Galectin-9 expands immunosuppressive macrophages to ameliorate T-cell-mediated lung inflammation

Tomohiro Arikawa, Naoki Saita, Souichi Oomizu, Masaki Ueno, Akihiro Matsukawa, Shigeki Katoh, Keisuke Kojima, Keiko Nagahara, Minoru Miyake, Akira Yamauchi, Hirotsugu Kohrogi, Mitsuomi Hirashima

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Galectin-9 (Gal-9) plays pivotal roles in the modulation of innate and adaptive immunity to suppress T-cell-mediated autoimmune models. However, it remains unclear if Gal-9 plays a suppressive role for T-cell function in non-autoimmune disease models. We assessed the effects of Gal-9 on experimental hypersensitivity pneumonitis induced by Trichosporon asahii. When Gal-9 was given subcutaneously to C57BL/6 mice at the time of challenge with T. asahii, it significantly suppressed T. asahii-induced lung inflammation, as the levels of IL-1, IL-6, IFN-γ, and IL-17 were significantly reduced in the BALF of Gal-9-treated mice. Moreover, co-culture of anti-CD3-stimulated CD4 T cells with BALF cells harvested from Gal-9-treated mice on day 1 resulted in diminished CD4 T-cell proliferation and decreased levels of IFN-γ and IL-17. CD11b+ Ly-6ChighF4/80+ BALF M/ expanded by Gal-9 were responsible for the suppression.We further found in vitro that Gal-9, only in the presence of T. asahii, expands CD11b+Ly-6C highF4/80+ cells from BM cells, and the cells suppress T-cell proliferation and IFN-γ and IL-17 production. The present results indicate that Gal-9 expands immunosuppressive CD11b+Ly-6C high Mφ to ameliorate Th1/Th17 cell-mediated hypersensitivity pneumonitis.

Original languageEnglish
Pages (from-to)548-558
Number of pages11
JournalEuropean Journal of Immunology
Volume40
Issue number2
DOIs
Publication statusPublished - Feb 2010

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Galectins
Immunosuppressive Agents
Pneumonia
Macrophages
T-Lymphocytes
Interleukin-17
Extrinsic Allergic Alveolitis
Cell Proliferation
Trichosporon
Th17 Cells
Th1 Cells
Adaptive Immunity
Coculture Techniques
Interleukin-1
Inbred C57BL Mouse
Innate Immunity
Interleukin-6

Keywords

  • Galectin-9
  • Immune suppression
  • Lung
  • Macrophage
  • Pneumonitis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Galectin-9 expands immunosuppressive macrophages to ameliorate T-cell-mediated lung inflammation. / Arikawa, Tomohiro; Saita, Naoki; Oomizu, Souichi; Ueno, Masaki; Matsukawa, Akihiro; Katoh, Shigeki; Kojima, Keisuke; Nagahara, Keiko; Miyake, Minoru; Yamauchi, Akira; Kohrogi, Hirotsugu; Hirashima, Mitsuomi.

In: European Journal of Immunology, Vol. 40, No. 2, 02.2010, p. 548-558.

Research output: Contribution to journalArticle

Arikawa, T, Saita, N, Oomizu, S, Ueno, M, Matsukawa, A, Katoh, S, Kojima, K, Nagahara, K, Miyake, M, Yamauchi, A, Kohrogi, H & Hirashima, M 2010, 'Galectin-9 expands immunosuppressive macrophages to ameliorate T-cell-mediated lung inflammation', European Journal of Immunology, vol. 40, no. 2, pp. 548-558. https://doi.org/10.1002/eji.200939886
Arikawa, Tomohiro ; Saita, Naoki ; Oomizu, Souichi ; Ueno, Masaki ; Matsukawa, Akihiro ; Katoh, Shigeki ; Kojima, Keisuke ; Nagahara, Keiko ; Miyake, Minoru ; Yamauchi, Akira ; Kohrogi, Hirotsugu ; Hirashima, Mitsuomi. / Galectin-9 expands immunosuppressive macrophages to ameliorate T-cell-mediated lung inflammation. In: European Journal of Immunology. 2010 ; Vol. 40, No. 2. pp. 548-558.
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