G-CSF reduces IFN-γ and IL-4 production by T cells after allogeneic stimulation by indirectly modulating monocyte function

Y. Nawa, T. Teshima, K. Sunami, Y. Hiramatsu, Y. Maeda, T. Yano, K. Shinagawa, F. Ishimaru, E. Omoto, M. Harada

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45 Citations (Scopus)

Abstract

Despite a 10-fold increase of T cell dose, the incidence and severity of acute GVHD following allogeneic transplantation of G-CSF-mobilized PBSC is not increased compared to BMT. Experimental murine studies demonstrate that G-CSF polarizes donor T cells toward a type 2 cytokine response. To determine whether G-CSF alters T cell cytokine responses, we investigated the effects of G-CSF administration on T cell proliferative and cytokine responses to alloantigen and Con A in non-adherent PBMC (NAC) and CD3+ T cells obtained from normal individuals before and after G-CSF administration (10 μg/kg x 4 days). Although T cell proliferative and cytokine (IFN-γ and IL-4) responses to alloantigen stimulation and Con A were significantly reduced in post-G-CSF NAC, they were restored by the removal of non-T cells from post-G-CSF NAC. Furthermore, there was less T cell alloreactivity in MLR in the presence of autologous post-G-CSF monocytes than in the presence of pre-G-CSF monocytes, This alteration was not replicated in vitro by culturing PBMC with G-CSF. These results suggest that G-CSF administration suppresses T-cell proliferative and cytokine (IFN-γ and IL-4) responses to allogeneic stimulation by indirectly modulating monocyte function.

Original languageEnglish
Pages (from-to)1035-1040
Number of pages6
JournalBone Marrow Transplantation
Volume25
Issue number10
DOIs
Publication statusPublished - Jan 1 2000

Keywords

  • G-CSF
  • IFN-γ
  • IL-4
  • PBSCT
  • T cells

ASJC Scopus subject areas

  • Hematology
  • Transplantation

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    Nawa, Y., Teshima, T., Sunami, K., Hiramatsu, Y., Maeda, Y., Yano, T., Shinagawa, K., Ishimaru, F., Omoto, E., & Harada, M. (2000). G-CSF reduces IFN-γ and IL-4 production by T cells after allogeneic stimulation by indirectly modulating monocyte function. Bone Marrow Transplantation, 25(10), 1035-1040. https://doi.org/10.1038/sj.bmt.1702402