Abstract
The immunological manifestation of granuloma formations in humans largely depends on the delayed-type hypersensitivity response. We investigated the involvement of monocyte chemoattractant protein-1 (MCP-1) in a rabbit model of T cell-mediated pulmonary granulomatosis. Intravenous injection of Propionibacterium acnes (P. acnes) into sensitized rabbits induced massive and diffuse pulmonary granulomas. Levels of MCP-1 in sera and bronchoalveolar lavage fluids (BALF) peaked before the granuloma formation reached the peak (on days 1 and 3 after challenge, respectively). Chemotactic activities toward monocytes and T cells in BALF were inhibited by anti-MCP-1 IgG by 80 and 36%, respectively. The phenotypic analysis of the migrating T cells revealed that activated and memory T cells rather than naive cells were preferentially attracted to the BALF. Administration of anti-MCP-1 antiserum inhibited the development of granuloma formation in both size and number, the numbers of infiltrating leukocytes in BALF, the expression of adhesion molecules on peripheral monocytes/T cells, and on macrophages/T cells in BALF, and the production of TNF-α in the lung. Anti-MCP-1 resulted in a trend toward decreased level of IL-1β in the lung. The inhibition of the production of these cytokines appeared to be induced indirectly through the inhibition of the recruitment of macrophages that produce these cytokines. The results suggest important roles of MCP-1 in the development of granuloma formation in this model through the attraction and activation of specific types of cells.
| Original language | English |
|---|---|
| Pages (from-to) | 482-491 |
| Number of pages | 10 |
| Journal | Journal of Leukocyte Biology |
| Volume | 65 |
| Issue number | 4 |
| Publication status | Published - 1999 |
| Externally published | Yes |
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Keywords
- Adhesion molecules
- Chemotaxis
- Granuloma formation
- Pro-inflammatory cytokines
ASJC Scopus subject areas
- Cell Biology
Cite this
Functional roles of MCP-1 in Propionibacterium acnes-induced, T cell- mediated pulmonary granulomatosis in rabbits. / Ichiyasu, Hidenori; Suga, Moritaka; Matsukawa, Akihiro; Iyonaga, Kazuhiro; Mizobe, Takanori; Takahashi, Toshihiro; Ando, Masayuki.
In: Journal of Leukocyte Biology, Vol. 65, No. 4, 1999, p. 482-491.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional roles of MCP-1 in Propionibacterium acnes-induced, T cell- mediated pulmonary granulomatosis in rabbits
AU - Ichiyasu, Hidenori
AU - Suga, Moritaka
AU - Matsukawa, Akihiro
AU - Iyonaga, Kazuhiro
AU - Mizobe, Takanori
AU - Takahashi, Toshihiro
AU - Ando, Masayuki
PY - 1999
Y1 - 1999
N2 - The immunological manifestation of granuloma formations in humans largely depends on the delayed-type hypersensitivity response. We investigated the involvement of monocyte chemoattractant protein-1 (MCP-1) in a rabbit model of T cell-mediated pulmonary granulomatosis. Intravenous injection of Propionibacterium acnes (P. acnes) into sensitized rabbits induced massive and diffuse pulmonary granulomas. Levels of MCP-1 in sera and bronchoalveolar lavage fluids (BALF) peaked before the granuloma formation reached the peak (on days 1 and 3 after challenge, respectively). Chemotactic activities toward monocytes and T cells in BALF were inhibited by anti-MCP-1 IgG by 80 and 36%, respectively. The phenotypic analysis of the migrating T cells revealed that activated and memory T cells rather than naive cells were preferentially attracted to the BALF. Administration of anti-MCP-1 antiserum inhibited the development of granuloma formation in both size and number, the numbers of infiltrating leukocytes in BALF, the expression of adhesion molecules on peripheral monocytes/T cells, and on macrophages/T cells in BALF, and the production of TNF-α in the lung. Anti-MCP-1 resulted in a trend toward decreased level of IL-1β in the lung. The inhibition of the production of these cytokines appeared to be induced indirectly through the inhibition of the recruitment of macrophages that produce these cytokines. The results suggest important roles of MCP-1 in the development of granuloma formation in this model through the attraction and activation of specific types of cells.
AB - The immunological manifestation of granuloma formations in humans largely depends on the delayed-type hypersensitivity response. We investigated the involvement of monocyte chemoattractant protein-1 (MCP-1) in a rabbit model of T cell-mediated pulmonary granulomatosis. Intravenous injection of Propionibacterium acnes (P. acnes) into sensitized rabbits induced massive and diffuse pulmonary granulomas. Levels of MCP-1 in sera and bronchoalveolar lavage fluids (BALF) peaked before the granuloma formation reached the peak (on days 1 and 3 after challenge, respectively). Chemotactic activities toward monocytes and T cells in BALF were inhibited by anti-MCP-1 IgG by 80 and 36%, respectively. The phenotypic analysis of the migrating T cells revealed that activated and memory T cells rather than naive cells were preferentially attracted to the BALF. Administration of anti-MCP-1 antiserum inhibited the development of granuloma formation in both size and number, the numbers of infiltrating leukocytes in BALF, the expression of adhesion molecules on peripheral monocytes/T cells, and on macrophages/T cells in BALF, and the production of TNF-α in the lung. Anti-MCP-1 resulted in a trend toward decreased level of IL-1β in the lung. The inhibition of the production of these cytokines appeared to be induced indirectly through the inhibition of the recruitment of macrophages that produce these cytokines. The results suggest important roles of MCP-1 in the development of granuloma formation in this model through the attraction and activation of specific types of cells.
KW - Adhesion molecules
KW - Chemotaxis
KW - Granuloma formation
KW - Pro-inflammatory cytokines
UR - http://www.scopus.com/inward/record.url?scp=0032926847&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032926847&partnerID=8YFLogxK
M3 - Article
C2 - 10204577
AN - SCOPUS:0032926847
VL - 65
SP - 482
EP - 491
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
SN - 0741-5400
IS - 4
ER -