Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon

Ahmet L. Tek, Kazunari Kashihara, Minoru Murata, Kiyotaka Nagaki

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present.

Original languageEnglish
Pages (from-to)337-347
Number of pages11
JournalChromosome Research
Volume18
Issue number3
DOIs
Publication statusPublished - Apr 2010

Fingerprint

Retroelements
Tandem Repeat Sequences
Centromere
Soybeans
Kinetochores
Antibodies
Chromatin Immunoprecipitation
Cell Division
Histones
Genome
Genes
Proteins

Keywords

  • Centromere-associated retrotransposon of glycine max (GmCR)
  • Centromere-specific histoneh3
  • Kinetochore
  • Satellite repeat
  • Soybean

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon. / Tek, Ahmet L.; Kashihara, Kazunari; Murata, Minoru; Nagaki, Kiyotaka.

In: Chromosome Research, Vol. 18, No. 3, 04.2010, p. 337-347.

Research output: Contribution to journalArticle

Tek, Ahmet L. ; Kashihara, Kazunari ; Murata, Minoru ; Nagaki, Kiyotaka. / Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon. In: Chromosome Research. 2010 ; Vol. 18, No. 3. pp. 337-347.
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