Functional analysis of retrotransposons in pea

Hidenori Kato, Permpong Sriprasertsak, Hikaru Seki, Yuki Ichinose, Tomonori Shiraishi, Tetsuji Yamada

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The 5'-upstream regions of the plant active defense genes in pea (PSPAL2 and PSCHS1) exhibit significant nucleotide sequence identity to part of a copia-type retrotransposon. To characterize the retrotransposon in pea putative reverse transcriptase sequences (Psr) were amplified by PCR from cDNA prepared from protoplasts derived from pea suspension cultured cells. Psr genes can be classified into at least eight subgroups (A~H) according to their nucleotide sequence similarities. A subgroup PsrC was induced by the treatment of etiolated pea epicotyls with fungal elicitor within 30 min but was hardly induced by protoplasting of pea suspension cultured cells, whereas subgroups of PsrA and PsrB were highly induced by protoplasting. Interestingly, the retrotransposon sequence present at the 5'-upstream region of PSCHS1 which was induced by the treatment with fungal elicitor belonged to subgroup PsrC. The retrotransposon might play an important role in reorganization of the genes and optimization of the gene expression in response to various environmental stimuli such as an attack by phytopathogens or protoplasting.

Original languageEnglish
Pages (from-to)933-941
Number of pages9
JournalPlant and Cell Physiology
Volume40
Issue number9
DOIs
Publication statusPublished - Jan 1 1999

Keywords

  • Chalcone synthase
  • Elicitor Phenylalanine ammonia-lyase
  • Protoplasting
  • Retrotransposon

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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    Kato, H., Sriprasertsak, P., Seki, H., Ichinose, Y., Shiraishi, T., & Yamada, T. (1999). Functional analysis of retrotransposons in pea. Plant and Cell Physiology, 40(9), 933-941. https://doi.org/10.1093/oxfordjournals.pcp.a029625