Gefitinib decreases the synthesis of matrix metalloproteinase and the adhesion to extracellular matrix proteins of colon cancer cells

Daisaku Toda, Tetsuya Ota, Kazunori Tsukuda, Keitaro Watanabe, Toshiyuki Fujiyama, Masakazu Murakami, Minoru Naito, Nobuyoshi Shimizu

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Background: Adhesion to extracellular matrix (ECM) proteins and degradation of basement membranes by matrix metalloproteinase (MMP) play important roles in cancer metastasis. In this study, the effects of gefitinib on the enzymatic activity of MMP and adhesion to ECM proteins in the HT29 colon cancer cell line were investigated. Materials and Methods: Microtiter plates, coated with ECM proteins, were used to investigate the adhesion of cancer cells to ECM proteins. The expression of MMPs was examined by zymography and semi-quantitative RT-PCR. Results: Gefitinib inhibited MMP-9 and MMP-2 secretion and mRNA expression in HT29 cells. Gefitinib also reduced the ability to adhere to laminin and type IV collagen. These effects were observed at such low doses that gefitinib had neither an antiproliferative effect nor the ability to induce apoptosis. Conclusion: Gefitinib decreased the production of MMPs and the adhesion to ECM proteins, important steps associated with cancer metastasis. These results suggest that gefitinib may have antimetastatic activity in colon cancer.

Original languageEnglish
Pages (from-to)129-134
Number of pages6
JournalAnticancer research
Volume26
Issue number1 A
Publication statusPublished - Jan 2006
Externally publishedYes

Keywords

  • Adhesion
  • Colon cancer cell line
  • ECM
  • Gefitinib
  • MMP
  • RT-PCR

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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