Functional analysis of alpha5beta1 integrin and lipid rafts in invasion of epithelial cells by Porphyromonas gingivalis using fluorescent beads coated with bacterial membrane vesicles.

Kayoko Tsuda, Nobumichi Furuta, Hiroaki Inaba, Shinji Kawai, Kentaro Hanada, Tamotsu Yoshimori, Atsuo Amano

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Porphyromonas gingivalis, a periodontal pathogen, was previously suggested to exploit alpha5beta1 integrin and lipid rafts to invade host cells. However, it is unknown if the functional roles of these host components are distinct from one another during bacterial invasion. In the present study, we analyzed the mechanisms underlying P. gingivalis invasion, using fluorescent beads coated with bacterial membrane vesicles (MV beads). Cholesterol depletion reagents including methyl-beta-cyclodextrin (MbetaCD) drastically inhibited the entry of MV beads into epithelial cells, while they were less effective on bead adhesion to the cells. Bead entry was also abolished in CHO cells deficient in sphingolipids, components of lipid rafts, whereas adhesion was negligibly influenced. Following MbetaCD treatment, downstream events leading to actin polymerization were abolished; however, alpha5beta1 integrin was recruited to beads attached to the cell surface. Dominant-negative Rho GTPase Rac1 abolished cellular engulfment of the beads, whereas dominant-negative Cdc42 did not. Following cellular interaction with the beads, Rac1 was found to be translocated to the lipid rafts fraction, which was inhibited by MbetaCD. These results suggest that alpha5beta1 integrin, independent of lipid rafts, promotes P. gingivalis adhesion to epithelial cells, while the subsequent uptake process requires lipid raft components for actin organization, with Rho GTPase Rac1.

Original languageEnglish
Pages (from-to)123-132
Number of pages10
JournalCell Structure and Function
Volume33
Issue number1
DOIs
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Integrin alpha5beta1
Porphyromonas gingivalis
Epithelial Cells
Lipids
Membranes
rho GTP-Binding Proteins
Actins
Sphingolipids
CHO Cells
Cell Adhesion
Polymerization
Cholesterol
methyl-beta-cyclodextrin

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Physiology

Cite this

Functional analysis of alpha5beta1 integrin and lipid rafts in invasion of epithelial cells by Porphyromonas gingivalis using fluorescent beads coated with bacterial membrane vesicles. / Tsuda, Kayoko; Furuta, Nobumichi; Inaba, Hiroaki; Kawai, Shinji; Hanada, Kentaro; Yoshimori, Tamotsu; Amano, Atsuo.

In: Cell Structure and Function, Vol. 33, No. 1, 2008, p. 123-132.

Research output: Contribution to journalArticle

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