Gene cloning and characterization of KdeA, a multidrug efflux pump from Klebsiella pneumoniae

Yu Ping; Wakano Ogawa; Teruo Kuroda; Tomofusa Tsuchiya

doi:10.1248/bpb.30.1962

Gene cloning and characterization of KdeA, a multidrug efflux pump from Klebsiella pneumoniae

Yu Ping, Wakano Ogawa, Teruo Kuroda, Tomofusa Tsuchiya

Faculty of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

We cloned a gene related to multidrug resistance from a drug-resistant Klebsiella pneumoniae strain MGH78578. We designated the gene kdeA, which encodes a protein possessing 12 hydrophobic regions. The deduced amino acid sequence of KdeA is similar to that of MdfA, a well-characterized multidrug efflux pump from Escherichia coli. Introduction of the kdeA gene into cells of the drug-hypersusceptible E. coli strain KAM32 resulted in elevated minimum inhibitory concentrations of chloramphenicol, norfloxacin, acriflavine, and ethidium bromide. We observed elevated energy-dependent ethidium efflux activity with cells carrying kdeA compared with control cells. We also observed expression of kdeA in cells of K. pneumoniae under normal growth conditions.

Original language	English
Pages (from-to)	1962-1964
Number of pages	3
Journal	Biological and Pharmaceutical Bulletin
Volume	30
Issue number	10
DOIs	https://doi.org/10.1248/bpb.30.1962
Publication status	Published - Oct 2007

Keywords

KdeA
Klebsiella pneumoniae
Multidrug efflux pump
Multidrug resistance

ASJC Scopus subject areas

Pharmacology
Pharmaceutical Science

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abstract = "We cloned a gene related to multidrug resistance from a drug-resistant Klebsiella pneumoniae strain MGH78578. We designated the gene kdeA, which encodes a protein possessing 12 hydrophobic regions. The deduced amino acid sequence of KdeA is similar to that of MdfA, a well-characterized multidrug efflux pump from Escherichia coli. Introduction of the kdeA gene into cells of the drug-hypersusceptible E. coli strain KAM32 resulted in elevated minimum inhibitory concentrations of chloramphenicol, norfloxacin, acriflavine, and ethidium bromide. We observed elevated energy-dependent ethidium efflux activity with cells carrying kdeA compared with control cells. We also observed expression of kdeA in cells of K. pneumoniae under normal growth conditions.",

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