Functional gene cloning and characterization of the SsmE multidrug efflux pump from Serratia marcescens

Yusuke Minato, Fereshteh Shahcheraghi, Wakano Ogawa, Teruo Kuroda, Tomofusa Tsuchiya

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22 Citations (Scopus)

Abstract

We cloned a gene responsible for multidrug resistance from chromosomal DNA of Serratia marcescens, and determined the nucleotide sequence. We designated the gene as ssmE. The deduced amino acid sequence of SsmE showed high similarity with the small multidrug resistance (SMR)-type multidrug efflux pumps. Cells of Escherichia coli KAM32, a drug hyper-susceptible mutant, transformed with a plasmid pESM437 carrying the ssmE gene showed elevated minimum inhibitory concentrations of structurally unrelated antimicrobial agents. E. coli KAM32/pESM437 showed elevated energy dependent efflux of ethidium. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis revealed that ssmE was expressed in cells of S. marcescens.

Original languageEnglish
Pages (from-to)516-519
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume31
Issue number3
DOIs
Publication statusPublished - Mar 2008

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Keywords

  • Drug resistance
  • Multidrug efflux pump
  • Serratia marcescens
  • SsmE

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

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