G-CSF enhances stem cell proliferation in rat hippocampus after transient middle cerebral artery occlusion

Yoshihide Sehara, Takeshi Hayashi, Kentaro Deguchi, Hanzhe Zhang, Atsushi Tsuchiya, Toru Yamashita, Violeta Lukic, Makiko Nagai, Tatsushi Kamiya, Koji Abe

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13 Citations (Scopus)


Granulocyte colony-stimulating factor (G-CSF) enhances the survival and stimulates the proliferation of neutrophil progenitors. Recently, the neurogenerative effect of G-CSF has been intensely investigated. In this study, we explored the possibility that G-CSF enhanced the cell proliferation in the rat dentate gyrus (DG) after focal cerebral ischemia, using a rat transient middle cerebral artery occlusion (tMCAO) model. At 7 days after tMCAO, the number of 5-bromodeoxyuridine (BrdU)-positive cells in the G-CSF-treated group was significantly increased compared with that in the vehicle-treated group in the ipsilateral SGZ (16.6 ± 5.5/mm2 in the vehicle-treated group versus 33.0 ± 7.2/mm2 in the G-CSF-treated group, **p <0.01) and in the ipsilateral GCL (14.2 ± 2.8/mm2 in the vehicle-treated group versus 21.0 ± 3.8/mm2 in the G-CSF-treated group, *p <0.05). This result showed the possibility of a neurogenerative role of G-CSF after tMCAO in rats.

Original languageEnglish
Pages (from-to)248-252
Number of pages5
JournalNeuroscience Letters
Issue number3
Publication statusPublished - May 18 2007



  • Colony-stimulating factor
  • Granulocyte
  • Hippocampus
  • Neurogenesis
  • Transient middle cerebral artery occlusion

ASJC Scopus subject areas

  • Neuroscience(all)

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