Abstract
Cell transplantation has been shown to be an effective therapy for central nervous system disorders in animal models. Improving the efficacy of cell transplantation depends critically on improving grafted cell survival. We investigated whether glial cell line-derived neurotrophic factor (GDNF)-pretreatment of neural stem cells (NSCs) enhanced grafted cell survival in a rat model of Parkinson's disease (PD). We first examined the neuroprotective effects of GDNF on oxygen-glucose deprivation (OGD) in NSCs. Cells were pretreated with GDNF for 3 days before subjecting them to OGD. After 12. h of OGD, GDNF-pretreated NSCs showed significant increases in survival rates compared with PBS-pretreated NSCs. An apoptosis assay showed that the number of apoptotic cells was significantly decreased in GDNF-pretreated NSCs at 1. h and 6. h after OGD. A PD rat model was then established by unilateral injection of 6-hydroxydopamine (6-OHDA, 9μg) into the medial forebrain bundle. Two weeks after 6-OHDA injection, GDNF-pretreated NSCs, PBS-pretreated NSCs, or PBS were injected into PD rat striatum. The survival of grafted cells in the striatum was significantly increased in the GDNF-pretreated NSC group compared with the control groups. GDNF pretreatment increased survival of NSCs following transplantation, at least partly through suppression of cell apoptosis.
| Original language | English |
|---|---|
| Pages (from-to) | 92-98 |
| Number of pages | 7 |
| Journal | Neuroscience Research |
| Volume | 71 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Sep 2011 |
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Keywords
- Animal model
- Cell survival
- Cell transplantation
- GDNF
- Neural stem cell
- Parkinson's disease
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
GDNF-pretreatment enhances the survival of neural stem cells following transplantation in a rat model of Parkinson's disease. / Wang, F.; Kameda, Masahiro; Yasuhara, Takao; Tajiri, N.; Kikuchi, Y.; Liang, H. B.; Tayra, J. T.; Shinko, A.; Wakamori, T.; Agari, T.; Date, Isao.
In: Neuroscience Research, Vol. 71, No. 1, 09.2011, p. 92-98.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - GDNF-pretreatment enhances the survival of neural stem cells following transplantation in a rat model of Parkinson's disease
AU - Wang, F.
AU - Kameda, Masahiro
AU - Yasuhara, Takao
AU - Tajiri, N.
AU - Kikuchi, Y.
AU - Liang, H. B.
AU - Tayra, J. T.
AU - Shinko, A.
AU - Wakamori, T.
AU - Agari, T.
AU - Date, Isao
PY - 2011/9
Y1 - 2011/9
N2 - Cell transplantation has been shown to be an effective therapy for central nervous system disorders in animal models. Improving the efficacy of cell transplantation depends critically on improving grafted cell survival. We investigated whether glial cell line-derived neurotrophic factor (GDNF)-pretreatment of neural stem cells (NSCs) enhanced grafted cell survival in a rat model of Parkinson's disease (PD). We first examined the neuroprotective effects of GDNF on oxygen-glucose deprivation (OGD) in NSCs. Cells were pretreated with GDNF for 3 days before subjecting them to OGD. After 12. h of OGD, GDNF-pretreated NSCs showed significant increases in survival rates compared with PBS-pretreated NSCs. An apoptosis assay showed that the number of apoptotic cells was significantly decreased in GDNF-pretreated NSCs at 1. h and 6. h after OGD. A PD rat model was then established by unilateral injection of 6-hydroxydopamine (6-OHDA, 9μg) into the medial forebrain bundle. Two weeks after 6-OHDA injection, GDNF-pretreated NSCs, PBS-pretreated NSCs, or PBS were injected into PD rat striatum. The survival of grafted cells in the striatum was significantly increased in the GDNF-pretreated NSC group compared with the control groups. GDNF pretreatment increased survival of NSCs following transplantation, at least partly through suppression of cell apoptosis.
AB - Cell transplantation has been shown to be an effective therapy for central nervous system disorders in animal models. Improving the efficacy of cell transplantation depends critically on improving grafted cell survival. We investigated whether glial cell line-derived neurotrophic factor (GDNF)-pretreatment of neural stem cells (NSCs) enhanced grafted cell survival in a rat model of Parkinson's disease (PD). We first examined the neuroprotective effects of GDNF on oxygen-glucose deprivation (OGD) in NSCs. Cells were pretreated with GDNF for 3 days before subjecting them to OGD. After 12. h of OGD, GDNF-pretreated NSCs showed significant increases in survival rates compared with PBS-pretreated NSCs. An apoptosis assay showed that the number of apoptotic cells was significantly decreased in GDNF-pretreated NSCs at 1. h and 6. h after OGD. A PD rat model was then established by unilateral injection of 6-hydroxydopamine (6-OHDA, 9μg) into the medial forebrain bundle. Two weeks after 6-OHDA injection, GDNF-pretreated NSCs, PBS-pretreated NSCs, or PBS were injected into PD rat striatum. The survival of grafted cells in the striatum was significantly increased in the GDNF-pretreated NSC group compared with the control groups. GDNF pretreatment increased survival of NSCs following transplantation, at least partly through suppression of cell apoptosis.
KW - Animal model
KW - Cell survival
KW - Cell transplantation
KW - GDNF
KW - Neural stem cell
KW - Parkinson's disease
UR - http://www.scopus.com/inward/record.url?scp=79960929522&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79960929522&partnerID=8YFLogxK
U2 - 10.1016/j.neures.2011.05.019
DO - 10.1016/j.neures.2011.05.019
M3 - Article
C2 - 21699926
AN - SCOPUS:79960929522
VL - 71
SP - 92
EP - 98
JO - Neuroscience Research
JF - Neuroscience Research
SN - 0168-0102
IS - 1
ER -