resistant Klebsiella pneumoniae strain MGH78578. We designated the gene kdeA, which encodes a protein possessing 12 hydrophobic regions. The deduced amino acid sequence of KdeA is similar to that of MdfA, a well-characterized multidrug efflux pump from Escherichia coli. Introduction of the kdeA gene into cells of the drug-hypersusceptible E. coli strain KAM32 resulted in elevated minimum inhibitory concentrations of chloramphenicol, norfloxacin, acriflavine, and ethidium bromide. We observed elevated energy-dependent ethidium efflux activity with cells carrying kdeA compared with control cells. We also observed expression of kdeA in cells of K. pneumoniae under normal growth conditions.
Original language | English |
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Pages (from-to) | 1962-1964 |
Number of pages | 3 |
Journal | Biological and Pharmaceutical Bulletin |
Volume | 30 |
Issue number | 10 |
DOIs | |
Publication status | Published - Oct 2007 |
Keywords
- KdeA
- Klebsiella pneumoniae
- Multidrug efflux pump
- Multidrug resistance
ASJC Scopus subject areas
- Pharmacology
- Pharmaceutical Science