GbEXPATR, a species-specific expansin, enhances cotton fibre elongation through cell wall restructuring

Yang Li, Lili Tu, Filomena A. Pettolino, Shengmei Ji, Juan Hao, Daojun Yuan, Fenglin Deng, Jiafu Tan, Haiyan Hu, Qing Wang, Danny J. Llewellyn, Xianlong Zhang

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Cotton provides us the most important natural fibre. High fibre quality is the major goal of cotton breeding, and introducing genes conferring longer, finer and stronger fibre from Gossypium barbadense to Gossypium hirsutum is an important breeding strategy. We previously analysed the G. barbadense fibre development mechanism by gene expression profiling and found two homoeologous fibre-specific α-expansins from G. barbadense, GbEXPA2 and GbEXPATR. GbEXPA2 (from the DT genome) is a classical α-expansin, while its homoeolog, GbEXPATR (AT genome), encodes a truncated protein lacking the normal C-terminal polysaccharide-binding domain of other α-expansins and is specifically expressed in G. barbadense. Silencing EXPA in G. hirsutum induced shorter fibres with thicker cell walls. GbEXPA2 overexpression in G. hirsutum had no effect on mature fibre length, but produced fibres with a slightly thicker wall and increased crystalline cellulose content. Interestingly, GbEXPATR overexpression resulted in longer, finer and stronger fibres coupled with significantly thinner cell walls. The longer and thinner fibre was associated with lower expression of a number of secondary wall-associated genes, especially chitinase-like genes, and walls with lower cellulose levels but higher noncellulosic polysaccharides which advocated that a delay in the transition to secondary wall synthesis might be responsible for better fibre. In conclusion, we propose that α-expansins play a critical role in fibre development by loosening the cell wall; furthermore, a truncated form, GbEXPATR, has a more dramatic effect through reorganizing secondary wall synthesis and metabolism and should be a candidate gene for developing G. hirsutum cultivars with superior fibre quality.

Original languageEnglish
Pages (from-to)951-963
Number of pages13
JournalPlant Biotechnology Journal
Volume14
Issue number3
DOIs
Publication statusPublished - Mar 1 2016
Externally publishedYes

Fingerprint

Cotton Fiber
Gossypium barbadense
expansins
lint cotton
Gossypium hirsutum
Cell Wall
cell walls
Gossypium
fiber quality
Cellulose
Genes
Breeding
Polysaccharides
cellulose
polysaccharides
genes
cotton
Genome
natural fibers
Chitinases

Keywords

  • Cell elongation
  • Cotton fibre
  • Gossypium barbadense (cotton)
  • Secondary cell wall
  • Truncated α-expansin

ASJC Scopus subject areas

  • Plant Science
  • Biotechnology
  • Agronomy and Crop Science

Cite this

GbEXPATR, a species-specific expansin, enhances cotton fibre elongation through cell wall restructuring. / Li, Yang; Tu, Lili; Pettolino, Filomena A.; Ji, Shengmei; Hao, Juan; Yuan, Daojun; Deng, Fenglin; Tan, Jiafu; Hu, Haiyan; Wang, Qing; Llewellyn, Danny J.; Zhang, Xianlong.

In: Plant Biotechnology Journal, Vol. 14, No. 3, 01.03.2016, p. 951-963.

Research output: Contribution to journalArticle

Li, Y, Tu, L, Pettolino, FA, Ji, S, Hao, J, Yuan, D, Deng, F, Tan, J, Hu, H, Wang, Q, Llewellyn, DJ & Zhang, X 2016, 'GbEXPATR, a species-specific expansin, enhances cotton fibre elongation through cell wall restructuring', Plant Biotechnology Journal, vol. 14, no. 3, pp. 951-963. https://doi.org/10.1111/pbi.12450
Li, Yang ; Tu, Lili ; Pettolino, Filomena A. ; Ji, Shengmei ; Hao, Juan ; Yuan, Daojun ; Deng, Fenglin ; Tan, Jiafu ; Hu, Haiyan ; Wang, Qing ; Llewellyn, Danny J. ; Zhang, Xianlong. / GbEXPATR, a species-specific expansin, enhances cotton fibre elongation through cell wall restructuring. In: Plant Biotechnology Journal. 2016 ; Vol. 14, No. 3. pp. 951-963.
@article{b9ade8d05793447da1e14566525d83f3,
title = "GbEXPATR, a species-specific expansin, enhances cotton fibre elongation through cell wall restructuring",
abstract = "Cotton provides us the most important natural fibre. High fibre quality is the major goal of cotton breeding, and introducing genes conferring longer, finer and stronger fibre from Gossypium barbadense to Gossypium hirsutum is an important breeding strategy. We previously analysed the G. barbadense fibre development mechanism by gene expression profiling and found two homoeologous fibre-specific α-expansins from G. barbadense, GbEXPA2 and GbEXPATR. GbEXPA2 (from the DT genome) is a classical α-expansin, while its homoeolog, GbEXPATR (AT genome), encodes a truncated protein lacking the normal C-terminal polysaccharide-binding domain of other α-expansins and is specifically expressed in G. barbadense. Silencing EXPA in G. hirsutum induced shorter fibres with thicker cell walls. GbEXPA2 overexpression in G. hirsutum had no effect on mature fibre length, but produced fibres with a slightly thicker wall and increased crystalline cellulose content. Interestingly, GbEXPATR overexpression resulted in longer, finer and stronger fibres coupled with significantly thinner cell walls. The longer and thinner fibre was associated with lower expression of a number of secondary wall-associated genes, especially chitinase-like genes, and walls with lower cellulose levels but higher noncellulosic polysaccharides which advocated that a delay in the transition to secondary wall synthesis might be responsible for better fibre. In conclusion, we propose that α-expansins play a critical role in fibre development by loosening the cell wall; furthermore, a truncated form, GbEXPATR, has a more dramatic effect through reorganizing secondary wall synthesis and metabolism and should be a candidate gene for developing G. hirsutum cultivars with superior fibre quality.",
keywords = "Cell elongation, Cotton fibre, Gossypium barbadense (cotton), Secondary cell wall, Truncated α-expansin",
author = "Yang Li and Lili Tu and Pettolino, {Filomena A.} and Shengmei Ji and Juan Hao and Daojun Yuan and Fenglin Deng and Jiafu Tan and Haiyan Hu and Qing Wang and Llewellyn, {Danny J.} and Xianlong Zhang",
year = "2016",
month = "3",
day = "1",
doi = "10.1111/pbi.12450",
language = "English",
volume = "14",
pages = "951--963",
journal = "Plant Biotechnology Journal",
issn = "1467-7644",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - GbEXPATR, a species-specific expansin, enhances cotton fibre elongation through cell wall restructuring

