Functional analysis of psbV and a novel c-type cytochrome gene psbV2 of the thermophilic cyanobacterium Thermosynechococcus elongatus strain BP-1

Hiroshi Katoh, Suwako Itoh, Jian-Ren Shen, Masahiko Ikeuchi

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Cytochrome c-550 is an extrinsic protein associated with photosystem II (PSII) in cyanobacteria and lower eukaryotic algae and plays an important role in the water-splitting reaction. The gene (psbV) for cytochrome c-550 was cloned from the thermophilic cyanobacteria Thermosynechococcus (formerly Synechococcus) elongatus and T. (formerly Synechococcus) vulcanus. In both genomes, located downstream of psbV were a novel gene (designated psbV2) for a c-type cytochrome and petJ for cytochrome c-553. The deduced product of psbV2 showed composite similarities to psbV and petJ. Phenotype of psbV-disruptant in Thermosynechococcus was practically the same as that reported in Synechocystis sp. PCC 6803. Either psbV or psbV2 gene of T. elongatus was expressed in the psbV-disruptant of Synechocystis sp. PCC 6803, which resulted in recovery of the photoautotrophic growth. However, the enhanced requirement of Ca2+ or Cl- ions in the psbV-disruptant of Synechocystis was suppressed by expression of psbV but not by expression of psbV2. Thus, it is concluded that psbV2 can partly replace the role of psbV in PSII. The close tandem arrangement of psbV/psbV2/petJ implies that psbV2 was created by gene duplication and intergenic recombination during evolution.

Original languageEnglish
Pages (from-to)599-607
Number of pages9
JournalPlant and Cell Physiology
Volume42
Issue number6
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Thermosynechococcus elongatus
Cytochrome c Group
Thermosynechococcus
Synechocystis
cytochrome c
Cyanobacteria
cytochromes
Cytochromes c
Synechococcus
Synechocystis sp. PCC 6803
Photosystem II Protein Complex
algae
photosystem II
Synechococcus vulcanus
Synechococcus elongatus
Genes
Gene Duplication
genes
gene duplication
Genetic Recombination

Keywords

  • Cytochrome
  • Photosystem II
  • psbV
  • psbV2
  • Thermophilic cyanobacterium
  • Thermosynechococcus (Synechococcus) elongatus

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Functional analysis of psbV and a novel c-type cytochrome gene psbV2 of the thermophilic cyanobacterium Thermosynechococcus elongatus strain BP-1. / Katoh, Hiroshi; Itoh, Suwako; Shen, Jian-Ren; Ikeuchi, Masahiko.

In: Plant and Cell Physiology, Vol. 42, No. 6, 2001, p. 599-607.

Research output: Contribution to journalArticle

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abstract = "Cytochrome c-550 is an extrinsic protein associated with photosystem II (PSII) in cyanobacteria and lower eukaryotic algae and plays an important role in the water-splitting reaction. The gene (psbV) for cytochrome c-550 was cloned from the thermophilic cyanobacteria Thermosynechococcus (formerly Synechococcus) elongatus and T. (formerly Synechococcus) vulcanus. In both genomes, located downstream of psbV were a novel gene (designated psbV2) for a c-type cytochrome and petJ for cytochrome c-553. The deduced product of psbV2 showed composite similarities to psbV and petJ. Phenotype of psbV-disruptant in Thermosynechococcus was practically the same as that reported in Synechocystis sp. PCC 6803. Either psbV or psbV2 gene of T. elongatus was expressed in the psbV-disruptant of Synechocystis sp. PCC 6803, which resulted in recovery of the photoautotrophic growth. However, the enhanced requirement of Ca2+ or Cl- ions in the psbV-disruptant of Synechocystis was suppressed by expression of psbV but not by expression of psbV2. Thus, it is concluded that psbV2 can partly replace the role of psbV in PSII. The close tandem arrangement of psbV/psbV2/petJ implies that psbV2 was created by gene duplication and intergenic recombination during evolution.",
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