Abstract
In the brain after infarction or trauma, the tissue becomes pannecrotic and forms a cavity. In such situation, a scaffold is necessary to produce new tissue. In this study, we implanted a new porous gelatin-siloxane hybrid derived from gelatin and 3-(glycidoxypropyl) trimethoxysilane (gelatin-GPTMS) scaffolds into a brain defect, and investigated whether it makes a new brain tissue. In addition, vascular endothelial growth factor (VEGF) was added on gelatin-GPTMS scaffolds and its effect on tissue regeneration was examined. At 30 days after the implantation, the marginal territory of the scaffolds became occupied by newly formed tissue. Immunohistochemical analysis revealed that the new tissue was constituted by endothelial, astroglial and microglial cells, some of which were labeled for bromodeoxyuridine (BrdU). Addition of VEGF promoted numbers of these cells. Thus, combination of gelatin-GPTMS scaffolds and VEGF is preferable for brain regeneration.
| Original language | English |
|---|---|
| Pages (from-to) | 112-117 |
| Number of pages | 6 |
| Journal | Current Neurovascular Research |
| Volume | 5 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - May 2008 |
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Keywords
- Brain
- Scaffold
- Tissue regeneration
- Vascular endothelial growth factor
ASJC Scopus subject areas
- Neurology
- Clinical Neurology
Cite this
Gelatin-siloxane hybrid scaffolds with vascular endothelial growth factor induces brain tissue regeneration. / Zhang, Hanzhe; Kamiya, Tatsushi; Hayashi, Takeshi; Tsuru, Kanji; Deguchi, Kentaro; Lukic, Violeta; Tsuchiya, Atsushi; Yamashita, Toru; Hayakawa, Satoshi; Ikeda, Yoshio; Osaka, Akiyoshi; Abe, Koji.
In: Current Neurovascular Research, Vol. 5, No. 2, 05.2008, p. 112-117.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gelatin-siloxane hybrid scaffolds with vascular endothelial growth factor induces brain tissue regeneration
AU - Zhang, Hanzhe
AU - Kamiya, Tatsushi
AU - Hayashi, Takeshi
AU - Tsuru, Kanji
AU - Deguchi, Kentaro
AU - Lukic, Violeta
AU - Tsuchiya, Atsushi
AU - Yamashita, Toru
AU - Hayakawa, Satoshi
AU - Ikeda, Yoshio
AU - Osaka, Akiyoshi
AU - Abe, Koji
PY - 2008/5
Y1 - 2008/5
N2 - In the brain after infarction or trauma, the tissue becomes pannecrotic and forms a cavity. In such situation, a scaffold is necessary to produce new tissue. In this study, we implanted a new porous gelatin-siloxane hybrid derived from gelatin and 3-(glycidoxypropyl) trimethoxysilane (gelatin-GPTMS) scaffolds into a brain defect, and investigated whether it makes a new brain tissue. In addition, vascular endothelial growth factor (VEGF) was added on gelatin-GPTMS scaffolds and its effect on tissue regeneration was examined. At 30 days after the implantation, the marginal territory of the scaffolds became occupied by newly formed tissue. Immunohistochemical analysis revealed that the new tissue was constituted by endothelial, astroglial and microglial cells, some of which were labeled for bromodeoxyuridine (BrdU). Addition of VEGF promoted numbers of these cells. Thus, combination of gelatin-GPTMS scaffolds and VEGF is preferable for brain regeneration.
AB - In the brain after infarction or trauma, the tissue becomes pannecrotic and forms a cavity. In such situation, a scaffold is necessary to produce new tissue. In this study, we implanted a new porous gelatin-siloxane hybrid derived from gelatin and 3-(glycidoxypropyl) trimethoxysilane (gelatin-GPTMS) scaffolds into a brain defect, and investigated whether it makes a new brain tissue. In addition, vascular endothelial growth factor (VEGF) was added on gelatin-GPTMS scaffolds and its effect on tissue regeneration was examined. At 30 days after the implantation, the marginal territory of the scaffolds became occupied by newly formed tissue. Immunohistochemical analysis revealed that the new tissue was constituted by endothelial, astroglial and microglial cells, some of which were labeled for bromodeoxyuridine (BrdU). Addition of VEGF promoted numbers of these cells. Thus, combination of gelatin-GPTMS scaffolds and VEGF is preferable for brain regeneration.
KW - Brain
KW - Scaffold
KW - Tissue regeneration
KW - Vascular endothelial growth factor
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UR - http://www.scopus.com/inward/citedby.url?scp=45249121812&partnerID=8YFLogxK
U2 - 10.2174/156720208784310204
DO - 10.2174/156720208784310204
M3 - Article
VL - 5
SP - 112
EP - 117
JO - Current Neurovascular Research
JF - Current Neurovascular Research
SN - 1567-2026
IS - 2
ER -