Abstract
Primer extension of human liver poly(A)+ RNA revealed that the main transcription start site of the human alanine:glyoxylate aminotransferase gene (AGXT) is situated near 45 bp upstream from the translation start site. Deletion analysis using the 1203 bp 5′-flanking region of the AGXT gene and a luciferase reporter suggested that the promoter sequence is most likely located 2-325 bp upstream from the translation start site, possibly with enhancer elements 440-700 bp upstream. It was also suggested that the region -2 to -64 is important for the expression of the AGXT gene. The region -2 to -325 has two TATA boxes and some initiator elements.
| Original language | English |
|---|---|
| Pages (from-to) | 205-209 |
| Number of pages | 5 |
| Journal | BBA - Gene Structure and Expression |
| Volume | 1574 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Mar 19 2002 |
| Externally published | Yes |
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Keywords
- 5′-Flanking region
- AGXT gene
- Initiator element
- Promoter analysis
- TATA box
- Transcription initiation
ASJC Scopus subject areas
- Biochemistry
- Genetics
- Structural Biology
- Biophysics
Cite this
Functional analysis of the 5′-flanking region of the human alanine : glyoxylate aminotransferase gene AGXT. / Sato, Manabu; Toné, Shigenobu; Ishikawa, Tetsuya; Purdue, P. Edward; Danpure, Christopher J.; Minatogawa, Yohsuke.
In: BBA - Gene Structure and Expression, Vol. 1574, No. 2, 19.03.2002, p. 205-209.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional analysis of the 5′-flanking region of the human alanine
T2 - glyoxylate aminotransferase gene AGXT
AU - Sato, Manabu
AU - Toné, Shigenobu
AU - Ishikawa, Tetsuya
AU - Purdue, P. Edward
AU - Danpure, Christopher J.
AU - Minatogawa, Yohsuke
PY - 2002/3/19
Y1 - 2002/3/19
N2 - Primer extension of human liver poly(A)+ RNA revealed that the main transcription start site of the human alanine:glyoxylate aminotransferase gene (AGXT) is situated near 45 bp upstream from the translation start site. Deletion analysis using the 1203 bp 5′-flanking region of the AGXT gene and a luciferase reporter suggested that the promoter sequence is most likely located 2-325 bp upstream from the translation start site, possibly with enhancer elements 440-700 bp upstream. It was also suggested that the region -2 to -64 is important for the expression of the AGXT gene. The region -2 to -325 has two TATA boxes and some initiator elements.
AB - Primer extension of human liver poly(A)+ RNA revealed that the main transcription start site of the human alanine:glyoxylate aminotransferase gene (AGXT) is situated near 45 bp upstream from the translation start site. Deletion analysis using the 1203 bp 5′-flanking region of the AGXT gene and a luciferase reporter suggested that the promoter sequence is most likely located 2-325 bp upstream from the translation start site, possibly with enhancer elements 440-700 bp upstream. It was also suggested that the region -2 to -64 is important for the expression of the AGXT gene. The region -2 to -325 has two TATA boxes and some initiator elements.
KW - 5′-Flanking region
KW - AGXT gene
KW - Initiator element
KW - Promoter analysis
KW - TATA box
KW - Transcription initiation
UR - http://www.scopus.com/inward/record.url?scp=0037133721&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037133721&partnerID=8YFLogxK
U2 - 10.1016/S0167-4781(01)00285-8
DO - 10.1016/S0167-4781(01)00285-8
M3 - Article
C2 - 11955631
AN - SCOPUS:0037133721
VL - 1574
SP - 205
EP - 209
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 2
ER -