Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon

Ahmet L. Tek; Kazunari Kashihara; Minoru Murata; Kiyotaka Nagaki

doi:10.1007/s10577-010-9119-x

Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon

Ahmet L. Tek, Kazunari Kashihara, Minoru Murata, Kiyotaka Nagaki

Institute of Plant Science and Resources

Research output: Contribution to journal › Article

34 Citations (Scopus)

Abstract

The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present.

Original language	English
Pages (from-to)	337-347
Number of pages	11
Journal	Chromosome Research
Volume	18
Issue number	3
DOIs	https://doi.org/10.1007/s10577-010-9119-x
Publication status	Published - Apr 1 2010

Keywords

Centromere-associated retrotransposon of glycine max (GmCR)
Centromere-specific histoneh3
Kinetochore
Satellite repeat
Soybean

ASJC Scopus subject areas

Genetics

Access to Document

10.1007/s10577-010-9119-x

Fingerprint Dive into the research topics of 'Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon'. Together they form a unique fingerprint.

Cite this

Tek, A. L., Kashihara, K., Murata, M., & Nagaki, K. (2010). Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon. Chromosome Research, 18(3), 337-347. https://doi.org/10.1007/s10577-010-9119-x

@article{926bdba541ae4beb85588e55dca3a0dd,

title = "Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon",

abstract = "The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present.",

keywords = "Centromere-associated retrotransposon of glycine max (GmCR), Centromere-specific histoneh3, Kinetochore, Satellite repeat, Soybean",

author = "Tek, {Ahmet L.} and Kazunari Kashihara and Minoru Murata and Kiyotaka Nagaki",

year = "2010",

month = apr,

day = "1",

doi = "10.1007/s10577-010-9119-x",

language = "English",

volume = "18",

pages = "337--347",

journal = "Chromosome Research",

issn = "0967-3849",

publisher = "Springer Netherlands",

number = "3",

}

TY - JOUR

T1 - Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon

AU - Tek, Ahmet L.

AU - Kashihara, Kazunari

AU - Murata, Minoru

AU - Nagaki, Kiyotaka

PY - 2010/4/1

Y1 - 2010/4/1

N2 - The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present.

AB - The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present.

KW - Centromere-associated retrotransposon of glycine max (GmCR)

KW - Centromere-specific histoneh3

KW - Kinetochore

KW - Satellite repeat

KW - Soybean

UR - http://www.scopus.com/inward/record.url?scp=77954563246&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954563246&partnerID=8YFLogxK

U2 - 10.1007/s10577-010-9119-x

DO - 10.1007/s10577-010-9119-x

M3 - Article

C2 - 20204495

AN - SCOPUS:77954563246

VL - 18

SP - 337

EP - 347

JO - Chromosome Research

JF - Chromosome Research

SN - 0967-3849

IS - 3

ER -