Functional analysis of the circadian clock gene period by RNA interference in nymphal crickets Gryllus bimaculatus

Yoshiyuki Moriyama, Tomoaki Sakamoto, Akira Matsumoto, Sumihare Noji, Kenji Tomioka

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The circadian clock gene period (Gryllus bimaculatus period, Gb'per) plays a core role in circadian rhythm generation in adults of the cricket Gryllus bimaculatus. We examined the role of Gb'per in nymphal crickets that show a diurnal rhythm rather than the nocturnal rhythm of the adults. As in the adult optic lobes, Gb'per mRNA levels in the head of the third instar nymphs showed daily cycling in light-dark cycles with a peak at mid night, and the rhythm persisted in constant darkness. Injection of Gb'per double-stranded RNA (dsRNA) into the abdomen of third instar nymphs knocked-down the mRNA levels to 25% of that in control animals. Most Gb'per dsRNA injected nymphs lost their circadian locomotor activity rhythm, while those injected with DsRed2 dsRNA as a negative control clearly maintained the rhythm. These results suggest that nymphs and adults share a common endogenous clock mechanism involving the clock gene Gb'per.

Original languageEnglish
Pages (from-to)396-400
Number of pages5
JournalJournal of Insect Physiology
Volume55
Issue number5
DOIs
Publication statusPublished - May 2009

Fingerprint

Gryllidae
Gryllus bimaculatus
Nymph
Circadian Clocks
RNA Interference
RNA interference
circadian rhythm
Double-Stranded RNA
Circadian Rhythm
nymphs
double-stranded RNA
Genes
genes
Messenger RNA
Darkness
Photoperiod
Locomotion
Abdomen
instars
optic lobe

Keywords

  • Circadian rhythm
  • Clock gene
  • Cricket
  • dsRNA
  • Locomotor activity
  • period
  • RNA interference

ASJC Scopus subject areas

  • Insect Science
  • Physiology

Cite this

Functional analysis of the circadian clock gene period by RNA interference in nymphal crickets Gryllus bimaculatus. / Moriyama, Yoshiyuki; Sakamoto, Tomoaki; Matsumoto, Akira; Noji, Sumihare; Tomioka, Kenji.

In: Journal of Insect Physiology, Vol. 55, No. 5, 05.2009, p. 396-400.

Research output: Contribution to journalArticle

Moriyama, Yoshiyuki ; Sakamoto, Tomoaki ; Matsumoto, Akira ; Noji, Sumihare ; Tomioka, Kenji. / Functional analysis of the circadian clock gene period by RNA interference in nymphal crickets Gryllus bimaculatus. In: Journal of Insect Physiology. 2009 ; Vol. 55, No. 5. pp. 396-400.
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