Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon

Ahmet L. Tek; Kazunari Kashihara; Minoru Murata; Kiyotaka Nagaki

doi:10.1007/s10577-010-9119-x

Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon

Ahmet L. Tek, Kazunari Kashihara, Minoru Murata, Kiyotaka Nagaki

Institute of Plant Science and Resources

Research output: Contribution to journal › Article

36 Citations (Scopus)

Abstract

The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present.

Original language	English
Pages (from-to)	337-347
Number of pages	11
Journal	Chromosome Research
Volume	18
Issue number	3
DOIs	https://doi.org/10.1007/s10577-010-9119-x
Publication status	Published - Apr 1 2010

Keywords

Centromere-associated retrotransposon of glycine max (GmCR)
Centromere-specific histoneh3
Kinetochore
Satellite repeat
Soybean

ASJC Scopus subject areas

Genetics

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AU - Tek, Ahmet L.

AU - Kashihara, Kazunari

AU - Murata, Minoru

AU - Nagaki, Kiyotaka

PY - 2010/4/1

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