Gallium-68-Labeled Anti-HER2 Single-Chain Fv Fragment: Development and In Vivo Monitoring of HER2 Expression

Masashi Ueda, Hayato Hisada, Takashi Temma, Yoichi Shimizu, Hiroyuki Kimura, Masahiro Ono, Yuji Nakamoto, Kaori Togashi, Hideo Saji

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Purpose: We aimed to develop a gallium-68 (Ga-68)-labeled single-chain variable fragment (scFv) targeting the human epidermal growth factor receptor 2 (HER2) to rapidly and noninvasively evaluate the status of HER2 expression.Procedures: Anti-HER2 scFv was labeled with Ga-68 by using deferoxamine (Df) as a bifunctional chelate. Biodistribution of [68Ga]Df-anti-HER2 scFv was examined with tumor-bearing mice and positron emission tomography (PET) imaging was performed. The changes in HER2 expression after anti-HER2 therapy were monitored by PET imaging.Results: [68Ga]Df-anti-HER2 scFv was obtained with high radiochemical yield after only a 5-min reaction at room temperature. The probe showed high accumulation in HER2-positive xenografts and the intratumoral distribution of radioactivity coincided with HER2-positive regions. Furthermore, [68Ga]Df-anti-HER2 scFv helped visualize HER2-positive xenografts and monitor the changes in HER2 expression after anti-HER2 therapy.Conclusion: [68Ga]Df-anti-HER2 scFv could be a promising probe to evaluate HER2 status by in vivo PET imaging, unless trastuzumab is prescribed as part of the therapy.

Original languageEnglish
Pages (from-to)102-110
Number of pages9
JournalMolecular Imaging and Biology
Volume17
Issue number1
DOIs
Publication statusPublished - 2014

Keywords

  • 17-DMAG
  • Gallium-68
  • Human epidermal growth factor receptor 2(HER2)
  • Positron emission tomography (PET)
  • Single-chain Fv Fragment (scFv)
  • Therapy

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

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    Ueda, M., Hisada, H., Temma, T., Shimizu, Y., Kimura, H., Ono, M., Nakamoto, Y., Togashi, K., & Saji, H. (2014). Gallium-68-Labeled Anti-HER2 Single-Chain Fv Fragment: Development and In Vivo Monitoring of HER2 Expression. Molecular Imaging and Biology, 17(1), 102-110. https://doi.org/10.1007/s11307-014-0769-5