Functional characterization of CEBiP and CERK1 homologs in arabidopsis and rice reveals the presence of different chitin receptor systems in plants

Tomonori Shinya, Noriko Motoyama, Asahi Ikeda, Miyuki Wada, Kota Kamiya, Masahiro Hayafune, Hanae Kaku, Naoto Shibuya

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Chitin is a representative microbe-associated molecular pattern (MAMP) molecule for various fungi and induces immune responses in many plant species. It has been clarified that the chitin signaling in rice requires a receptor kinase OsCERK1 and a receptor-like protein (Os)CEBiP, which specifically binds chitin oligosaccharides. On the other hand, Arabidopsis requires a receptor kinase (At)CERK1 for chitin signaling but it is not clear whether the plant also requires a CEBiP-like molecule for chitin perception/signaling. To clarify the similarity/difference of the chitin receptor in these two model plants, we first characterized CEBiP homologs in Arabidopsis. Only one of three CEBiP homologs, AtCEBiP (LYM2), showed a high-affinity binding for chitin oligosaccharides similar to rice CEBiP. AtCEBiP also represented the major chitin-binding protein in the Arabidopsis membrane. However, the single/triple knockout (KO) mutants of Arabidopsis CEBiP homologs and the overexpressor of AtCEBiP showed chitin-induced defense responses similar to wild-type Arabidopsis, indicating that AtCEBiP is biochemically functional as a chitin-binding protein but does not contribute to signaling. Studies of the chitin binding properties of the ectodomains of At/OsCERK1 and the chimeric receptors consisting of ecto/cytosolic domains of these molecules indicated that AtCERK1 is sufficient for chitin perception by itself.

Original languageEnglish
Pages (from-to)1696-1706
Number of pages11
JournalPlant and Cell Physiology
Volume53
Issue number10
DOIs
Publication statusPublished - Oct 2012
Externally publishedYes

Fingerprint

Chitin
chitin
Arabidopsis
rice
receptors
Oligosaccharides
oligosaccharides
Oryza
binding proteins
Carrier Proteins
phosphotransferases (kinases)
Phosphotransferases
knockout mutants
binding properties
Fungi
immune response

Keywords

  • CEBiP
  • CERK1
  • Chitin
  • MAMP
  • Plant immunity
  • Receptor

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Functional characterization of CEBiP and CERK1 homologs in arabidopsis and rice reveals the presence of different chitin receptor systems in plants. / Shinya, Tomonori; Motoyama, Noriko; Ikeda, Asahi; Wada, Miyuki; Kamiya, Kota; Hayafune, Masahiro; Kaku, Hanae; Shibuya, Naoto.

In: Plant and Cell Physiology, Vol. 53, No. 10, 10.2012, p. 1696-1706.

Research output: Contribution to journalArticle

Shinya, Tomonori ; Motoyama, Noriko ; Ikeda, Asahi ; Wada, Miyuki ; Kamiya, Kota ; Hayafune, Masahiro ; Kaku, Hanae ; Shibuya, Naoto. / Functional characterization of CEBiP and CERK1 homologs in arabidopsis and rice reveals the presence of different chitin receptor systems in plants. In: Plant and Cell Physiology. 2012 ; Vol. 53, No. 10. pp. 1696-1706.
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