Functional analysis of the flagellar genes in the fliD operon of Salmonella typhimurium

T. Yokoseki, K. Kutsukake, K. Ohnishi, T. Iino

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The fliD genes of Salmonella typhimurium and Escherichia coli encode the filament-cap protein of the flagellar apparatus, which facilitates the polymerization of endogenous flagellin at the tips of the growing filaments. Previous sequence analysis of this operon in both organisms has revealed that the fliD gene constitutes an operon together with two additional genes, fliS and fliT. Based on the gene-disruption experiment in E. coli, both the fliS and fliT genes have been postulated to be necessary for flagellation. In the present study, we constructed S. typhimurium mutants in which either fliS or fliT on the chromosome was specifically disrupted. Both mutants were found to produce functional flagella, indicating that these genes are dispensable for motility devolvement in S. typhimurium. However, flagellar filaments produced by the fliS mutant were much shorter than those produced by the wild-type strain. This indicates that the fliS mutation affects the elongation step of filament assembly. The excretion efficiency of flagellin was examined in the fliD-mutant background, where the exported flagellin molecules cannot assemble onto the hooks, resulting in their excretion into the culture media. We found that the amount of flagellin excreted was much reduced by the fliS mutation. Based on these results, we conclude that FliS facilitates the export of flagellin through the flagellum-specific export pathway.

Original languageEnglish
Pages (from-to)1715-1722
Number of pages8
JournalMicrobiology
Volume141
Issue number7
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Flagellin
Salmonella typhimurium
Operon
Genes
Flagella
Escherichia coli
Mutation
Polymerization
Sequence Analysis
Culture Media
Chromosomes
Proteins

Keywords

  • Export pathway
  • Filament elongation
  • Flagellar gene
  • Flagellum-specific
  • Gene disruption
  • Salmonella

ASJC Scopus subject areas

  • Microbiology

Cite this

Yokoseki, T., Kutsukake, K., Ohnishi, K., & Iino, T. (1995). Functional analysis of the flagellar genes in the fliD operon of Salmonella typhimurium. Microbiology, 141(7), 1715-1722.

Functional analysis of the flagellar genes in the fliD operon of Salmonella typhimurium. / Yokoseki, T.; Kutsukake, K.; Ohnishi, K.; Iino, T.

In: Microbiology, Vol. 141, No. 7, 1995, p. 1715-1722.

Research output: Contribution to journalArticle

Yokoseki, T, Kutsukake, K, Ohnishi, K & Iino, T 1995, 'Functional analysis of the flagellar genes in the fliD operon of Salmonella typhimurium', Microbiology, vol. 141, no. 7, pp. 1715-1722.
Yokoseki T, Kutsukake K, Ohnishi K, Iino T. Functional analysis of the flagellar genes in the fliD operon of Salmonella typhimurium. Microbiology. 1995;141(7):1715-1722.
Yokoseki, T. ; Kutsukake, K. ; Ohnishi, K. ; Iino, T. / Functional analysis of the flagellar genes in the fliD operon of Salmonella typhimurium. In: Microbiology. 1995 ; Vol. 141, No. 7. pp. 1715-1722.
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