Functional analysis of the role of eyes absent and sine oculis in the developing eye of the cricket Gryllus bimaculatus

Akira Takagi, Kazuki Kurita, Taiki Terasawa, Taro Nakamura, Tetsuya Bando, Yoshiyuki Moriyama, Taro Mito, Sumihare Noji, Hideyo Ohuchi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In the cricket Gryllus bimaculatus, a hemimetabolous insect, the compound eyes begin to form in the embryo and increase 5-6 fold in size during the postembryonic development of the nymphal stage. Retinal stem cells in the anteroventral proliferation zone (AVPZ) of the nymphal eye proliferate to increase retinal progenitors, which then differentiate to form new ommatidia in the anterior region of the eye. However, mechanisms underlying this type of eye formation have not been well elucidated yet. Here, we found that the homologues of the retinal determination transcription factor genes of eyes absent (eya) and sine oculis (so) are expressed during the cricket embryonic eye formation. eya is also expressed intensely in the AVPZ of the nymphal eye. To explore their functions, we performed knockdown by RNA interference (RNAi). Knockdown of Gb'eya resulted in loss of the embryonic eye. In the nymphal eye, RNAi against Gb'eya or Gb'so impaired retinal morphology by apparently transforming cornea structures into head cuticle. These results imply that Gb'eya and Gb'so are essential for the differentiation of the retinal progenitor cells and maintaining retinal structures during eye development.

Original languageEnglish
Pages (from-to)227-240
Number of pages14
JournalDevelopment Growth and Differentiation
Volume54
Issue number2
DOIs
Publication statusPublished - Feb 2012
Externally publishedYes

Fingerprint

Gryllidae
RNA Interference
tetrahydrozoline
Stem Cells
Cornea
Insects

Keywords

  • Anteroventral proliferation zone
  • Eye development
  • Eyes absent
  • Gryllus bimaculatus
  • RNA interference
  • Sine oculis

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Functional analysis of the role of eyes absent and sine oculis in the developing eye of the cricket Gryllus bimaculatus. / Takagi, Akira; Kurita, Kazuki; Terasawa, Taiki; Nakamura, Taro; Bando, Tetsuya; Moriyama, Yoshiyuki; Mito, Taro; Noji, Sumihare; Ohuchi, Hideyo.

In: Development Growth and Differentiation, Vol. 54, No. 2, 02.2012, p. 227-240.

Research output: Contribution to journalArticle

Takagi, Akira ; Kurita, Kazuki ; Terasawa, Taiki ; Nakamura, Taro ; Bando, Tetsuya ; Moriyama, Yoshiyuki ; Mito, Taro ; Noji, Sumihare ; Ohuchi, Hideyo. / Functional analysis of the role of eyes absent and sine oculis in the developing eye of the cricket Gryllus bimaculatus. In: Development Growth and Differentiation. 2012 ; Vol. 54, No. 2. pp. 227-240.
@article{bc6d5403c03a43acaa375b28e71c818e,
title = "Functional analysis of the role of eyes absent and sine oculis in the developing eye of the cricket Gryllus bimaculatus",
abstract = "In the cricket Gryllus bimaculatus, a hemimetabolous insect, the compound eyes begin to form in the embryo and increase 5-6 fold in size during the postembryonic development of the nymphal stage. Retinal stem cells in the anteroventral proliferation zone (AVPZ) of the nymphal eye proliferate to increase retinal progenitors, which then differentiate to form new ommatidia in the anterior region of the eye. However, mechanisms underlying this type of eye formation have not been well elucidated yet. Here, we found that the homologues of the retinal determination transcription factor genes of eyes absent (eya) and sine oculis (so) are expressed during the cricket embryonic eye formation. eya is also expressed intensely in the AVPZ of the nymphal eye. To explore their functions, we performed knockdown by RNA interference (RNAi). Knockdown of Gb'eya resulted in loss of the embryonic eye. In the nymphal eye, RNAi against Gb'eya or Gb'so impaired retinal morphology by apparently transforming cornea structures into head cuticle. These results imply that Gb'eya and Gb'so are essential for the differentiation of the retinal progenitor cells and maintaining retinal structures during eye development.",
keywords = "Anteroventral proliferation zone, Eye development, Eyes absent, Gryllus bimaculatus, RNA interference, Sine oculis",
author = "Akira Takagi and Kazuki Kurita and Taiki Terasawa and Taro Nakamura and Tetsuya Bando and Yoshiyuki Moriyama and Taro Mito and Sumihare Noji and Hideyo Ohuchi",
year = "2012",
month = "2",
doi = "10.1111/j.1440-169X.2011.01325.x",
language = "English",
volume = "54",
pages = "227--240",
journal = "Development Growth and Differentiation",
issn = "0012-1592",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Functional analysis of the role of eyes absent and sine oculis in the developing eye of the cricket Gryllus bimaculatus

