TY - JOUR
T1 - Functional expression of metabotropic glutamate receptor type 5 in rat pinealocytes
AU - Yatsushiro, Shouki
AU - Yamada, Hiroshi
AU - Hayashi, Mitsuko
AU - Tsuboi, Seiji
AU - Moriyama, Yoshinori
PY - 1999/5/14
Y1 - 1999/5/14
N2 - Mammalian pinealocytes, endocrine cells for melatonin, express class II metabotropic glutamate receptors (mGluRs), which are involved in negative regulation of melatonin synthesis through an inhibitory cAMP cascade. We investigated whether mGluRs other than class II receptors are expressed in rat pinealocytes. RT-PCR analysis and Northern blotting indicated that the mRNA of mGluR5, a class I receptor, was present in pineal glands. Quisqualate and 1-aminocyclopentane-1,3-dicarboxylate (1S,3R-ACPD), class I receptor agonists, increased the intracellular [Ca2+] of fura-2 loaded cultured pinealocytes in the absence of extracellular Ca2+ which is blocked by methylcarboxyphenylglycine, a class I receptor antagonist. These results suggest that mGluR5 is functionally expressed in pinealocytes and triggers Ca2+ efflux from intracellular stores.
AB - Mammalian pinealocytes, endocrine cells for melatonin, express class II metabotropic glutamate receptors (mGluRs), which are involved in negative regulation of melatonin synthesis through an inhibitory cAMP cascade. We investigated whether mGluRs other than class II receptors are expressed in rat pinealocytes. RT-PCR analysis and Northern blotting indicated that the mRNA of mGluR5, a class I receptor, was present in pineal glands. Quisqualate and 1-aminocyclopentane-1,3-dicarboxylate (1S,3R-ACPD), class I receptor agonists, increased the intracellular [Ca2+] of fura-2 loaded cultured pinealocytes in the absence of extracellular Ca2+ which is blocked by methylcarboxyphenylglycine, a class I receptor antagonist. These results suggest that mGluR5 is functionally expressed in pinealocytes and triggers Ca2+ efflux from intracellular stores.
KW - Endocrine cell
KW - Glutamate
KW - Metabotropic glutamate receptor type 5
KW - Pinealocyte
UR - http://www.scopus.com/inward/record.url?scp=0033553351&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033553351&partnerID=8YFLogxK
U2 - 10.1097/00001756-199905140-00038
DO - 10.1097/00001756-199905140-00038
M3 - Article
C2 - 10380988
AN - SCOPUS:0033553351
VL - 10
SP - 1599
EP - 1603
JO - NeuroReport
JF - NeuroReport
SN - 0959-4965
IS - 7
ER -