Functional characterization of a novel plasma membrane intrinsic protein2 in barley

Mineo Shibasaka, Sizuka Sasano, Sigeko Utsugi, Maki Katsuhara

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Water homeostasis is crucial to the growth and survival of plants. Plasma membrane intrinsic proteins (PIPs) have been shown to be primary channels mediating water uptake in plant cells. We characterized a novel PIP2 gene, HvPIP2;8 in barley (Hordeum vulgare). HvPIP2;8 shared 72-76% identity with other HvPIP2s and 74% identity with rice OsPIP2;8. The gene was expressed in all organs including the shoots, roots and pistil at a similar level. When HvPIP2;8 was transiently expressed in onion epidermal cells, it was localized to the plasma membrane. HvPIP2;8 showed transport activity for water in Xenopus oocytes, however its interaction with HvPIP1;2 was not observed. These results suggest that HvPIP2;8 plays a role in water homeostasis although further functional analysis is required in future.

Original languageEnglish
JournalPlant Signaling and Behavior
Volume7
Issue number12
DOIs
Publication statusPublished - Dec 21 2012

Keywords

  • Aquaporin
  • Barley
  • Hordeum vulgare
  • HvPIP2;8
  • PIP
  • Plasma membrane intrinsic protein
  • Water transport
  • Xenopus laevis oocytes

ASJC Scopus subject areas

  • Plant Science

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