Functional analysis of abscisic acid 8′-hydroxylase

Akira Endo, Mitsuhiro Kimura, Naoto Kawakami, Eiji Nambara

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Abscisic acid (ABA) plays an important role in the control of seed dormancy and germination. Identification of hormone metabolism genes from a particular plant species of interest is an essential step in hormone research. The function of these gene products is validated by biochemical analysis using heterologous expression systems, such as E. coli and yeast. ABA 8′-hydroxylase is a subfamily of P450 monooxygenases and is encoded by CYP707A genes. CYP707A catalyzes the committed step in the major ABA catabolic pathway. In this chapter, we describe the methods for RNA extraction from seeds, cloning the CYP707A cDNAs, protein expression in yeast, and biochemical analysis of their gene products.

Original languageEnglish
Title of host publicationSeed Dormancy
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages136-147
Number of pages12
ISBN (Print)9781617792304
DOIs
Publication statusPublished - Jan 1 2011

Publication series

NameMethods in Molecular Biology
Volume773
ISSN (Print)1064-3745

Keywords

  • ABA 8′-hydroxylase
  • Heterologous expression
  • P450
  • RNA extraction
  • Seed
  • cDNA cloning

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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