Functional analysis of the 5′-flanking region of the human alanine:glyoxylate aminotransferase gene AGXT

Manabu Sato; Shigenobu Toné; Tetsuya Ishikawa; P. Edward Purdue; Christopher J. Danpure; Yohsuke Minatogawa

doi:10.1016/S0167-4781(01)00285-8

Functional analysis of the 5′-flanking region of the human alanine:glyoxylate aminotransferase gene AGXT

Manabu Sato, Shigenobu Toné, Tetsuya Ishikawa, P. Edward Purdue, Christopher J. Danpure, Yohsuke Minatogawa

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Primer extension of human liver poly(A)⁺ RNA revealed that the main transcription start site of the human alanine:glyoxylate aminotransferase gene (AGXT) is situated near 45 bp upstream from the translation start site. Deletion analysis using the 1203 bp 5′-flanking region of the AGXT gene and a luciferase reporter suggested that the promoter sequence is most likely located 2-325 bp upstream from the translation start site, possibly with enhancer elements 440-700 bp upstream. It was also suggested that the region -2 to -64 is important for the expression of the AGXT gene. The region -2 to -325 has two TATA boxes and some initiator elements.

Original language	English
Pages (from-to)	205-209
Number of pages	5
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1574
Issue number	2
DOIs	https://doi.org/10.1016/S0167-4781(01)00285-8
Publication status	Published - Mar 19 2002
Externally published	Yes

Keywords

5′-Flanking region
AGXT gene
Initiator element
Promoter analysis
TATA box
Transcription initiation

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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Functional analysis of the 5′-flanking region of the human alanine:glyoxylate aminotransferase gene AGXT. / Sato, Manabu; Toné, Shigenobu; Ishikawa, Tetsuya; Purdue, P. Edward; Danpure, Christopher J.; Minatogawa, Yohsuke.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1574, No. 2, 19.03.2002, p. 205-209.

Research output: Contribution to journal › Article › peer-review

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abstract = "Primer extension of human liver poly(A)+ RNA revealed that the main transcription start site of the human alanine:glyoxylate aminotransferase gene (AGXT) is situated near 45 bp upstream from the translation start site. Deletion analysis using the 1203 bp 5′-flanking region of the AGXT gene and a luciferase reporter suggested that the promoter sequence is most likely located 2-325 bp upstream from the translation start site, possibly with enhancer elements 440-700 bp upstream. It was also suggested that the region -2 to -64 is important for the expression of the AGXT gene. The region -2 to -325 has two TATA boxes and some initiator elements.",

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AU - Purdue, P. Edward

AU - Danpure, Christopher J.

AU - Minatogawa, Yohsuke

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