Gelatin-siloxane hybrid scaffolds with vascular endothelial growth factor induces brain tissue regeneration

Hanzhe Zhang, Tatsushi Kamiya, Takeshi Hayashi, Kanji Tsuru, Kentaro Deguchi, Violeta Lukic, Atsushi Tsuchiya, Toru Yamashita, Satoshi Hayakawa, Yoshio Ikeda, Akiyoshi Osaka, Koji Abe

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In the brain after infarction or trauma, the tissue becomes pannecrotic and forms a cavity. In such situation, a scaffold is necessary to produce new tissue. In this study, we implanted a new porous gelatin-siloxane hybrid derived from gelatin and 3-(glycidoxypropyl) trimethoxysilane (gelatin-GPTMS) scaffolds into a brain defect, and investigated whether it makes a new brain tissue. In addition, vascular endothelial growth factor (VEGF) was added on gelatin-GPTMS scaffolds and its effect on tissue regeneration was examined. At 30 days after the implantation, the marginal territory of the scaffolds became occupied by newly formed tissue. Immunohistochemical analysis revealed that the new tissue was constituted by endothelial, astroglial and microglial cells, some of which were labeled for bromodeoxyuridine (BrdU). Addition of VEGF promoted numbers of these cells. Thus, combination of gelatin-GPTMS scaffolds and VEGF is preferable for brain regeneration.

Original languageEnglish
Pages (from-to)112-117
Number of pages6
JournalCurrent Neurovascular Research
Volume5
Issue number2
DOIs
Publication statusPublished - May 2008

Fingerprint

Siloxanes
Gelatin
Vascular Endothelial Growth Factor A
Regeneration
Brain
Brain Infarction
Bromodeoxyuridine
Cell Count
trimethoxysilane

Keywords

  • Brain
  • Scaffold
  • Tissue regeneration
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

Cite this

Gelatin-siloxane hybrid scaffolds with vascular endothelial growth factor induces brain tissue regeneration. / Zhang, Hanzhe; Kamiya, Tatsushi; Hayashi, Takeshi; Tsuru, Kanji; Deguchi, Kentaro; Lukic, Violeta; Tsuchiya, Atsushi; Yamashita, Toru; Hayakawa, Satoshi; Ikeda, Yoshio; Osaka, Akiyoshi; Abe, Koji.

In: Current Neurovascular Research, Vol. 5, No. 2, 05.2008, p. 112-117.

Research output: Contribution to journalArticle

Zhang, Hanzhe ; Kamiya, Tatsushi ; Hayashi, Takeshi ; Tsuru, Kanji ; Deguchi, Kentaro ; Lukic, Violeta ; Tsuchiya, Atsushi ; Yamashita, Toru ; Hayakawa, Satoshi ; Ikeda, Yoshio ; Osaka, Akiyoshi ; Abe, Koji. / Gelatin-siloxane hybrid scaffolds with vascular endothelial growth factor induces brain tissue regeneration. In: Current Neurovascular Research. 2008 ; Vol. 5, No. 2. pp. 112-117.
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