Gap-junction-mediated communication in human periodontal ligament cells

R. Kato, Yoshihito Ishihara, Noriaki Kawanabe, K. Sumiyoshi, Y. Yoshikawa, Masahiro Nakamura, Y. Imai, T. Yanagita, H. Fukushima, Hiroshi Kamioka, T. Takano-Yamamoto, T. Yamashiro

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Periodontal tissue homeostasis depends on a complex cellular network that conveys cell-cell communication. Gap junctions (GJs), one of the intercellular communication systems, are found between adjacent human periodontal ligament (hPDL) cells; however, the functional GJ coupling between hPDL cells has not yet been elucidated. In this study, we investigated functional gap-junction- mediated intercellular communication in isolated primary hPDL cells. SEM images indicated that the cells were in contact with each other via dendritic processes, and also showed high anti-connexin43 (Cx43) immunoreactivity on these processes. Gap-junctional intercellular communication (GJIC) among hPDL cells was assessed by fluorescence recovery after a photobleaching (FRAP) analysis, which exhibited dye coupling between hPDL cells, and was remarkably down-regulated when the cells were treated with a GJ blocker. Additionally, we examined GJs under hypoxic stress. The fluorescence recovery and expression levels of Cx43 decreased time-dependently under the hypoxic condition. Exposure to GJ inhibitor or hypoxia increased RANKL expression, and decreased OPG expression. This study shows that GJIC is responsible for hPDL cells and that its activity is reduced under hypoxia. This is consistent with the possible role of hPDL cells in regulating the biochemical reactions in response to changes in the hypoxic environment.

Original languageEnglish
Pages (from-to)635-640
Number of pages6
JournalJournal of Dental Research
Volume92
Issue number7
DOIs
Publication statusPublished - Jul 2013

Fingerprint

Periodontal Ligament
Gap Junctions
Communication
Connexin 43
Fluorescence Recovery After Photobleaching
Cell Communication
Homeostasis
Coloring Agents
Fluorescence

Keywords

  • cell communication
  • Connexin43
  • fluorescence recovery after photobleaching
  • gap junctions
  • hypoxia
  • periodontal ligament

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Gap-junction-mediated communication in human periodontal ligament cells. / Kato, R.; Ishihara, Yoshihito; Kawanabe, Noriaki; Sumiyoshi, K.; Yoshikawa, Y.; Nakamura, Masahiro; Imai, Y.; Yanagita, T.; Fukushima, H.; Kamioka, Hiroshi; Takano-Yamamoto, T.; Yamashiro, T.

In: Journal of Dental Research, Vol. 92, No. 7, 07.2013, p. 635-640.

Research output: Contribution to journalArticle

Kato, R, Ishihara, Y, Kawanabe, N, Sumiyoshi, K, Yoshikawa, Y, Nakamura, M, Imai, Y, Yanagita, T, Fukushima, H, Kamioka, H, Takano-Yamamoto, T & Yamashiro, T 2013, 'Gap-junction-mediated communication in human periodontal ligament cells', Journal of Dental Research, vol. 92, no. 7, pp. 635-640. https://doi.org/10.1177/0022034513489992
Kato, R. ; Ishihara, Yoshihito ; Kawanabe, Noriaki ; Sumiyoshi, K. ; Yoshikawa, Y. ; Nakamura, Masahiro ; Imai, Y. ; Yanagita, T. ; Fukushima, H. ; Kamioka, Hiroshi ; Takano-Yamamoto, T. ; Yamashiro, T. / Gap-junction-mediated communication in human periodontal ligament cells. In: Journal of Dental Research. 2013 ; Vol. 92, No. 7. pp. 635-640.
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