TY - JOUR
T1 - Functional cloning of a cDNA encoding Mei2-like protein from Arabidopsis thaliana using a fission yeast pheromone receptor deficient mutant
AU - Hirayama, Takashi
AU - Ishida, Chika
AU - Kuromori, Takashi
AU - Obata, Shusei
AU - Shimoda, Chikashi
AU - Yamamoto, Masayuki
AU - Shinozaki, Kazuo
AU - Ohto, Chikara
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1997/8/11
Y1 - 1997/8/11
N2 - To isolate Arabidopsis cDNAs that encode signal transducers and components involved in the regulation of meiosis, a trans-complementation analysis was performed using a Schizosaccharomyces pombe meiosis-defective mutant in which the genes for pheromone receptors were disabled. One cDNA obtained in this screening encodes a polypeptide, named AML1, that shows significant similarity to S. pombe Mei2 protein and has three putative RNA-recognition motifs like as Mei2. Mei2 is involved in the regulation of meiosis in fission yeast. Northern blot analysis showed that the AML1 gene is expressed in each organ. The possible functions of AML1 are discussed.
AB - To isolate Arabidopsis cDNAs that encode signal transducers and components involved in the regulation of meiosis, a trans-complementation analysis was performed using a Schizosaccharomyces pombe meiosis-defective mutant in which the genes for pheromone receptors were disabled. One cDNA obtained in this screening encodes a polypeptide, named AML1, that shows significant similarity to S. pombe Mei2 protein and has three putative RNA-recognition motifs like as Mei2. Mei2 is involved in the regulation of meiosis in fission yeast. Northern blot analysis showed that the AML1 gene is expressed in each organ. The possible functions of AML1 are discussed.
KW - Arabidopsis thaliana
KW - Meiosis induction
KW - RNA-recognition motif
KW - Schizosaccharomyces pombe
KW - Trans-complementation
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U2 - 10.1016/S0014-5793(97)00871-5
DO - 10.1016/S0014-5793(97)00871-5
M3 - Article
C2 - 9287109
AN - SCOPUS:0030801119
VL - 413
SP - 16
EP - 20
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -