GDNF-pretreatment enhances the survival of neural stem cells following transplantation in a rat model of Parkinson's disease

F. Wang, Masahiro Kameda, Takao Yasuhara, N. Tajiri, Y. Kikuchi, H. B. Liang, J. T. Tayra, A. Shinko, T. Wakamori, T. Agari, Isao Date

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Cell transplantation has been shown to be an effective therapy for central nervous system disorders in animal models. Improving the efficacy of cell transplantation depends critically on improving grafted cell survival. We investigated whether glial cell line-derived neurotrophic factor (GDNF)-pretreatment of neural stem cells (NSCs) enhanced grafted cell survival in a rat model of Parkinson's disease (PD). We first examined the neuroprotective effects of GDNF on oxygen-glucose deprivation (OGD) in NSCs. Cells were pretreated with GDNF for 3 days before subjecting them to OGD. After 12. h of OGD, GDNF-pretreated NSCs showed significant increases in survival rates compared with PBS-pretreated NSCs. An apoptosis assay showed that the number of apoptotic cells was significantly decreased in GDNF-pretreated NSCs at 1. h and 6. h after OGD. A PD rat model was then established by unilateral injection of 6-hydroxydopamine (6-OHDA, 9μg) into the medial forebrain bundle. Two weeks after 6-OHDA injection, GDNF-pretreated NSCs, PBS-pretreated NSCs, or PBS were injected into PD rat striatum. The survival of grafted cells in the striatum was significantly increased in the GDNF-pretreated NSC group compared with the control groups. GDNF pretreatment increased survival of NSCs following transplantation, at least partly through suppression of cell apoptosis.

Original languageEnglish
Pages (from-to)92-98
Number of pages7
JournalNeuroscience Research
Volume71
Issue number1
DOIs
Publication statusPublished - Sep 2011

Fingerprint

Glial Cell Line-Derived Neurotrophic Factor
Neural Stem Cells
Stem Cell Transplantation
Parkinson Disease
Oxidopamine
Oxygen
Glucose
Cell Survival
Cell Transplantation
Apoptosis
Medial Forebrain Bundle
Injections
Central Nervous System Diseases
Neuroprotective Agents
Animal Models
Cell Count
Control Groups

Keywords

  • Animal model
  • Cell survival
  • Cell transplantation
  • GDNF
  • Neural stem cell
  • Parkinson's disease

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

GDNF-pretreatment enhances the survival of neural stem cells following transplantation in a rat model of Parkinson's disease. / Wang, F.; Kameda, Masahiro; Yasuhara, Takao; Tajiri, N.; Kikuchi, Y.; Liang, H. B.; Tayra, J. T.; Shinko, A.; Wakamori, T.; Agari, T.; Date, Isao.

In: Neuroscience Research, Vol. 71, No. 1, 09.2011, p. 92-98.

Research output: Contribution to journalArticle

Wang, F. ; Kameda, Masahiro ; Yasuhara, Takao ; Tajiri, N. ; Kikuchi, Y. ; Liang, H. B. ; Tayra, J. T. ; Shinko, A. ; Wakamori, T. ; Agari, T. ; Date, Isao. / GDNF-pretreatment enhances the survival of neural stem cells following transplantation in a rat model of Parkinson's disease. In: Neuroscience Research. 2011 ; Vol. 71, No. 1. pp. 92-98.
@article{e05c9795e5cc43978ad01355e994c14a,
title = "GDNF-pretreatment enhances the survival of neural stem cells following transplantation in a rat model of Parkinson's disease",
abstract = "Cell transplantation has been shown to be an effective therapy for central nervous system disorders in animal models. Improving the efficacy of cell transplantation depends critically on improving grafted cell survival. We investigated whether glial cell line-derived neurotrophic factor (GDNF)-pretreatment of neural stem cells (NSCs) enhanced grafted cell survival in a rat model of Parkinson's disease (PD). We first examined the neuroprotective effects of GDNF on oxygen-glucose deprivation (OGD) in NSCs. Cells were pretreated with GDNF for 3 days before subjecting them to OGD. After 12. h of OGD, GDNF-pretreated NSCs showed significant increases in survival rates compared with PBS-pretreated NSCs. An apoptosis assay showed that the number of apoptotic cells was significantly decreased in GDNF-pretreated NSCs at 1. h and 6. h after OGD. A PD rat model was then established by unilateral injection of 6-hydroxydopamine (6-OHDA, 9μg) into the medial forebrain bundle. Two weeks after 6-OHDA injection, GDNF-pretreated NSCs, PBS-pretreated NSCs, or PBS were injected into PD rat striatum. The survival of grafted cells in the striatum was significantly increased in the GDNF-pretreated NSC group compared with the control groups. GDNF pretreatment increased survival of NSCs following transplantation, at least partly through suppression of cell apoptosis.",
keywords = "Animal model, Cell survival, Cell transplantation, GDNF, Neural stem cell, Parkinson's disease",
author = "F. Wang and Masahiro Kameda and Takao Yasuhara and N. Tajiri and Y. Kikuchi and Liang, {H. B.} and Tayra, {J. T.} and A. Shinko and T. Wakamori and T. Agari and Isao Date",
year = "2011",
month = "9",
doi = "10.1016/j.neures.2011.05.019",
language = "English",
volume = "71",
pages = "92--98",
journal = "Neuroscience Research",
issn = "0168-0102",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - GDNF-pretreatment enhances the survival of neural stem cells following transplantation in a rat model of Parkinson's disease

