Functional partnership between amphiphysin and dynamin in clathrin-mediated endocytosis

Kohji Takei, Vladimir I. Slepnev, Volker Haucke, Pietro De Camilli

Research output: Contribution to journalArticle

464 Citations (Scopus)

Abstract

Amphiphysin, a protein that is highly concentrated in nerve terminals, has been proposed to function as a linker between the clathrin coat and dynamin in the endocytosis of synaptic vesicles. Here, using a cell-free system, we provide direct morphological evidence in support of this hypothesis. Unexpectedly, we also find that amphiphysin-1, like dynamin-1, can transform spherical liposomes into narrow tubules. Moreover, amphiphysin-1 assembles with dynamin-1 into ring-like structures around the tubules and enhances the liposome-fragmenting activity of dynamin-1 in the presence of GTP. These results show that amphiphysin binds lipid bilayers, indicate a potential function for amphiphysin in the changes in bilayer curvature that accompany vesicle budding, and imply a close functional partnership between amphiphysin and dynamin in endocytosis.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalNature Cell Biology
Volume1
Issue number1
Publication statusPublished - May 1999
Externally publishedYes

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Dynamins
Clathrin
Endocytosis
Dynamin I
Liposomes
Cell-Free System
Synaptic Vesicles
Lipid Bilayers
Guanosine Triphosphate
amphiphysin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Functional partnership between amphiphysin and dynamin in clathrin-mediated endocytosis. / Takei, Kohji; Slepnev, Vladimir I.; Haucke, Volker; De Camilli, Pietro.

In: Nature Cell Biology, Vol. 1, No. 1, 05.1999, p. 33-39.

Research output: Contribution to journalArticle

Takei, K, Slepnev, VI, Haucke, V & De Camilli, P 1999, 'Functional partnership between amphiphysin and dynamin in clathrin-mediated endocytosis', Nature Cell Biology, vol. 1, no. 1, pp. 33-39.
Takei, Kohji ; Slepnev, Vladimir I. ; Haucke, Volker ; De Camilli, Pietro. / Functional partnership between amphiphysin and dynamin in clathrin-mediated endocytosis. In: Nature Cell Biology. 1999 ; Vol. 1, No. 1. pp. 33-39.
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