g-anisotropy of the S2-state manganese cluster in single crystals of cyanobacterial photosystem II studied by W-band electron paramagnetic resonance spectroscopy

Hideto Matsuoka, Ko Furukawa, Tatsuhi Kato, Hiroyuki Mino, Jian Ren Shen, Asako Kawamori

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The multiline signal from the S2-state manganese cluster in the oxygen evolving complex of photosystem II (PSII) was observed in single, crystals of a thermophilic cyanobacterium Thermosynechococcus vulcanus for the first time by W-band (94 GHz) electron paramagnetic resonance (EPR). At W-band, spectra were characterized by the g-anisotropy, which enabled the precise determination of the tensor. Distinct hyperfine splittings (hfs's) as seen in frozen solutions of PSII at X-band (9.5 GHz) were detected in most of the crystal orientations relative to the magnetic field. In some orientations, however, the hfs's disappeared due to overlapping of a large number of EPR lines from eight crystallographic symmetry-related sites of the manganese cluster within the unit cell of the crystal. Analysis of the orientation-dependent spectral features yielded the following g-tensor components: gx = 1.988, gz = 1.981, gz = 1.965. The principal values suggested an approximate axial symmetry around the Mn(III) ion in the cluster.

Original languageEnglish
Pages (from-to)13242-13247
Number of pages6
JournalJournal of Physical Chemistry B
Volume110
Issue number26
DOIs
Publication statusPublished - Jul 6 2006

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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