Functional analysis of the 5′-flanking region of the human alanine: glyoxylate aminotransferase gene AGXT

Manabu Sato, Shigenobu Toné, Tetsuya Ishikawa, P. Edward Purdue, Christopher J. Danpure, Yohsuke Minatogawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Primer extension of human liver poly(A)+ RNA revealed that the main transcription start site of the human alanine:glyoxylate aminotransferase gene (AGXT) is situated near 45 bp upstream from the translation start site. Deletion analysis using the 1203 bp 5′-flanking region of the AGXT gene and a luciferase reporter suggested that the promoter sequence is most likely located 2-325 bp upstream from the translation start site, possibly with enhancer elements 440-700 bp upstream. It was also suggested that the region -2 to -64 is important for the expression of the AGXT gene. The region -2 to -325 has two TATA boxes and some initiator elements.

Original languageEnglish
Pages (from-to)205-209
Number of pages5
JournalBBA - Gene Structure and Expression
Volume1574
Issue number2
DOIs
Publication statusPublished - Mar 19 2002
Externally publishedYes

Fingerprint

Functional analysis
5' Flanking Region
Genes
TATA Box
Transcription Initiation Site
Luciferases
Liver
Alanine-glyoxylate transaminase
Messenger RNA

Keywords

  • 5′-Flanking region
  • AGXT gene
  • Initiator element
  • Promoter analysis
  • TATA box
  • Transcription initiation

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Functional analysis of the 5′-flanking region of the human alanine : glyoxylate aminotransferase gene AGXT. / Sato, Manabu; Toné, Shigenobu; Ishikawa, Tetsuya; Purdue, P. Edward; Danpure, Christopher J.; Minatogawa, Yohsuke.

In: BBA - Gene Structure and Expression, Vol. 1574, No. 2, 19.03.2002, p. 205-209.

Research output: Contribution to journalArticle

Sato, Manabu ; Toné, Shigenobu ; Ishikawa, Tetsuya ; Purdue, P. Edward ; Danpure, Christopher J. ; Minatogawa, Yohsuke. / Functional analysis of the 5′-flanking region of the human alanine : glyoxylate aminotransferase gene AGXT. In: BBA - Gene Structure and Expression. 2002 ; Vol. 1574, No. 2. pp. 205-209.
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