Functional roles of MCP-1 in Propionibacterium acnes-induced, T cell- mediated pulmonary granulomatosis in rabbits

Hidenori Ichiyasu, Moritaka Suga, Akihiro Matsukawa, Kazuhiro Iyonaga, Takanori Mizobe, Toshihiro Takahashi, Masayuki Ando

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The immunological manifestation of granuloma formations in humans largely depends on the delayed-type hypersensitivity response. We investigated the involvement of monocyte chemoattractant protein-1 (MCP-1) in a rabbit model of T cell-mediated pulmonary granulomatosis. Intravenous injection of Propionibacterium acnes (P. acnes) into sensitized rabbits induced massive and diffuse pulmonary granulomas. Levels of MCP-1 in sera and bronchoalveolar lavage fluids (BALF) peaked before the granuloma formation reached the peak (on days 1 and 3 after challenge, respectively). Chemotactic activities toward monocytes and T cells in BALF were inhibited by anti-MCP-1 IgG by 80 and 36%, respectively. The phenotypic analysis of the migrating T cells revealed that activated and memory T cells rather than naive cells were preferentially attracted to the BALF. Administration of anti-MCP-1 antiserum inhibited the development of granuloma formation in both size and number, the numbers of infiltrating leukocytes in BALF, the expression of adhesion molecules on peripheral monocytes/T cells, and on macrophages/T cells in BALF, and the production of TNF-α in the lung. Anti-MCP-1 resulted in a trend toward decreased level of IL-1β in the lung. The inhibition of the production of these cytokines appeared to be induced indirectly through the inhibition of the recruitment of macrophages that produce these cytokines. The results suggest important roles of MCP-1 in the development of granuloma formation in this model through the attraction and activation of specific types of cells.

Original languageEnglish
Pages (from-to)482-491
Number of pages10
JournalJournal of Leukocyte Biology
Volume65
Issue number4
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Propionibacterium acnes
Chemokine CCL2
Bronchoalveolar Lavage Fluid
Granuloma
Rabbits
T-Lymphocytes
Lung
Monocytes
Macrophages
Cytokines
Delayed Hypersensitivity
Interleukin-1
Leukocyte Count
Intravenous Injections
Immune Sera
Immunoglobulin G
Serum

Keywords

  • Adhesion molecules
  • Chemotaxis
  • Granuloma formation
  • Pro-inflammatory cytokines

ASJC Scopus subject areas

  • Cell Biology

Cite this

Functional roles of MCP-1 in Propionibacterium acnes-induced, T cell- mediated pulmonary granulomatosis in rabbits. / Ichiyasu, Hidenori; Suga, Moritaka; Matsukawa, Akihiro; Iyonaga, Kazuhiro; Mizobe, Takanori; Takahashi, Toshihiro; Ando, Masayuki.

In: Journal of Leukocyte Biology, Vol. 65, No. 4, 1999, p. 482-491.

Research output: Contribution to journalArticle

Ichiyasu, H, Suga, M, Matsukawa, A, Iyonaga, K, Mizobe, T, Takahashi, T & Ando, M 1999, 'Functional roles of MCP-1 in Propionibacterium acnes-induced, T cell- mediated pulmonary granulomatosis in rabbits', Journal of Leukocyte Biology, vol. 65, no. 4, pp. 482-491.
Ichiyasu, Hidenori ; Suga, Moritaka ; Matsukawa, Akihiro ; Iyonaga, Kazuhiro ; Mizobe, Takanori ; Takahashi, Toshihiro ; Ando, Masayuki. / Functional roles of MCP-1 in Propionibacterium acnes-induced, T cell- mediated pulmonary granulomatosis in rabbits. In: Journal of Leukocyte Biology. 1999 ; Vol. 65, No. 4. pp. 482-491.
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