Functional screening of salt tolerance genes from a halophyte Sporobolus virginicus and transcriptomic and metabolomic analysis of salt tolerant plants expressing glycine-rich RNA-binding protein

Yuichi Tada, Ryuichi Kawano, Shiho Komatsubara, Hideki Nishimura, Maki Katsuhara, Soichi Ozaki, Shin Terashima, Kentaro Yano, Chisato Endo, Muneo Sato, Mami Okamoto, Yuji Sawada, Masami Yokota Hirai, Takamitsu Kurusu

Research output: Contribution to journalArticle

Abstract

Sporobolus virginicus is a halophytic C4 grass found worldwide, from tropical to warm temperate regions. One Japanese genotype showed a salinity tolerance up to 1.5 M NaCl, a three-fold higher concentration than the salinity of sea water. To identify the key genes involved in the regulation of salt tolerance in S. virginicus, we produced 3500 independent transgenic Arabidopsis lines expressing random cDNA from S. virginicus and screened 10 lines which showed enhanced salt tolerance compared with the wild type in a medium containing 150 mM NaCl. Among the selected lines, two contained cDNA coding glycine-rich RNA-binding proteins (SvGRP1 and SvGRP2). This is the first reports on the function of GRPs from halophytes in salt tolerance though reports have shown GRPs are involved in diverse biological and biochemical processes including salt tolerance in Arabidopsis and some other glycophytes. Transcriptomic analysis and GO enrichment analysis of SvGRP1-expressing Arabidopsis under salt stress revealed upregulation of polyol and downregulation of glucosinolate and indole acetic acid biosynthesis/metabolic pathways. Metabolomic analysis of the SvGRP1-transformant suggested that the increase in 3-aminoppropanoic acid, citramalic acid, and isocitric acid content was associated with enhanced salt tolerance. These findings could provide novel insight into the roles of GRPs in plant salt tolerance.

LanguageEnglish
Pages54-63
Number of pages10
JournalPlant Science
Volume278
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Salt-Tolerant Plants
Sporobolus virginicus
Salt-Tolerance
RNA-binding proteins
Metabolomics
metabolomics
transcriptomics
salt tolerance
glycine (amino acid)
screening
salts
Arabidopsis
Genes
genes
Salinity
isocitric acid
Biochemical Phenomena
Complementary DNA
salinity
Glucosinolates

Keywords

  • Arabidopsis
  • cDNA expression library
  • Functional screening
  • Glycine-rich RNA-binding protein
  • Salt tolerance
  • Sporobolus virginicus

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Cite this

Functional screening of salt tolerance genes from a halophyte Sporobolus virginicus and transcriptomic and metabolomic analysis of salt tolerant plants expressing glycine-rich RNA-binding protein. / Tada, Yuichi; Kawano, Ryuichi; Komatsubara, Shiho; Nishimura, Hideki; Katsuhara, Maki; Ozaki, Soichi; Terashima, Shin; Yano, Kentaro; Endo, Chisato; Sato, Muneo; Okamoto, Mami; Sawada, Yuji; Hirai, Masami Yokota; Kurusu, Takamitsu.

In: Plant Science, Vol. 278, 01.01.2019, p. 54-63.

Research output: Contribution to journalArticle

Tada, Yuichi ; Kawano, Ryuichi ; Komatsubara, Shiho ; Nishimura, Hideki ; Katsuhara, Maki ; Ozaki, Soichi ; Terashima, Shin ; Yano, Kentaro ; Endo, Chisato ; Sato, Muneo ; Okamoto, Mami ; Sawada, Yuji ; Hirai, Masami Yokota ; Kurusu, Takamitsu. / Functional screening of salt tolerance genes from a halophyte Sporobolus virginicus and transcriptomic and metabolomic analysis of salt tolerant plants expressing glycine-rich RNA-binding protein. In: Plant Science. 2019 ; Vol. 278. pp. 54-63.
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