Functional expression of metabotropic glutamate receptor type 5 in rat pinealocytes

Shouki Yatsushiro, Hiroshi Yamada, Mitsuko Hayashi, Seiji Tsuboi, Yoshinori Moriyama

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Mammalian pinealocytes, endocrine cells for melatonin, express class II metabotropic glutamate receptors (mGluRs), which are involved in negative regulation of melatonin synthesis through an inhibitory cAMP cascade. We investigated whether mGluRs other than class II receptors are expressed in rat pinealocytes. RT-PCR analysis and Northern blotting indicated that the mRNA of mGluR5, a class I receptor, was present in pineal glands. Quisqualate and 1-aminocyclopentane-1,3-dicarboxylate (1S,3R-ACPD), class I receptor agonists, increased the intracellular [Ca2+] of fura-2 loaded cultured pinealocytes in the absence of extracellular Ca2+ which is blocked by methylcarboxyphenylglycine, a class I receptor antagonist. These results suggest that mGluR5 is functionally expressed in pinealocytes and triggers Ca2+ efflux from intracellular stores.

Original languageEnglish
Pages (from-to)1599-1603
Number of pages5
Issue number7
Publication statusPublished - May 14 1999


  • Endocrine cell
  • Glutamate
  • Metabotropic glutamate receptor type 5
  • Pinealocyte

ASJC Scopus subject areas

  • Neuroscience(all)


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