Functional expression of Fas and Fas ligand on human intestinal intraepithelial lymphocytes

Y. Morimoto, A. Hizuta, E. X. Ding, T. Ishii, T. Hongo, Toshiyoshi Fujiwara, H. Iwagaki, N. Tanaka

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Intestinal intraepithelial lymphocytes (IEL) constitute the first lymphoid compartment to encounter dietary antigens and intestinal pathogens. IEL are proposed to be involved in the defence against bacterial and vital invasion and to play an important role in mucosal immunity. Fas (CD95/APO-1) is a surface receptor that induces apoptotic cell death upon ligation with Fas ligand (FasL). The aim of this study was to examine the expression and function of Fas and FasL on freshly isolated normal human colonic IEL. The expression and function of Fas and FasL on IEL isolated from 40 normal colonic specimens were examined by flow cytometry, reverse transcriptase- polymerase chain reaction, immunohistochemistry, and DNA-release cytotoxicity assay. Virtually all CD3+ IEL (95.2 ± 4.3%) expressed Fas and were sensitive to agonistic anti-Fas antibody, whereas only 56.6 ± 8.4% of peripheral T lymphocytes expressed Fas and were resistant to the antibody. We also detected FasL mRNA and protein (40.1 ± 4.2%) on IEL, and found that IEL exerted FasL-mediated cytotoxicity against Fas-expressing target cells. These findings suggest that human IEL are activated in situ but are tightly regulated by the constitutive expression of functional Fas and FasL to maintain homeostasis of the mucosal immune system.

Original languageEnglish
Pages (from-to)84-89
Number of pages6
JournalClinical and Experimental Immunology
Volume116
Issue number1
DOIs
Publication statusPublished - 1999

Fingerprint

Fas Ligand Protein
Lymphocytes
Mucosal Immunity
Reverse Transcriptase Polymerase Chain Reaction
Ligation
Anti-Idiotypic Antibodies
Immune System
Flow Cytometry
Homeostasis
Cell Death
Immunohistochemistry
T-Lymphocytes
Antigens
Messenger RNA
Antibodies
DNA

Keywords

  • Apoptosis
  • Fas
  • Fas ligand
  • Intestinal intraepithelial lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Functional expression of Fas and Fas ligand on human intestinal intraepithelial lymphocytes. / Morimoto, Y.; Hizuta, A.; Ding, E. X.; Ishii, T.; Hongo, T.; Fujiwara, Toshiyoshi; Iwagaki, H.; Tanaka, N.

In: Clinical and Experimental Immunology, Vol. 116, No. 1, 1999, p. 84-89.

Research output: Contribution to journalArticle

Morimoto, Y. ; Hizuta, A. ; Ding, E. X. ; Ishii, T. ; Hongo, T. ; Fujiwara, Toshiyoshi ; Iwagaki, H. ; Tanaka, N. / Functional expression of Fas and Fas ligand on human intestinal intraepithelial lymphocytes. In: Clinical and Experimental Immunology. 1999 ; Vol. 116, No. 1. pp. 84-89.
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