Functional analysis of water channels in barley roots

Maki Katsuhara, Yoshiko Akiyama, Kazuki Koshio, Mineo Shibasaka, Kunihiro Kasamo

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

We identified three genes homologous to water channels in the plasma membrane type subfamily from roots of barley seedlings. These genes were designated HvPIP2;1, HvPIP1;3, and HvPIP1;5 after comparison to Arabidopsis aquaporins. Competitive reverse transcription (RT)-PCR was applied in order to distinguish and to quantify their transcripts. The HvPIP2;1 transcript was the most abundant among the three in roots. Salt stress (200 mM NaCl) down-regulated HvPIP2;1 (transcript and protein), but had almost no effect on the expressions of HvPIP1;3, or HvPIP1;5. Approximately equal amounts of the transcripts of the three were detected in shoots, and salt stress enhanced the expression of HvPIP2;1 but not of HvPIP1;3, or HvP1P1;5. HvPIP2;1 protein was confirmed to be localized in the plasma membrane. Functional expression of HvPIP2;1 in Xenopus oocytes confirmed that HvPIP2;1 encoded an aquaporin that transports water. This water permeability was reduced by HgCl2, which is a typical water channel inhibitor. This activity was not modified by some inhibitors against protein kinase and protein phosphatase.

Original languageEnglish
Pages (from-to)885-893
Number of pages9
JournalPlant and Cell Physiology
Volume43
Issue number8
DOIs
Publication statusPublished - Aug 2002

Keywords

  • Aquaporin
  • Barley
  • Competitive RT-PCR
  • Salt stress

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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