Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon

Ahmet L. Tek, Kazunari Kashihara, Minoru Murata, Kiyotaka Nagaki

    Research output: Contribution to journalArticlepeer-review

    42 Citations (Scopus)

    Abstract

    The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present.

    Original languageEnglish
    Pages (from-to)337-347
    Number of pages11
    JournalChromosome Research
    Volume18
    Issue number3
    DOIs
    Publication statusPublished - Apr 2010

    Keywords

    • Centromere-associated retrotransposon of glycine max (GmCR)
    • Centromere-specific histoneh3
    • Kinetochore
    • Satellite repeat
    • Soybean

    ASJC Scopus subject areas

    • Genetics

    Fingerprint

    Dive into the research topics of 'Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon'. Together they form a unique fingerprint.

    Cite this