Gamma-interferon upregulates transferrin receptors and decreases membrane microviscosity in K562 cells

Hiromi Iwagaki, Masahito Marutaka, Tadashi Yoshino, Liu Cao, Isao Sakuma, Masashi Uomoto, Yoshiaki Takeuchi, Noriaki Tanaka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We investigated the effect of gamma interferon (γ-IFN) on three cellular parameters: cell membrane fluidity and expression of two antigens that have been associated with cell proliferation, namely transferrin receptor (Tf-R: a cell surface protein) and Ki-67 antigen (Ki-67: a nuclear protein). We observed small, yet significant changes in the first two parameters, but not the third parameter. These were investigated in K562 cells, a human chronic myelocytic leukemia cell line. These results suggest that the microviscosity changes and the surface Tf-R density were closely associated, and that γ- IFN was involved in increasing proliferative activity of the cells by decreasing membrane fluidity and upregulating Tf-R expression.

Original languageEnglish
Pages (from-to)191-200
Number of pages10
JournalResearch Communications in Molecular Pathology and Pharmacology
Volume92
Issue number2
Publication statusPublished - May 1996

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Membrane Fluidity
Transferrin Receptors
K562 Cells
Interferon-gamma
Up-Regulation
Cell Membrane
Ki-67 Antigen
Membranes
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Nuclear Proteins
Membrane Proteins
Cell Proliferation
Antigens
Cell Line

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pathology and Forensic Medicine

Cite this

Gamma-interferon upregulates transferrin receptors and decreases membrane microviscosity in K562 cells. / Iwagaki, Hiromi; Marutaka, Masahito; Yoshino, Tadashi; Cao, Liu; Sakuma, Isao; Uomoto, Masashi; Takeuchi, Yoshiaki; Tanaka, Noriaki.

In: Research Communications in Molecular Pathology and Pharmacology, Vol. 92, No. 2, 05.1996, p. 191-200.

Research output: Contribution to journalArticle

Iwagaki, H, Marutaka, M, Yoshino, T, Cao, L, Sakuma, I, Uomoto, M, Takeuchi, Y & Tanaka, N 1996, 'Gamma-interferon upregulates transferrin receptors and decreases membrane microviscosity in K562 cells', Research Communications in Molecular Pathology and Pharmacology, vol. 92, no. 2, pp. 191-200.
Iwagaki, Hiromi ; Marutaka, Masahito ; Yoshino, Tadashi ; Cao, Liu ; Sakuma, Isao ; Uomoto, Masashi ; Takeuchi, Yoshiaki ; Tanaka, Noriaki. / Gamma-interferon upregulates transferrin receptors and decreases membrane microviscosity in K562 cells. In: Research Communications in Molecular Pathology and Pharmacology. 1996 ; Vol. 92, No. 2. pp. 191-200.
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