g-anisotropy of the S2-state manganese cluster in single crystals of cyanobacterial photosystem II studied by W-band electron paramagnetic resonance spectroscopy

Hideto Matsuoka, Ko Furukawa, Tatsuhi Kato, Hiroyuki Mino, Jian-Ren Shen, Asako Kawamori

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The multiline signal from the S2-state manganese cluster in the oxygen evolving complex of photosystem II (PSII) was observed in single, crystals of a thermophilic cyanobacterium Thermosynechococcus vulcanus for the first time by W-band (94 GHz) electron paramagnetic resonance (EPR). At W-band, spectra were characterized by the g-anisotropy, which enabled the precise determination of the tensor. Distinct hyperfine splittings (hfs's) as seen in frozen solutions of PSII at X-band (9.5 GHz) were detected in most of the crystal orientations relative to the magnetic field. In some orientations, however, the hfs's disappeared due to overlapping of a large number of EPR lines from eight crystallographic symmetry-related sites of the manganese cluster within the unit cell of the crystal. Analysis of the orientation-dependent spectral features yielded the following g-tensor components: gx = 1.988, gz = 1.981, gz = 1.965. The principal values suggested an approximate axial symmetry around the Mn(III) ion in the cluster.

Original languageEnglish
Pages (from-to)13242-13247
Number of pages6
JournalJournal of Physical Chemistry B
Volume110
Issue number26
DOIs
Publication statusPublished - Jul 6 2006

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Photosystem II Protein Complex
Manganese
Crystal orientation
Tensors
Paramagnetic resonance
manganese
electron paramagnetic resonance
Anisotropy
Single crystals
Spectroscopy
anisotropy
single crystals
tensors
spectroscopy
symmetry
Ions
Magnetic fields
Oxygen
superhigh frequencies
resonance lines

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

g-anisotropy of the S2-state manganese cluster in single crystals of cyanobacterial photosystem II studied by W-band electron paramagnetic resonance spectroscopy. / Matsuoka, Hideto; Furukawa, Ko; Kato, Tatsuhi; Mino, Hiroyuki; Shen, Jian-Ren; Kawamori, Asako.

In: Journal of Physical Chemistry B, Vol. 110, No. 26, 06.07.2006, p. 13242-13247.

Research output: Contribution to journalArticle

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AU - Furukawa, Ko

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AU - Mino, Hiroyuki

AU - Shen, Jian-Ren

AU - Kawamori, Asako

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AB - The multiline signal from the S2-state manganese cluster in the oxygen evolving complex of photosystem II (PSII) was observed in single, crystals of a thermophilic cyanobacterium Thermosynechococcus vulcanus for the first time by W-band (94 GHz) electron paramagnetic resonance (EPR). At W-band, spectra were characterized by the g-anisotropy, which enabled the precise determination of the tensor. Distinct hyperfine splittings (hfs's) as seen in frozen solutions of PSII at X-band (9.5 GHz) were detected in most of the crystal orientations relative to the magnetic field. In some orientations, however, the hfs's disappeared due to overlapping of a large number of EPR lines from eight crystallographic symmetry-related sites of the manganese cluster within the unit cell of the crystal. Analysis of the orientation-dependent spectral features yielded the following g-tensor components: gx = 1.988, gz = 1.981, gz = 1.965. The principal values suggested an approximate axial symmetry around the Mn(III) ion in the cluster.

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