Functional characterization of an aluminum (Al)-inducible transcription factor, ART2, revealed a different pathway for Al tolerance in rice

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

High aluminum (Al) tolerance in rice (Oryza sativa) is controlled by a Cys2His2-type zinc finger transcription factor ART1 (Al resistance transcription factor 1). There are five close homologs of ART1 in the rice genome, but the role of these homologs is unknown. We functionally characterized one of the ART1 homologs, ART2, in terms of tissue and spatial expression, subcellular localization, transcriptional activation activity, and phenotypic analysis of the knockout lines. ART2 was localized to the nucleus and showed a transcriptional activation potential in yeast. ART2 was mainly expressed in the roots, but the expression level was much lower than that of ART1. The ART2 expression was rapidly induced by Al in the roots of the wild-type rice, but not in art1 mutant. Knockout of ART2 resulted in increased sensitivity to Al toxicity, but did not alter sensitivity to different pH values. Expression profile analysis by RNA-sequencing showed that ART2 was not involved in activation of genes regulated by ART1; however, four genes seems to be regulated by ART2, which are implicated in Al tolerance. These results indicate that ART1 and ART2 regulate different pathways leading to Al tolerance, and ATR2 plays a supplementary role in Al tolerance in rice.

Original languageEnglish
Pages (from-to)209-218
Number of pages10
JournalNew Phytologist
Volume220
Issue number1
DOIs
Publication statusPublished - Oct 1 2018

Fingerprint

Aluminum
aluminum
Transcription Factors
transcription factors
transcription (genetics)
rice
Transcriptional Activation
transcriptional activation
RNA Sequence Analysis
gene activation
zinc finger motif
Zinc Fingers
Oryza
Oryza sativa
sequence analysis
Yeasts
Genome
yeasts
toxicity
mutants

Keywords

  • aluminum (Al) tolerance
  • aluminum resistance transcription factor 2 (ART2)
  • regulation
  • rice
  • transcription factor

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

@article{25560aa3e7fe47ada699f23549cb71d8,
title = "Functional characterization of an aluminum (Al)-inducible transcription factor, ART2, revealed a different pathway for Al tolerance in rice",
abstract = "High aluminum (Al) tolerance in rice (Oryza sativa) is controlled by a Cys2His2-type zinc finger transcription factor ART1 (Al resistance transcription factor 1). There are five close homologs of ART1 in the rice genome, but the role of these homologs is unknown. We functionally characterized one of the ART1 homologs, ART2, in terms of tissue and spatial expression, subcellular localization, transcriptional activation activity, and phenotypic analysis of the knockout lines. ART2 was localized to the nucleus and showed a transcriptional activation potential in yeast. ART2 was mainly expressed in the roots, but the expression level was much lower than that of ART1. The ART2 expression was rapidly induced by Al in the roots of the wild-type rice, but not in art1 mutant. Knockout of ART2 resulted in increased sensitivity to Al toxicity, but did not alter sensitivity to different pH values. Expression profile analysis by RNA-sequencing showed that ART2 was not involved in activation of genes regulated by ART1; however, four genes seems to be regulated by ART2, which are implicated in Al tolerance. These results indicate that ART1 and ART2 regulate different pathways leading to Al tolerance, and ATR2 plays a supplementary role in Al tolerance in rice.",
keywords = "aluminum (Al) tolerance, aluminum resistance transcription factor 2 (ART2), regulation, rice, transcription factor",
author = "Jing Che and Tomokazu Tsutsui and Kengo Yokosho and Naoki Yamaji and Ma, {Jian Feng}",
year = "2018",
month = "10",
day = "1",
doi = "10.1111/nph.15252",
language = "English",
volume = "220",
pages = "209--218",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Functional characterization of an aluminum (Al)-inducible transcription factor, ART2, revealed a different pathway for Al tolerance in rice

AU - Che, Jing

AU - Tsutsui, Tomokazu

AU - Yokosho, Kengo

AU - Yamaji, Naoki

AU - Ma, Jian Feng

PY - 2018/10/1

Y1 - 2018/10/1

N2 - High aluminum (Al) tolerance in rice (Oryza sativa) is controlled by a Cys2His2-type zinc finger transcription factor ART1 (Al resistance transcription factor 1). There are five close homologs of ART1 in the rice genome, but the role of these homologs is unknown. We functionally characterized one of the ART1 homologs, ART2, in terms of tissue and spatial expression, subcellular localization, transcriptional activation activity, and phenotypic analysis of the knockout lines. ART2 was localized to the nucleus and showed a transcriptional activation potential in yeast. ART2 was mainly expressed in the roots, but the expression level was much lower than that of ART1. The ART2 expression was rapidly induced by Al in the roots of the wild-type rice, but not in art1 mutant. Knockout of ART2 resulted in increased sensitivity to Al toxicity, but did not alter sensitivity to different pH values. Expression profile analysis by RNA-sequencing showed that ART2 was not involved in activation of genes regulated by ART1; however, four genes seems to be regulated by ART2, which are implicated in Al tolerance. These results indicate that ART1 and ART2 regulate different pathways leading to Al tolerance, and ATR2 plays a supplementary role in Al tolerance in rice.

AB - High aluminum (Al) tolerance in rice (Oryza sativa) is controlled by a Cys2His2-type zinc finger transcription factor ART1 (Al resistance transcription factor 1). There are five close homologs of ART1 in the rice genome, but the role of these homologs is unknown. We functionally characterized one of the ART1 homologs, ART2, in terms of tissue and spatial expression, subcellular localization, transcriptional activation activity, and phenotypic analysis of the knockout lines. ART2 was localized to the nucleus and showed a transcriptional activation potential in yeast. ART2 was mainly expressed in the roots, but the expression level was much lower than that of ART1. The ART2 expression was rapidly induced by Al in the roots of the wild-type rice, but not in art1 mutant. Knockout of ART2 resulted in increased sensitivity to Al toxicity, but did not alter sensitivity to different pH values. Expression profile analysis by RNA-sequencing showed that ART2 was not involved in activation of genes regulated by ART1; however, four genes seems to be regulated by ART2, which are implicated in Al tolerance. These results indicate that ART1 and ART2 regulate different pathways leading to Al tolerance, and ATR2 plays a supplementary role in Al tolerance in rice.

KW - aluminum (Al) tolerance

KW - aluminum resistance transcription factor 2 (ART2)

KW - regulation

KW - rice

KW - transcription factor

UR - http://www.scopus.com/inward/record.url?scp=85052510325&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85052510325&partnerID=8YFLogxK

U2 - 10.1111/nph.15252

DO - 10.1111/nph.15252

M3 - Article

C2 - 29888411

AN - SCOPUS:85052510325

VL - 220

SP - 209

EP - 218

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 1

ER -