GalNAc pretreatment inhibits trapping of Bacillus thuringiensis Cry1Ac on the peritrophic membrane of Bombyx mori

Tohru Hayakawa, Yasuyuki Shitomi, Kazuhisa Miyamoto, Hidetaka Hori

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Bombyx mori (Shunrei×Shogetsu) is sensitive to Cry1Aa and resistant to Cry1Ac, both insecticidal proteins of Bacillus thuringiensis. Cry1Aa passed through the peritrophic membrane (PM) much faster (0.37 μg/mm2 PM/h) than Cry1Ac (0.05 μg/mm2 PM/h) during the initial observation period. Both Cry1Aa and Cry1Ac bound to the PM but only the binding of Cry1Ac was specifically inhibited by N-acetylgalactosamine (GalNAc). When Cry1Ac was pretreated with GalNAc, Cry1Ac permeated the PM much faster. These results suggested that Cry1Ac bound a PM protein via GalNAc on a sugar side chain. The role of the PM on Cry1Ac resistance of B. mori was briefly discussed.

Original languageEnglish
Pages (from-to)331-335
Number of pages5
JournalFEBS Letters
Volume576
Issue number3
DOIs
Publication statusPublished - Oct 22 2004
Externally publishedYes

Keywords

  • Bacillus thuringiensis
  • Bombyx mori
  • N-acetylgalactosamine
  • Peritrophic membrane protein
  • Permeability of peritrophic membrane
  • δ-Endotoxin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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