Functional characterization of key structural genes in rice flavonoid biosynthesis

Chun Hat Shih, Hung Chu, Lee Kwan Tang, Wataru Sakamoto, Masahiko Maekawa, Ivan K. Chu, Mingfu Wang, Clive Lo

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Rice is a model system for monocot but the molecular features of rice flavonoid biosynthesis have not been extensively characterized. Rice structural gene homologs encoding chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonoid 3′-hydroxylase (F3′H), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS) were identified by homology searches. Unique differential expression of OsF3H, OsDFR, and OsANS1 controlled by the Pl w locus, which contains the R/B-type regulatory genes OSB1 and OSB2, was demonstrated during light-induced anthocyanin accumulation in T65-Plw seedlings. Previously, F3H genes were often considered as early genes co-regulated with CHS and CHI genes in other plants. In selected non-pigmented rice lines, OSB2 is not expressed following illumination while their expressed OSB1sequences all contain the same nucleotide change leading to the T64 M substitution within the conserved N-terminal interacting domain. Furthermore, the biochemical roles of the expressed rice structural genes (OsCHS1, OsCHI, OsF3H, and OsF3′H) were established in planta for the first time by complementation in the appropriate Arabidopsis transparent testa mutants. Using yeast two-hybrid analysis, OsCHS1 was demonstrated to interact physically with OsF3H, OsF3′H, OsDFR, and OsANS1, suggesting the existence of a macromolecular complex for anthocyanin biosynthesis in rice. Finally, flavones were identified as the major flavonoid class in the non-pigmented T65 seedlings in which the single-copy OsF3H gene was not expressed. Competition between flavone and anthocyanin pathways was evidenced by the significant reduction of tricin accumulation in the T65-Plw seedlings.

Original languageEnglish
Pages (from-to)1043-1054
Number of pages12
JournalPlanta
Volume228
Issue number6
DOIs
Publication statusPublished - Nov 2008

Fingerprint

structural genes
Flavonoids
flavonoids
chalcone isomerase
biosynthesis
rice
Anthocyanins
Seedlings
Genes
anthocyanins
flavone
naringenin-chalcone synthase
flavonoid 3'-hydroxylase
flavones
seedlings
genes
Macromolecular Substances
Flavones
two hybrid system techniques
anthocyanidins

Keywords

  • Anthocyanin
  • Flavones
  • Flavonoid structural genes
  • Rice

ASJC Scopus subject areas

  • Plant Science
  • Genetics

Cite this

Shih, C. H., Chu, H., Tang, L. K., Sakamoto, W., Maekawa, M., Chu, I. K., ... Lo, C. (2008). Functional characterization of key structural genes in rice flavonoid biosynthesis. Planta, 228(6), 1043-1054. https://doi.org/10.1007/s00425-008-0806-1

Functional characterization of key structural genes in rice flavonoid biosynthesis. / Shih, Chun Hat; Chu, Hung; Tang, Lee Kwan; Sakamoto, Wataru; Maekawa, Masahiko; Chu, Ivan K.; Wang, Mingfu; Lo, Clive.

In: Planta, Vol. 228, No. 6, 11.2008, p. 1043-1054.

Research output: Contribution to journalArticle

Shih, CH, Chu, H, Tang, LK, Sakamoto, W, Maekawa, M, Chu, IK, Wang, M & Lo, C 2008, 'Functional characterization of key structural genes in rice flavonoid biosynthesis', Planta, vol. 228, no. 6, pp. 1043-1054. https://doi.org/10.1007/s00425-008-0806-1
Shih, Chun Hat ; Chu, Hung ; Tang, Lee Kwan ; Sakamoto, Wataru ; Maekawa, Masahiko ; Chu, Ivan K. ; Wang, Mingfu ; Lo, Clive. / Functional characterization of key structural genes in rice flavonoid biosynthesis. In: Planta. 2008 ; Vol. 228, No. 6. pp. 1043-1054.
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