Functional cloning of the cDNA for a human hyaluronan synthase

Anne M. Shyjan, Paraskevi Heldin, Eugene C. Butcher, Tadashi Yoshino, Michael J. Briskin

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

Hyaluronan is a constituent of the extracellular matrix of connective tissue and is actively synthesized during wound healing and tissue repair to provide a frame-work for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of hyaluronan are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, hyaluronan has been implicated as an important substrate for migration of adhesion of leukocytes during inflammation. A human hyaluronan synthase (HuHAS1) cDNA was isolated by a functional expression cloning approach. Transfection of CHO cells conferred hyaluronidase-sensitive adhesiveness of a mucosal T cell line via the lymphocyte hyaluronan receptor, CD44, as well as increased hyaluronan levels in the cultures of transfected cells. The HuHAS1 amino acid sequence shows considerable homology to the hasA gene product of Streptococcus pyogenes, a glycosaminoglycan synthetase from Xenopus laevis (DG42), and is the human homolog of a recently described murine hyaluronan synthase.

Original languageEnglish
Pages (from-to)23395-23399
Number of pages5
JournalJournal of Biological Chemistry
Volume271
Issue number38
DOIs
Publication statusPublished - Sep 30 1996

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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