AU - Takagi, Akira

AU - Kurita, Kazuki

AU - Terasawa, Taiki

AU - Nakamura, Taro

AU - Bando, Tetsuya

AU - Moriyama, Yoshiyuki

AU - Mito, Taro

AU - Noji, Sumihare

AU - Ohuchi, Hideyo

PY - 2012/2

Y1 - 2012/2

N2 - In the cricket Gryllus bimaculatus, a hemimetabolous insect, the compound eyes begin to form in the embryo and increase 5-6 fold in size during the postembryonic development of the nymphal stage. Retinal stem cells in the anteroventral proliferation zone (AVPZ) of the nymphal eye proliferate to increase retinal progenitors, which then differentiate to form new ommatidia in the anterior region of the eye. However, mechanisms underlying this type of eye formation have not been well elucidated yet. Here, we found that the homologues of the retinal determination transcription factor genes of eyes absent (eya) and sine oculis (so) are expressed during the cricket embryonic eye formation. eya is also expressed intensely in the AVPZ of the nymphal eye. To explore their functions, we performed knockdown by RNA interference (RNAi). Knockdown of Gb'eya resulted in loss of the embryonic eye. In the nymphal eye, RNAi against Gb'eya or Gb'so impaired retinal morphology by apparently transforming cornea structures into head cuticle. These results imply that Gb'eya and Gb'so are essential for the differentiation of the retinal progenitor cells and maintaining retinal structures during eye development.

AB - In the cricket Gryllus bimaculatus, a hemimetabolous insect, the compound eyes begin to form in the embryo and increase 5-6 fold in size during the postembryonic development of the nymphal stage. Retinal stem cells in the anteroventral proliferation zone (AVPZ) of the nymphal eye proliferate to increase retinal progenitors, which then differentiate to form new ommatidia in the anterior region of the eye. However, mechanisms underlying this type of eye formation have not been well elucidated yet. Here, we found that the homologues of the retinal determination transcription factor genes of eyes absent (eya) and sine oculis (so) are expressed during the cricket embryonic eye formation. eya is also expressed intensely in the AVPZ of the nymphal eye. To explore their functions, we performed knockdown by RNA interference (RNAi). Knockdown of Gb'eya resulted in loss of the embryonic eye. In the nymphal eye, RNAi against Gb'eya or Gb'so impaired retinal morphology by apparently transforming cornea structures into head cuticle. These results imply that Gb'eya and Gb'so are essential for the differentiation of the retinal progenitor cells and maintaining retinal structures during eye development.

KW - Anteroventral proliferation zone

KW - Eye development

KW - Eyes absent

KW - Gryllus bimaculatus

KW - RNA interference

KW - Sine oculis

UR - http://www.scopus.com/inward/record.url?scp=84862781162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862781162&partnerID=8YFLogxK

U2 - 10.1111/j.1440-169X.2011.01325.x

DO - 10.1111/j.1440-169X.2011.01325.x

M3 - Article

C2 - 22348272

AN - SCOPUS:84862781162

VL - 54

SP - 227

EP - 240

JO - Development Growth and Differentiation

JF - Development Growth and Differentiation

SN - 0012-1592

IS - 2

ER -