Further stabilization of earthworm serine protease by chemical modification and immobilization

Nobuyoshi Nakajima, Kohji Ishihara, Manabu Sugimoto, Takashi Nakahara, Hideaki Tsuji

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Earthworm serine protease is more stable and is less affected by organic solvents and detergent than other proteases. However, it is inactivated, probably by autolysis, at 60°C or above under alkaline conditions. Further stabilization was managed by chemical modification of the enzyme with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and phenylglyoxal to protect the activity from the autolytic inactivation. Stabilization was possible also under acidic conditions, in which the stability of the enzyme was rather low, by immobilization with folded sheet mesoporous material. Thus, further stabilization of the enzyme has been achieved by chemical modification or immobilization.

Original languageEnglish
Pages (from-to)2739-2742
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume66
Issue number12
Publication statusPublished - Dec 2002

Fingerprint

Oligochaeta
Chemical modification
Serine Proteases
serine proteinases
earthworms
Immobilization
Enzymes
Stabilization
Phenylglyoxal
Ethyldimethylaminopropyl Carbodiimide
enzyme stability
Enzyme Stability
Autolysis
autolysis
enzymes
detergents
Detergents
inactivation
Mesoporous materials
Peptide Hydrolases

Keywords

  • Chemical modification
  • Earthworm
  • Immobilization
  • Serine protease
  • Stabilization

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Chemistry (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

Cite this

Further stabilization of earthworm serine protease by chemical modification and immobilization. / Nakajima, Nobuyoshi; Ishihara, Kohji; Sugimoto, Manabu; Nakahara, Takashi; Tsuji, Hideaki.

In: Bioscience, Biotechnology and Biochemistry, Vol. 66, No. 12, 12.2002, p. 2739-2742.

Research output: Contribution to journalArticle

Nakajima, Nobuyoshi ; Ishihara, Kohji ; Sugimoto, Manabu ; Nakahara, Takashi ; Tsuji, Hideaki. / Further stabilization of earthworm serine protease by chemical modification and immobilization. In: Bioscience, Biotechnology and Biochemistry. 2002 ; Vol. 66, No. 12. pp. 2739-2742.
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