Functional analysis of water channels in barley roots

Maki Katsuhara, Yoshiko Akiyama, Kazuki Koshio, Mineo Shibasaka, Kunihiro Kasamo

Research output: Contribution to journalArticlepeer-review

106 Citations (Scopus)

Abstract

We identified three genes homologous to water channels in the plasma membrane type subfamily from roots of barley seedlings. These genes were designated HvPIP2;1, HvPIP1;3, and HvPIP1;5 after comparison to Arabidopsis aquaporins. Competitive reverse transcription (RT)-PCR was applied in order to distinguish and to quantify their transcripts. The HvPIP2;1 transcript was the most abundant among the three in roots. Salt stress (200 mM NaCl) down-regulated HvPIP2;1 (transcript and protein), but had almost no effect on the expressions of HvPIP1;3, or HvPIP1;5. Approximately equal amounts of the transcripts of the three were detected in shoots, and salt stress enhanced the expression of HvPIP2;1 but not of HvPIP1;3, or HvP1P1;5. HvPIP2;1 protein was confirmed to be localized in the plasma membrane. Functional expression of HvPIP2;1 in Xenopus oocytes confirmed that HvPIP2;1 encoded an aquaporin that transports water. This water permeability was reduced by HgCl2, which is a typical water channel inhibitor. This activity was not modified by some inhibitors against protein kinase and protein phosphatase.

Original languageEnglish
Pages (from-to)885-893
Number of pages9
JournalPlant and Cell Physiology
Volume43
Issue number8
DOIs
Publication statusPublished - Aug 2002

Keywords

  • Aquaporin
  • Barley
  • Competitive RT-PCR
  • Salt stress

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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