Functional and molecular characteristics of rice and barley NIP aquaporins transporting water, hydrogen peroxide and arsenite

Maki Katsuhara, Shizuka Sasano, Tomoaki Horie, Tadashi Matsumoto, Jiye Rhee, Mineo Shibasaka

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Mercury-sensitive water transport activities were detected in seven NIP (Nodulin 26-like intrinsic protein) type aquaporins among eleven NIPs examined. Amino acid substitutions in rice OsNIP3;3 revealed that mercury-sensitivity depended on a histidine (but not on a cysteine) in apoplastic loop C in plant NIP aquaporins, although the cysteine is involved in the mercury-sensitivity of animal aquaporins. Rice OsNIP3;3 was also first identified as a unique aquaporin facilitating all water, hydrogen peroxide and arsenite transports. In rice OsNIP3;2, hydrogen peroxide and arsenite transport activities were detected, but water transport was not. Barley HvNIP1;2- or rice OsNIP2;1-expressing yeast cells showed the arsenite transport activity but not the H2O2transport activity. The present work revealed novel molecular mechanisms of water and other low molecular weight compounds transport/selection in barley and rice NIP aquaporins, including the histidine-related mercury-sensitivity in the water transport of aquaporins.

Original languageEnglish
Pages (from-to)213-219
Number of pages7
JournalPlant Biotechnology
Volume31
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

nodulins
arsenites
Aquaporins
aquaporins
Hordeum
Hydrogen Peroxide
hydrogen peroxide
barley
Mercury
rice
mercury
Water
Proteins
proteins
water
histidine
Histidine
Cysteine
cysteine
amino acid substitution

Keywords

  • Aromatic/arginine filter
  • Arsenite
  • Hydrogen peroxide
  • Mercury-sensitive water transport
  • NIP aquaporins

ASJC Scopus subject areas

  • Plant Science
  • Biotechnology
  • Agronomy and Crop Science

Cite this

Functional and molecular characteristics of rice and barley NIP aquaporins transporting water, hydrogen peroxide and arsenite. / Katsuhara, Maki; Sasano, Shizuka; Horie, Tomoaki; Matsumoto, Tadashi; Rhee, Jiye; Shibasaka, Mineo.

In: Plant Biotechnology, Vol. 31, No. 3, 2014, p. 213-219.

Research output: Contribution to journalArticle

Katsuhara, Maki ; Sasano, Shizuka ; Horie, Tomoaki ; Matsumoto, Tadashi ; Rhee, Jiye ; Shibasaka, Mineo. / Functional and molecular characteristics of rice and barley NIP aquaporins transporting water, hydrogen peroxide and arsenite. In: Plant Biotechnology. 2014 ; Vol. 31, No. 3. pp. 213-219.
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