Functional analysis of the 5′-flanking region of the human alanine:glyoxylate aminotransferase gene AGXT

Manabu Sato, Shigenobu Toné, Tetsuya Ishikawa, P. Edward Purdue, Christopher J. Danpure, Yohsuke Minatogawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Primer extension of human liver poly(A)+ RNA revealed that the main transcription start site of the human alanine:glyoxylate aminotransferase gene (AGXT) is situated near 45 bp upstream from the translation start site. Deletion analysis using the 1203 bp 5′-flanking region of the AGXT gene and a luciferase reporter suggested that the promoter sequence is most likely located 2-325 bp upstream from the translation start site, possibly with enhancer elements 440-700 bp upstream. It was also suggested that the region -2 to -64 is important for the expression of the AGXT gene. The region -2 to -325 has two TATA boxes and some initiator elements.

Original languageEnglish
Pages (from-to)205-209
Number of pages5
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1574
Issue number2
DOIs
Publication statusPublished - Mar 19 2002
Externally publishedYes

Keywords

  • 5′-Flanking region
  • AGXT gene
  • Initiator element
  • Promoter analysis
  • TATA box
  • Transcription initiation

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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