AU - Wang, F.

AU - Kameda, Masahiro

AU - Yasuhara, Takao

AU - Tajiri, N.

AU - Kikuchi, Y.

AU - Liang, H. B.

AU - Tayra, J. T.

AU - Shinko, A.

AU - Wakamori, T.

AU - Agari, T.

AU - Date, Isao

PY - 2011/9

Y1 - 2011/9

N2 - Cell transplantation has been shown to be an effective therapy for central nervous system disorders in animal models. Improving the efficacy of cell transplantation depends critically on improving grafted cell survival. We investigated whether glial cell line-derived neurotrophic factor (GDNF)-pretreatment of neural stem cells (NSCs) enhanced grafted cell survival in a rat model of Parkinson's disease (PD). We first examined the neuroprotective effects of GDNF on oxygen-glucose deprivation (OGD) in NSCs. Cells were pretreated with GDNF for 3 days before subjecting them to OGD. After 12. h of OGD, GDNF-pretreated NSCs showed significant increases in survival rates compared with PBS-pretreated NSCs. An apoptosis assay showed that the number of apoptotic cells was significantly decreased in GDNF-pretreated NSCs at 1. h and 6. h after OGD. A PD rat model was then established by unilateral injection of 6-hydroxydopamine (6-OHDA, 9μg) into the medial forebrain bundle. Two weeks after 6-OHDA injection, GDNF-pretreated NSCs, PBS-pretreated NSCs, or PBS were injected into PD rat striatum. The survival of grafted cells in the striatum was significantly increased in the GDNF-pretreated NSC group compared with the control groups. GDNF pretreatment increased survival of NSCs following transplantation, at least partly through suppression of cell apoptosis.

AB - Cell transplantation has been shown to be an effective therapy for central nervous system disorders in animal models. Improving the efficacy of cell transplantation depends critically on improving grafted cell survival. We investigated whether glial cell line-derived neurotrophic factor (GDNF)-pretreatment of neural stem cells (NSCs) enhanced grafted cell survival in a rat model of Parkinson's disease (PD). We first examined the neuroprotective effects of GDNF on oxygen-glucose deprivation (OGD) in NSCs. Cells were pretreated with GDNF for 3 days before subjecting them to OGD. After 12. h of OGD, GDNF-pretreated NSCs showed significant increases in survival rates compared with PBS-pretreated NSCs. An apoptosis assay showed that the number of apoptotic cells was significantly decreased in GDNF-pretreated NSCs at 1. h and 6. h after OGD. A PD rat model was then established by unilateral injection of 6-hydroxydopamine (6-OHDA, 9μg) into the medial forebrain bundle. Two weeks after 6-OHDA injection, GDNF-pretreated NSCs, PBS-pretreated NSCs, or PBS were injected into PD rat striatum. The survival of grafted cells in the striatum was significantly increased in the GDNF-pretreated NSC group compared with the control groups. GDNF pretreatment increased survival of NSCs following transplantation, at least partly through suppression of cell apoptosis.

KW - Animal model

KW - Cell survival

KW - Cell transplantation

KW - GDNF

KW - Neural stem cell

KW - Parkinson's disease

UR - http://www.scopus.com/inward/record.url?scp=79960929522&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960929522&partnerID=8YFLogxK

U2 - 10.1016/j.neures.2011.05.019

DO - 10.1016/j.neures.2011.05.019

M3 - Article

C2 - 21699926

AN - SCOPUS:79960929522

VL - 71

SP - 92

EP - 98

JO - Neuroscience Research

JF - Neuroscience Research

SN - 0168-0102

IS - 1

ER -