Functional characterization of Synechocystis sp. PCC 6803 δpsbU and δpsbV mutants reveals important roles of cytochrome c-550 in cyanobacterial oxygen evolution

Jian-Ren Shen, Ming Qian, Yorinao Inoue, Robert L. Burnap

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

The functions of cytochrome c-550 and a 12 kDa protein in cyanobacterial oxygen evolution were studied with directed deletion mutants ΔpsbV and ΔpsbU of Synechocystis sp. PCC 6803, and the following results were obtained. (1) In contrast to the ΔpsbU mutant which is capable of autotrophic growth in the absence of Ca2+ or Cl- at a reduced rate, the ΔpsbV mutant lacking cytochrome c-550 could not grow at all without Ca2+ or Cl-. (2) The ΔpsbV mutant had a significantly reduced thermoluminescence emission intensity and flash oxygen yield, whereas the ΔpsbU mutant showed slight decreases in thermoluminescence intensity and flash oxygen yield, indicating corresponding decreases in the concentrations of O2-evolving centers in these mutants. (3) The ΔpsbV and ΔpsbU mutants exhibited elevated peak temperature for the thermoluminescence B- and Q-bands indicative of more stable S2 states. (4) The rise time of the O2 signal during the S3-[S4]-S0 transition was increased slightly in the ΔpsbV mutant but not in the ΔpsbU mutant. (5) The oxygen evolution was inactivated in the dark rapidly in the ΔpsbV mutant with a half-time of 28 min, but this did not happen in the ΔpsbU mutant. (6) Photoactivation of the oxygen- evolving complex after removal of the manganese cluster by hydroxylamine showed a higher quantum yield in the ΔpsbV mutant than in the ΔpsbU mutant or wild type. Taken together, these results indicated that cytochrome c-550 plays a substantial role in maintaining the stability and function of the manganese cluster in algal photosystem II, whereas the 12 kDa protein plays primarily a regulatory role in maintaining normal S-state transitions. These functional features of cytochrome c-550 and the 12 kDa protein were compared with those of the 23 and 17 kDa proteins in higher plant photosystem II and of the 33 kDa protein in both algal and plant photosystem II.

Original languageEnglish
Pages (from-to)1551-1558
Number of pages8
JournalBiochemistry
Volume37
Issue number6
DOIs
Publication statusPublished - Feb 10 1998
Externally publishedYes

Fingerprint

Synechocystis
Cytochromes c
Thermoluminescence
Photosystem II Protein Complex
Oxygen
Manganese
Proteins
Autotrophic Processes
Cytochromes a
Hydroxylamine
Quantum yield
Temperature

ASJC Scopus subject areas

  • Biochemistry

Cite this

Functional characterization of Synechocystis sp. PCC 6803 δpsbU and δpsbV mutants reveals important roles of cytochrome c-550 in cyanobacterial oxygen evolution. / Shen, Jian-Ren; Qian, Ming; Inoue, Yorinao; Burnap, Robert L.

In: Biochemistry, Vol. 37, No. 6, 10.02.1998, p. 1551-1558.

Research output: Contribution to journalArticle

@article{cc005c36b19342f98a2d533bc86837f7,
title = "Functional characterization of Synechocystis sp. PCC 6803 δpsbU and δpsbV mutants reveals important roles of cytochrome c-550 in cyanobacterial oxygen evolution",
abstract = "The functions of cytochrome c-550 and a 12 kDa protein in cyanobacterial oxygen evolution were studied with directed deletion mutants ΔpsbV and ΔpsbU of Synechocystis sp. PCC 6803, and the following results were obtained. (1) In contrast to the ΔpsbU mutant which is capable of autotrophic growth in the absence of Ca2+ or Cl- at a reduced rate, the ΔpsbV mutant lacking cytochrome c-550 could not grow at all without Ca2+ or Cl-. (2) The ΔpsbV mutant had a significantly reduced thermoluminescence emission intensity and flash oxygen yield, whereas the ΔpsbU mutant showed slight decreases in thermoluminescence intensity and flash oxygen yield, indicating corresponding decreases in the concentrations of O2-evolving centers in these mutants. (3) The ΔpsbV and ΔpsbU mutants exhibited elevated peak temperature for the thermoluminescence B- and Q-bands indicative of more stable S2 states. (4) The rise time of the O2 signal during the S3-[S4]-S0 transition was increased slightly in the ΔpsbV mutant but not in the ΔpsbU mutant. (5) The oxygen evolution was inactivated in the dark rapidly in the ΔpsbV mutant with a half-time of 28 min, but this did not happen in the ΔpsbU mutant. (6) Photoactivation of the oxygen- evolving complex after removal of the manganese cluster by hydroxylamine showed a higher quantum yield in the ΔpsbV mutant than in the ΔpsbU mutant or wild type. Taken together, these results indicated that cytochrome c-550 plays a substantial role in maintaining the stability and function of the manganese cluster in algal photosystem II, whereas the 12 kDa protein plays primarily a regulatory role in maintaining normal S-state transitions. These functional features of cytochrome c-550 and the 12 kDa protein were compared with those of the 23 and 17 kDa proteins in higher plant photosystem II and of the 33 kDa protein in both algal and plant photosystem II.",
author = "Jian-Ren Shen and Ming Qian and Yorinao Inoue and Burnap, {Robert L.}",
year = "1998",
month = "2",
day = "10",
doi = "10.1021/bi971676i",
language = "English",
volume = "37",
pages = "1551--1558",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "6",

