Abstract
Mercury-sensitive water transport activities were detected in seven NIP (Nodulin 26-like intrinsic protein) type aquaporins among eleven NIPs examined. Amino acid substitutions in rice OsNIP3;3 revealed that mercury-sensitivity depended on a histidine (but not on a cysteine) in apoplastic loop C in plant NIP aquaporins, although the cysteine is involved in the mercury-sensitivity of animal aquaporins. Rice OsNIP3;3 was also first identified as a unique aquaporin facilitating all water, hydrogen peroxide and arsenite transports. In rice OsNIP3;2, hydrogen peroxide and arsenite transport activities were detected, but water transport was not. Barley HvNIP1;2- or rice OsNIP2;1-expressing yeast cells showed the arsenite transport activity but not the H2O2transport activity. The present work revealed novel molecular mechanisms of water and other low molecular weight compounds transport/selection in barley and rice NIP aquaporins, including the histidine-related mercury-sensitivity in the water transport of aquaporins.
| Original language | English |
|---|---|
| Pages (from-to) | 213-219 |
| Number of pages | 7 |
| Journal | Plant Biotechnology |
| Volume | 31 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Aromatic/arginine filter
- Arsenite
- Hydrogen peroxide
- Mercury-sensitive water transport
- NIP aquaporins
ASJC Scopus subject areas
- Plant Science
- Biotechnology
- Agronomy and Crop Science
Cite this
Functional and molecular characteristics of rice and barley NIP aquaporins transporting water, hydrogen peroxide and arsenite. / Katsuhara, Maki; Sasano, Shizuka; Horie, Tomoaki; Matsumoto, Tadashi; Rhee, Jiye; Shibasaka, Mineo.
In: Plant Biotechnology, Vol. 31, No. 3, 2014, p. 213-219.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional and molecular characteristics of rice and barley NIP aquaporins transporting water, hydrogen peroxide and arsenite
AU - Katsuhara, Maki
AU - Sasano, Shizuka
AU - Horie, Tomoaki
AU - Matsumoto, Tadashi
AU - Rhee, Jiye
AU - Shibasaka, Mineo
PY - 2014
Y1 - 2014
N2 - Mercury-sensitive water transport activities were detected in seven NIP (Nodulin 26-like intrinsic protein) type aquaporins among eleven NIPs examined. Amino acid substitutions in rice OsNIP3;3 revealed that mercury-sensitivity depended on a histidine (but not on a cysteine) in apoplastic loop C in plant NIP aquaporins, although the cysteine is involved in the mercury-sensitivity of animal aquaporins. Rice OsNIP3;3 was also first identified as a unique aquaporin facilitating all water, hydrogen peroxide and arsenite transports. In rice OsNIP3;2, hydrogen peroxide and arsenite transport activities were detected, but water transport was not. Barley HvNIP1;2- or rice OsNIP2;1-expressing yeast cells showed the arsenite transport activity but not the H2O2transport activity. The present work revealed novel molecular mechanisms of water and other low molecular weight compounds transport/selection in barley and rice NIP aquaporins, including the histidine-related mercury-sensitivity in the water transport of aquaporins.
AB - Mercury-sensitive water transport activities were detected in seven NIP (Nodulin 26-like intrinsic protein) type aquaporins among eleven NIPs examined. Amino acid substitutions in rice OsNIP3;3 revealed that mercury-sensitivity depended on a histidine (but not on a cysteine) in apoplastic loop C in plant NIP aquaporins, although the cysteine is involved in the mercury-sensitivity of animal aquaporins. Rice OsNIP3;3 was also first identified as a unique aquaporin facilitating all water, hydrogen peroxide and arsenite transports. In rice OsNIP3;2, hydrogen peroxide and arsenite transport activities were detected, but water transport was not. Barley HvNIP1;2- or rice OsNIP2;1-expressing yeast cells showed the arsenite transport activity but not the H2O2transport activity. The present work revealed novel molecular mechanisms of water and other low molecular weight compounds transport/selection in barley and rice NIP aquaporins, including the histidine-related mercury-sensitivity in the water transport of aquaporins.
KW - Aromatic/arginine filter
KW - Arsenite
KW - Hydrogen peroxide
KW - Mercury-sensitive water transport
KW - NIP aquaporins
UR - http://www.scopus.com/inward/record.url?scp=84929858299&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84929858299&partnerID=8YFLogxK
U2 - 10.5511/plantbiotechnology.14.0421a
DO - 10.5511/plantbiotechnology.14.0421a
M3 - Article
AN - SCOPUS:84929858299
VL - 31
SP - 213
EP - 219
JO - Plant Biotechnology
JF - Plant Biotechnology
SN - 1342-4580
IS - 3
ER -