Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon

Ahmet L. Tek; Kazunari Kashihara; Minoru Murata; Kiyotaka Nagaki

doi:10.1007/s10577-010-9119-x

Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon

Ahmet L. Tek, Kazunari Kashihara, Minoru Murata, Kiyotaka Nagaki

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42 Citations (Scopus)

Abstract

The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present.

Original language	English
Pages (from-to)	337-347
Number of pages	11
Journal	Chromosome Research
Volume	18
Issue number	3
DOIs	https://doi.org/10.1007/s10577-010-9119-x
Publication status	Published - Apr 2010

Keywords

Centromere-associated retrotransposon of glycine max (GmCR)
Centromere-specific histoneh3
Kinetochore
Satellite repeat
Soybean

ASJC Scopus subject areas

Genetics

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10.1007/s10577-010-9119-x

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title = "Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon",

abstract = "The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present.",

keywords = "Centromere-associated retrotransposon of glycine max (GmCR), Centromere-specific histoneh3, Kinetochore, Satellite repeat, Soybean",

author = "Tek, {Ahmet L.} and Kazunari Kashihara and Minoru Murata and Kiyotaka Nagaki",

note = "Funding Information: Acknowledgements We thank Dr. Jiri Macas for the help in sequence analysis. This work was supported by the Fellowship Program of the Japan Society for the Promotion of Science (JSPS) to ALT and KN.",

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doi = "10.1007/s10577-010-9119-x",

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AU - Tek, Ahmet L.

AU - Kashihara, Kazunari

AU - Murata, Minoru

AU - Nagaki, Kiyotaka

N1 - Funding Information: Acknowledgements We thank Dr. Jiri Macas for the help in sequence analysis. This work was supported by the Fellowship Program of the Japan Society for the Promotion of Science (JSPS) to ALT and KN.

PY - 2010/4

Y1 - 2010/4

N2 - The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present.

AB - The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present.

KW - Centromere-associated retrotransposon of glycine max (GmCR)

KW - Centromere-specific histoneh3

KW - Kinetochore

KW - Satellite repeat

KW - Soybean

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Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon

Abstract

Keywords

ASJC Scopus subject areas

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