Gelatin-siloxane hybrid scaffolds with vascular endothelial growth factor induces brain tissue regeneration

Hanzhe Zhang; Tatsushi Kamiya; Takeshi Hayashi; Kanji Tsuru; Kentaro Deguchi; Violeta Lukic; Atsushi Tsuchiya; Toru Yamashita; Satoshi Hayakawa; Yoshio Ikeda; Akiyoshi Osaka; Koji Abe

doi:10.2174/156720208784310204

Gelatin-siloxane hybrid scaffolds with vascular endothelial growth factor induces brain tissue regeneration

Hanzhe Zhang, Tatsushi Kamiya, Takeshi Hayashi, Kanji Tsuru, Kentaro Deguchi, Violeta Lukic, Atsushi Tsuchiya, Toru Yamashita, Satoshi Hayakawa, Yoshio Ikeda, Akiyoshi Osaka, Koji Abe

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

In the brain after infarction or trauma, the tissue becomes pannecrotic and forms a cavity. In such situation, a scaffold is necessary to produce new tissue. In this study, we implanted a new porous gelatin-siloxane hybrid derived from gelatin and 3-(glycidoxypropyl) trimethoxysilane (gelatin-GPTMS) scaffolds into a brain defect, and investigated whether it makes a new brain tissue. In addition, vascular endothelial growth factor (VEGF) was added on gelatin-GPTMS scaffolds and its effect on tissue regeneration was examined. At 30 days after the implantation, the marginal territory of the scaffolds became occupied by newly formed tissue. Immunohistochemical analysis revealed that the new tissue was constituted by endothelial, astroglial and microglial cells, some of which were labeled for bromodeoxyuridine (BrdU). Addition of VEGF promoted numbers of these cells. Thus, combination of gelatin-GPTMS scaffolds and VEGF is preferable for brain regeneration.

Original language	English
Pages (from-to)	112-117
Number of pages	6
Journal	Current Neurovascular Research
Volume	5
Issue number	2
DOIs	https://doi.org/10.2174/156720208784310204
Publication status	Published - May 2008

Keywords

Brain
Scaffold
Tissue regeneration
Vascular endothelial growth factor

ASJC Scopus subject areas

Neurology
Developmental Neuroscience
Cellular and Molecular Neuroscience

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Gelatin-siloxane hybrid scaffolds with vascular endothelial growth factor induces brain tissue regeneration. / Zhang, Hanzhe; Kamiya, Tatsushi; Hayashi, Takeshi; Tsuru, Kanji; Deguchi, Kentaro; Lukic, Violeta; Tsuchiya, Atsushi; Yamashita, Toru ; Hayakawa, Satoshi; Ikeda, Yoshio; Osaka, Akiyoshi; Abe, Koji.

In: Current Neurovascular Research, Vol. 5, No. 2, 05.2008, p. 112-117.

Research output: Contribution to journal › Article › peer-review

Zhang, Hanzhe ; Kamiya, Tatsushi ; Hayashi, Takeshi ; Tsuru, Kanji ; Deguchi, Kentaro ; Lukic, Violeta ; Tsuchiya, Atsushi ; Yamashita, Toru ; Hayakawa, Satoshi ; Ikeda, Yoshio ; Osaka, Akiyoshi ; Abe, Koji. / Gelatin-siloxane hybrid scaffolds with vascular endothelial growth factor induces brain tissue regeneration. In: Current Neurovascular Research. 2008 ; Vol. 5, No. 2. pp. 112-117.

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