Functional analysis of abscisic acid 8′-hydroxylase

Akira Endo, Mitsuhiro Kimura, Naoto Kawakami, Eiji Nambara

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Abscisic acid (ABA) plays an important role in the control of seed dormancy and germination. Identification of hormone metabolism genes from a particular plant species of interest is an essential step in hormone research. The function of these gene products is validated by biochemical analysis using heterologous expression systems, such as E. coli and yeast. ABA 8′-hydroxylase is a subfamily of P450 monooxygenases and is encoded by CYP707A genes. CYP707A catalyzes the committed step in the major ABA catabolic pathway. In this chapter, we describe the methods for RNA extraction from seeds, cloning the CYP707A cDNAs, protein expression in yeast, and biochemical analysis of their gene products.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages136-147
Number of pages12
Volume773
DOIs
Publication statusPublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume773
ISSN (Print)10643745

Fingerprint

Abscisic Acid
Genes
Yeasts
Plant Dormancy
Hormones
Germination
Mixed Function Oxygenases
Organism Cloning
Seeds
Complementary DNA
RNA
Escherichia coli
abscisic acid 8'-hydroxylase
Research
Proteins

Keywords

  • ABA 8′-hydroxylase
  • cDNA cloning
  • Heterologous expression
  • P450
  • RNA extraction
  • Seed

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Endo, A., Kimura, M., Kawakami, N., & Nambara, E. (2011). Functional analysis of abscisic acid 8′-hydroxylase. In Methods in Molecular Biology (Vol. 773, pp. 136-147). (Methods in Molecular Biology; Vol. 773). https://doi.org/10.1007/978-1-61779-231-1_9

Functional analysis of abscisic acid 8′-hydroxylase. / Endo, Akira; Kimura, Mitsuhiro; Kawakami, Naoto; Nambara, Eiji.

Methods in Molecular Biology. Vol. 773 2011. p. 136-147 (Methods in Molecular Biology; Vol. 773).

Research output: Chapter in Book/Report/Conference proceedingChapter

Endo, A, Kimura, M, Kawakami, N & Nambara, E 2011, Functional analysis of abscisic acid 8′-hydroxylase. in Methods in Molecular Biology. vol. 773, Methods in Molecular Biology, vol. 773, pp. 136-147. https://doi.org/10.1007/978-1-61779-231-1_9
Endo A, Kimura M, Kawakami N, Nambara E. Functional analysis of abscisic acid 8′-hydroxylase. In Methods in Molecular Biology. Vol. 773. 2011. p. 136-147. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-231-1_9
Endo, Akira ; Kimura, Mitsuhiro ; Kawakami, Naoto ; Nambara, Eiji. / Functional analysis of abscisic acid 8′-hydroxylase. Methods in Molecular Biology. Vol. 773 2011. pp. 136-147 (Methods in Molecular Biology).
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