GC/MS-based profiling of amino acids and TCA cycle-related molecules in ulcerative colitis

Makoto Ooi, Shin Nishiumi, Tomoo Yoshie, Yuuki Shiomi, Michitaka Kohashi, Ken Fukunaga, Shiro Nakamura, Takayuki Matsumoto, Naoya Hatano, Masakazu Shinohara, Yasuhiro Irino, Tadaomi Takenawa, Takeshi Azuma, Masaru Yoshida

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Objective: The roles that amino acids play in immunity and inflammation are well defined, and the relationship between inflammatory bowel disease (IBD) and certain amino acids has recently attracted attention. In this study, the levels of amino acids and trichloroacetic acid (TCA) cycle-related molecules in the colonic tissues and sera of patients with ulcerative colitis (UC) were profiled by gas chromatography/mass spectrometry (GC/MS), with the aim of evaluating whether the clinical state induced by UC leads to variations in the amino acid profile. Materials and methods: Colonic biopsy samples from 22 UC patients were used, as well as serum samples from UC patients (n = 13), Crohn's disease (CD) patients (n = 21), and healthy volunteers (n = 17). Results: In the GC/MS-based profiling of amino acids and TCA cycle-related molecules, lower levels of 16 amino acids and 5 TCA cycle-related molecules were observed in the colonic lesion tissues of the UC patients, and the serum profiles of amino acids and TCA cycle-related molecules of the UC patients were different from those of the CD patients and healthy volunteers. Conclusions: Our study raises the possibility that GC/MS-based profiling of amino acids and TCA cycle-related molecules is a useful early diagnostic tool for UC.

Original languageEnglish
Pages (from-to)831-840
Number of pages10
JournalInflammation Research
Volume60
Issue number9
DOIs
Publication statusPublished - Sep 1 2011
Externally publishedYes

Keywords

  • Amino acid
  • GC/MS
  • Metabolomics
  • Ulcerative colitis

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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  • Cite this

    Ooi, M., Nishiumi, S., Yoshie, T., Shiomi, Y., Kohashi, M., Fukunaga, K., Nakamura, S., Matsumoto, T., Hatano, N., Shinohara, M., Irino, Y., Takenawa, T., Azuma, T., & Yoshida, M. (2011). GC/MS-based profiling of amino acids and TCA cycle-related molecules in ulcerative colitis. Inflammation Research, 60(9), 831-840. https://doi.org/10.1007/s00011-011-0340-7