}

TY - JOUR

T1 - Functional characterization of Synechocystis sp. PCC 6803 δpsbU and δpsbV mutants reveals important roles of cytochrome c-550 in cyanobacterial oxygen evolution

AU - Shen, Jian-Ren

AU - Qian, Ming

AU - Inoue, Yorinao

AU - Burnap, Robert L.

PY - 1998/2/10

Y1 - 1998/2/10

N2 - The functions of cytochrome c-550 and a 12 kDa protein in cyanobacterial oxygen evolution were studied with directed deletion mutants ΔpsbV and ΔpsbU of Synechocystis sp. PCC 6803, and the following results were obtained. (1) In contrast to the ΔpsbU mutant which is capable of autotrophic growth in the absence of Ca2+ or Cl- at a reduced rate, the ΔpsbV mutant lacking cytochrome c-550 could not grow at all without Ca2+ or Cl-. (2) The ΔpsbV mutant had a significantly reduced thermoluminescence emission intensity and flash oxygen yield, whereas the ΔpsbU mutant showed slight decreases in thermoluminescence intensity and flash oxygen yield, indicating corresponding decreases in the concentrations of O2-evolving centers in these mutants. (3) The ΔpsbV and ΔpsbU mutants exhibited elevated peak temperature for the thermoluminescence B- and Q-bands indicative of more stable S2 states. (4) The rise time of the O2 signal during the S3-[S4]-S0 transition was increased slightly in the ΔpsbV mutant but not in the ΔpsbU mutant. (5) The oxygen evolution was inactivated in the dark rapidly in the ΔpsbV mutant with a half-time of 28 min, but this did not happen in the ΔpsbU mutant. (6) Photoactivation of the oxygen- evolving complex after removal of the manganese cluster by hydroxylamine showed a higher quantum yield in the ΔpsbV mutant than in the ΔpsbU mutant or wild type. Taken together, these results indicated that cytochrome c-550 plays a substantial role in maintaining the stability and function of the manganese cluster in algal photosystem II, whereas the 12 kDa protein plays primarily a regulatory role in maintaining normal S-state transitions. These functional features of cytochrome c-550 and the 12 kDa protein were compared with those of the 23 and 17 kDa proteins in higher plant photosystem II and of the 33 kDa protein in both algal and plant photosystem II.

AB - The functions of cytochrome c-550 and a 12 kDa protein in cyanobacterial oxygen evolution were studied with directed deletion mutants ΔpsbV and ΔpsbU of Synechocystis sp. PCC 6803, and the following results were obtained. (1) In contrast to the ΔpsbU mutant which is capable of autotrophic growth in the absence of Ca2+ or Cl- at a reduced rate, the ΔpsbV mutant lacking cytochrome c-550 could not grow at all without Ca2+ or Cl-. (2) The ΔpsbV mutant had a significantly reduced thermoluminescence emission intensity and flash oxygen yield, whereas the ΔpsbU mutant showed slight decreases in thermoluminescence intensity and flash oxygen yield, indicating corresponding decreases in the concentrations of O2-evolving centers in these mutants. (3) The ΔpsbV and ΔpsbU mutants exhibited elevated peak temperature for the thermoluminescence B- and Q-bands indicative of more stable S2 states. (4) The rise time of the O2 signal during the S3-[S4]-S0 transition was increased slightly in the ΔpsbV mutant but not in the ΔpsbU mutant. (5) The oxygen evolution was inactivated in the dark rapidly in the ΔpsbV mutant with a half-time of 28 min, but this did not happen in the ΔpsbU mutant. (6) Photoactivation of the oxygen- evolving complex after removal of the manganese cluster by hydroxylamine showed a higher quantum yield in the ΔpsbV mutant than in the ΔpsbU mutant or wild type. Taken together, these results indicated that cytochrome c-550 plays a substantial role in maintaining the stability and function of the manganese cluster in algal photosystem II, whereas the 12 kDa protein plays primarily a regulatory role in maintaining normal S-state transitions. These functional features of cytochrome c-550 and the 12 kDa protein were compared with those of the 23 and 17 kDa proteins in higher plant photosystem II and of the 33 kDa protein in both algal and plant photosystem II.

UR - http://www.scopus.com/inward/record.url?scp=0032502005&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032502005&partnerID=8YFLogxK

U2 - 10.1021/bi971676i

DO - 10.1021/bi971676i

M3 - Article

VL - 37

SP - 1551

EP - 1558

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 6

ER -