G-CSF reduces IFN-γ and IL-4 production by T cells after allogeneic stimulation by indirectly modulating monocyte function

Y. Nawa, T. Teshima, K. Sunami, Y. Hiramatsu, Yoshinobu Maeda, T. Yano, K. Shinagawa, F. Ishimaru, E. Omoto, M. Harada

Research output: Contribution to journalArticle

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Abstract

Despite a 10-fold increase of T cell dose, the incidence and severity of acute GVHD following allogeneic transplantation of G-CSF-mobilized PBSC is not increased compared to BMT. Experimental murine studies demonstrate that G-CSF polarizes donor T cells toward a type 2 cytokine response. To determine whether G-CSF alters T cell cytokine responses, we investigated the effects of G-CSF administration on T cell proliferative and cytokine responses to alloantigen and Con A in non-adherent PBMC (NAC) and CD3+ T cells obtained from normal individuals before and after G-CSF administration (10 μg/kg x 4 days). Although T cell proliferative and cytokine (IFN-γ and IL-4) responses to alloantigen stimulation and Con A were significantly reduced in post-G-CSF NAC, they were restored by the removal of non-T cells from post-G-CSF NAC. Furthermore, there was less T cell alloreactivity in MLR in the presence of autologous post-G-CSF monocytes than in the presence of pre-G-CSF monocytes, This alteration was not replicated in vitro by culturing PBMC with G-CSF. These results suggest that G-CSF administration suppresses T-cell proliferative and cytokine (IFN-γ and IL-4) responses to allogeneic stimulation by indirectly modulating monocyte function.

Original languageEnglish
Pages (from-to)1035-1040
Number of pages6
JournalBone Marrow Transplantation
Volume25
Issue number10
Publication statusPublished - 2000

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Granulocyte Colony-Stimulating Factor
Interleukin-4
Monocytes
T-Lymphocytes
Cytokines
Isoantigens
Homologous Transplantation

Keywords

  • G-CSF
  • IFN-γ
  • IL-4
  • PBSCT
  • T cells

ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

G-CSF reduces IFN-γ and IL-4 production by T cells after allogeneic stimulation by indirectly modulating monocyte function. / Nawa, Y.; Teshima, T.; Sunami, K.; Hiramatsu, Y.; Maeda, Yoshinobu; Yano, T.; Shinagawa, K.; Ishimaru, F.; Omoto, E.; Harada, M.

In: Bone Marrow Transplantation, Vol. 25, No. 10, 2000, p. 1035-1040.

Research output: Contribution to journalArticle

Nawa, Y, Teshima, T, Sunami, K, Hiramatsu, Y, Maeda, Y, Yano, T, Shinagawa, K, Ishimaru, F, Omoto, E & Harada, M 2000, 'G-CSF reduces IFN-γ and IL-4 production by T cells after allogeneic stimulation by indirectly modulating monocyte function', Bone Marrow Transplantation, vol. 25, no. 10, pp. 1035-1040.
Nawa, Y. ; Teshima, T. ; Sunami, K. ; Hiramatsu, Y. ; Maeda, Yoshinobu ; Yano, T. ; Shinagawa, K. ; Ishimaru, F. ; Omoto, E. ; Harada, M. / G-CSF reduces IFN-γ and IL-4 production by T cells after allogeneic stimulation by indirectly modulating monocyte function. In: Bone Marrow Transplantation. 2000 ; Vol. 25, No. 10. pp. 1035-1040.
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