Functional cloning of a cDNA encoding Mei2-like protein from Arabidopsis thaliana using a fission yeast pheromone receptor deficient mutant

Takashi Hirayama, Chika Ishida, Takashi Kuromori, Shusei Obata, Chikashi Shimoda, Masayuki Yamamoto, Kazuo Shinozaki, Chikara Ohto

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

To isolate Arabidopsis cDNAs that encode signal transducers and components involved in the regulation of meiosis, a trans-complementation analysis was performed using a Schizosaccharomyces pombe meiosis-defective mutant in which the genes for pheromone receptors were disabled. One cDNA obtained in this screening encodes a polypeptide, named AML1, that shows significant similarity to S. pombe Mei2 protein and has three putative RNA-recognition motifs like as Mei2. Mei2 is involved in the regulation of meiosis in fission yeast. Northern blot analysis showed that the AML1 gene is expressed in each organ. The possible functions of AML1 are discussed.

Original languageEnglish
Pages (from-to)16-20
Number of pages5
JournalFEBS Letters
Volume413
Issue number1
DOIs
Publication statusPublished - Aug 11 1997
Externally publishedYes

Keywords

  • Arabidopsis thaliana
  • Meiosis induction
  • RNA-recognition motif
  • Schizosaccharomyces pombe
  • Trans-complementation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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