Functional analysis of the 5′-flanking region of the human alanine:glyoxylate aminotransferase gene AGXT

Manabu Sato; Shigenobu Toné; Tetsuya Ishikawa; P. Edward Purdue; Christopher J. Danpure; Yohsuke Minatogawa

doi:10.1016/S0167-4781(01)00285-8

Functional analysis of the 5′-flanking region of the human alanine:glyoxylate aminotransferase gene AGXT

Manabu Sato, Shigenobu Toné, Tetsuya Ishikawa, P. Edward Purdue, Christopher J. Danpure, Yohsuke Minatogawa

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Primer extension of human liver poly(A)⁺ RNA revealed that the main transcription start site of the human alanine:glyoxylate aminotransferase gene (AGXT) is situated near 45 bp upstream from the translation start site. Deletion analysis using the 1203 bp 5′-flanking region of the AGXT gene and a luciferase reporter suggested that the promoter sequence is most likely located 2-325 bp upstream from the translation start site, possibly with enhancer elements 440-700 bp upstream. It was also suggested that the region -2 to -64 is important for the expression of the AGXT gene. The region -2 to -325 has two TATA boxes and some initiator elements.

Original language	English
Pages (from-to)	205-209
Number of pages	5
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1574
Issue number	2
DOIs	https://doi.org/10.1016/S0167-4781(01)00285-8
Publication status	Published - Mar 19 2002
Externally published	Yes

Keywords

5′-Flanking region
AGXT gene
Initiator element
Promoter analysis
TATA box
Transcription initiation

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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Functional analysis of the 5′-flanking region of the human alanine:glyoxylate aminotransferase gene AGXT. / Sato, Manabu; Toné, Shigenobu; Ishikawa, Tetsuya; Purdue, P. Edward; Danpure, Christopher J.; Minatogawa, Yohsuke.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1574, No. 2, 19.03.2002, p. 205-209.

Research output: Contribution to journal › Article › peer-review

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title = "Functional analysis of the 5′-flanking region of the human alanine:glyoxylate aminotransferase gene AGXT",

abstract = "Primer extension of human liver poly(A)+ RNA revealed that the main transcription start site of the human alanine:glyoxylate aminotransferase gene (AGXT) is situated near 45 bp upstream from the translation start site. Deletion analysis using the 1203 bp 5′-flanking region of the AGXT gene and a luciferase reporter suggested that the promoter sequence is most likely located 2-325 bp upstream from the translation start site, possibly with enhancer elements 440-700 bp upstream. It was also suggested that the region -2 to -64 is important for the expression of the AGXT gene. The region -2 to -325 has two TATA boxes and some initiator elements.",

keywords = "5′-Flanking region, AGXT gene, Initiator element, Promoter analysis, TATA box, Transcription initiation",

author = "Manabu Sato and Shigenobu Ton{\'e} and Tetsuya Ishikawa and Purdue, {P. Edward} and Danpure, {Christopher J.} and Yohsuke Minatogawa",

note = "Funding Information: This work was supported in part by a Research Project Grant (Nos. 6-101, 7-106, 8-108, 9-112, 10-107, 11-105, and 12-106) from Kawasaki Medical School. ",

year = "2002",

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doi = "10.1016/S0167-4781(01)00285-8",

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AU - Sato, Manabu

AU - Toné, Shigenobu

AU - Ishikawa, Tetsuya

AU - Purdue, P. Edward

AU - Danpure, Christopher J.

AU - Minatogawa, Yohsuke

N1 - Funding Information: This work was supported in part by a Research Project Grant (Nos. 6-101, 7-106, 8-108, 9-112, 10-107, 11-105, and 12-106) from Kawasaki Medical School.

PY - 2002/3/19

Y1 - 2002/3/19

N2 - Primer extension of human liver poly(A)+ RNA revealed that the main transcription start site of the human alanine:glyoxylate aminotransferase gene (AGXT) is situated near 45 bp upstream from the translation start site. Deletion analysis using the 1203 bp 5′-flanking region of the AGXT gene and a luciferase reporter suggested that the promoter sequence is most likely located 2-325 bp upstream from the translation start site, possibly with enhancer elements 440-700 bp upstream. It was also suggested that the region -2 to -64 is important for the expression of the AGXT gene. The region -2 to -325 has two TATA boxes and some initiator elements.

AB - Primer extension of human liver poly(A)+ RNA revealed that the main transcription start site of the human alanine:glyoxylate aminotransferase gene (AGXT) is situated near 45 bp upstream from the translation start site. Deletion analysis using the 1203 bp 5′-flanking region of the AGXT gene and a luciferase reporter suggested that the promoter sequence is most likely located 2-325 bp upstream from the translation start site, possibly with enhancer elements 440-700 bp upstream. It was also suggested that the region -2 to -64 is important for the expression of the AGXT gene. The region -2 to -325 has two TATA boxes and some initiator elements.

KW - 5′-Flanking region

KW - AGXT gene

KW - Initiator element

KW - Promoter analysis

KW - TATA box

KW - Transcription initiation

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Functional analysis of the 5′-flanking region of the human alanine:glyoxylate aminotransferase gene AGXT

Abstract

Keywords

ASJC Scopus subject areas

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