Functional domains of the human B-myb gene product

Hideki Nakagoshi, Yoshihiro Takemoto, Shunsuke Ishii

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Three members of the human myb gene family (c-myb, A-myb, and B-myb) encode transcriptional regulators that can bind to specific DNA sequences. High levels of c-myb expression are usually found in immature hemopoietic cells, but the B-myb is more commonly expressed in many types of cells. To understand the regulation of the activity of B-myb gene product (B-Myb), its functional domains were analyzed. Like c-Myb, B-Myb also has a transcriptional activation domain containing a cluster of acidic amino acids in the region downstream of the DNA-binding domain, which consists of three tandem repeats of 51-52 amino acids. In contrast to c-Myb, B-Myb does not contain a negative regulatory domain. Furthermore, the multiple nuclear localization signals are in at least two regions in the COOH-terminal half of B-Myb, and one of them is adjacent to a potential cdc2 kinase site. These results indicate that B-Myb contains DNA-binding and transcriptional activation domains similar to those of c-Myb, but a regulatory mechanism of B-Myb activity is quite different from that for c-Myb.

Original languageEnglish
Pages (from-to)14161-14167
Number of pages7
JournalJournal of Biological Chemistry
Volume268
Issue number19
Publication statusPublished - Jul 5 1993
Externally publishedYes

Fingerprint

myb Genes
Genes
Chemical activation
Acidic Amino Acids
Transcriptional Activation
Tandem Repeat Sequences
DNA sequences
DNA
Nuclear Localization Signals
B-Lymphoid Precursor Cells
Phosphotransferases
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Functional domains of the human B-myb gene product. / Nakagoshi, Hideki; Takemoto, Yoshihiro; Ishii, Shunsuke.

In: Journal of Biological Chemistry, Vol. 268, No. 19, 05.07.1993, p. 14161-14167.

Research output: Contribution to journalArticle

Nakagoshi, H, Takemoto, Y & Ishii, S 1993, 'Functional domains of the human B-myb gene product', Journal of Biological Chemistry, vol. 268, no. 19, pp. 14161-14167.
Nakagoshi, Hideki ; Takemoto, Yoshihiro ; Ishii, Shunsuke. / Functional domains of the human B-myb gene product. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 19. pp. 14161-14167.
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