Abstract
Primer extension of human liver poly(A)+ RNA revealed that the main transcription start site of the human alanine:glyoxylate aminotransferase gene (AGXT) is situated near 45 bp upstream from the translation start site. Deletion analysis using the 1203 bp 5′-flanking region of the AGXT gene and a luciferase reporter suggested that the promoter sequence is most likely located 2-325 bp upstream from the translation start site, possibly with enhancer elements 440-700 bp upstream. It was also suggested that the region -2 to -64 is important for the expression of the AGXT gene. The region -2 to -325 has two TATA boxes and some initiator elements.
| Original language | English |
|---|---|
| Pages (from-to) | 205-209 |
| Number of pages | 5 |
| Journal | Biochimica et Biophysica Acta - Gene Structure and Expression |
| Volume | 1574 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Mar 19 2002 |
| Externally published | Yes |
Keywords
- 5′-Flanking region
- AGXT gene
- Initiator element
- Promoter analysis
- TATA box
- Transcription initiation
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics
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Cite this
Sato, M., Toné, S., Ishikawa, T., Purdue, P. E., Danpure, C. J., & Minatogawa, Y. (2002). Functional analysis of the 5′-flanking region of the human alanine:glyoxylate aminotransferase gene AGXT. Biochimica et Biophysica Acta - Gene Structure and Expression, 1574(2), 205-209. https://doi.org/10.1016/S0167-4781(01)00285-8