AU - Li, Yang

AU - Tu, Lili

AU - Pettolino, Filomena A.

AU - Ji, Shengmei

AU - Hao, Juan

AU - Yuan, Daojun

AU - Deng, Fenglin

AU - Tan, Jiafu

AU - Hu, Haiyan

AU - Wang, Qing

AU - Llewellyn, Danny J.

AU - Zhang, Xianlong

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Cotton provides us the most important natural fibre. High fibre quality is the major goal of cotton breeding, and introducing genes conferring longer, finer and stronger fibre from Gossypium barbadense to Gossypium hirsutum is an important breeding strategy. We previously analysed the G. barbadense fibre development mechanism by gene expression profiling and found two homoeologous fibre-specific α-expansins from G. barbadense, GbEXPA2 and GbEXPATR. GbEXPA2 (from the DT genome) is a classical α-expansin, while its homoeolog, GbEXPATR (AT genome), encodes a truncated protein lacking the normal C-terminal polysaccharide-binding domain of other α-expansins and is specifically expressed in G. barbadense. Silencing EXPA in G. hirsutum induced shorter fibres with thicker cell walls. GbEXPA2 overexpression in G. hirsutum had no effect on mature fibre length, but produced fibres with a slightly thicker wall and increased crystalline cellulose content. Interestingly, GbEXPATR overexpression resulted in longer, finer and stronger fibres coupled with significantly thinner cell walls. The longer and thinner fibre was associated with lower expression of a number of secondary wall-associated genes, especially chitinase-like genes, and walls with lower cellulose levels but higher noncellulosic polysaccharides which advocated that a delay in the transition to secondary wall synthesis might be responsible for better fibre. In conclusion, we propose that α-expansins play a critical role in fibre development by loosening the cell wall; furthermore, a truncated form, GbEXPATR, has a more dramatic effect through reorganizing secondary wall synthesis and metabolism and should be a candidate gene for developing G. hirsutum cultivars with superior fibre quality.

AB - Cotton provides us the most important natural fibre. High fibre quality is the major goal of cotton breeding, and introducing genes conferring longer, finer and stronger fibre from Gossypium barbadense to Gossypium hirsutum is an important breeding strategy. We previously analysed the G. barbadense fibre development mechanism by gene expression profiling and found two homoeologous fibre-specific α-expansins from G. barbadense, GbEXPA2 and GbEXPATR. GbEXPA2 (from the DT genome) is a classical α-expansin, while its homoeolog, GbEXPATR (AT genome), encodes a truncated protein lacking the normal C-terminal polysaccharide-binding domain of other α-expansins and is specifically expressed in G. barbadense. Silencing EXPA in G. hirsutum induced shorter fibres with thicker cell walls. GbEXPA2 overexpression in G. hirsutum had no effect on mature fibre length, but produced fibres with a slightly thicker wall and increased crystalline cellulose content. Interestingly, GbEXPATR overexpression resulted in longer, finer and stronger fibres coupled with significantly thinner cell walls. The longer and thinner fibre was associated with lower expression of a number of secondary wall-associated genes, especially chitinase-like genes, and walls with lower cellulose levels but higher noncellulosic polysaccharides which advocated that a delay in the transition to secondary wall synthesis might be responsible for better fibre. In conclusion, we propose that α-expansins play a critical role in fibre development by loosening the cell wall; furthermore, a truncated form, GbEXPATR, has a more dramatic effect through reorganizing secondary wall synthesis and metabolism and should be a candidate gene for developing G. hirsutum cultivars with superior fibre quality.

KW - Cell elongation

KW - Cotton fibre

KW - Gossypium barbadense (cotton)

KW - Secondary cell wall

KW - Truncated α-expansin

UR - http://www.scopus.com/inward/record.url?scp=84958892994&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84958892994&partnerID=8YFLogxK

U2 - 10.1111/pbi.12450

DO - 10.1111/pbi.12450

M3 - Article

AN - SCOPUS:84958892994

VL - 14

SP - 951

EP - 963

JO - Plant Biotechnology Journal

JF - Plant Biotechnology Journal

SN - 1467-7644

IS - 3

ER -