Functional mutation of DNA polymerase β found in human gastric cancer - Inability of the base excision repair in vitro

Akinari Iwanaga, Mamoru Ouchida, Kohji Miyazaki, Katsuji Hori, Tsunehiro Mukai

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

DNA polymerase β (polβ) is one of mammalian DNA polymerases and is known to be involved in a G:T/G:U mismatch repair. In order to investigate an involvement of this enzyme in a base excision repair, we searched a mutation of human polβ in human gastric cancer and studied a function of the mutation. We observed cancer-specific missense mutations in 6 of 20 samples. All of these mutations were, however, heterozygous. We further analyzed the base excision repair activity of these mutants to know whether these mutants cause an error of mismatch repair. One of these mutants, which resulted in an amino acid substitution of Glu for Lys at codon 295, showed an inhibitory effect by in vitro base excision repair assay, suggesting that this mutation might play some role in carcinogenesis of the gastric mucosa.

Original languageEnglish
Pages (from-to)121-128
Number of pages8
JournalMutation Research - DNA Repair
Volume435
Issue number2
DOIs
Publication statusPublished - Oct 22 1999

ASJC Scopus subject areas

  • Molecular Biology
  • Toxicology
  • Genetics

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