Function of the nuclear receptor FTZ-F1 during the pupal stage in Drosophila melanogaster

Abdel Rahman S Sultan, Yasuhiro Oish, Hitoshi Ueda

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The nuclear receptor βFTZ-F1 is expressed in most cells in a temporally specific manner, and its expression is induced immediately after decline in ecdysteroid levels. This factor plays important roles during embryogenesis, larval ecdysis, and early metamorphic stages. However, little is known about the expression pattern, regulation and function of this receptor during the pupal stage. We analyzed the expression pattern and regulation of ftz-f1 during the pupal period, as well as the phenotypes of RNAi knockdown or mutant animals, to elucidate its function during this stage. Western blotting revealed that βFTZ-F1 is expressed at a high level during the late pupal stage, and this expression is dependent on decreasing ecdysteroid levels. By immunohistological analysis of the late pupal stage, FTZ-F1 was detected in the nuclei of most cells, but cytoplasmic localization was observed only in the oogonia and follicle cells of the ovary. Both the ftz-f1 genetic mutant and temporally specific ftz-f1 knockdown using RNAi during the pupal stage showed defects in eclosion and in the eye, the antennal segment, the wing and the leg, including bristle color and sclerosis. These results suggest that βFTZ-F1 is expressed in most cells at the late pupal stage, under the control of ecdysteroids and plays important roles during pupal development.

Original languageEnglish
Pages (from-to)245-253
Number of pages9
JournalDevelopment Growth and Differentiation
Volume56
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

Ecdysteroids
Cytoplasmic and Nuclear Receptors
Drosophila melanogaster
RNA Interference
Oogonia
Molting
Sclerosis
Cell Nucleus
Embryonic Development
Ovary
Leg
Color
Western Blotting
Phenotype

Keywords

  • Ecdysteroid
  • Metamorphosis
  • Nuclear receptor
  • Pupal development
  • βFTZ-F1

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Medicine(all)

Cite this

Function of the nuclear receptor FTZ-F1 during the pupal stage in Drosophila melanogaster. / Sultan, Abdel Rahman S; Oish, Yasuhiro; Ueda, Hitoshi.

In: Development Growth and Differentiation, Vol. 56, No. 3, 2014, p. 245-253.

Research output: Contribution to journalArticle

@article{6ac5878022d14a0684b189aa07a23298,
title = "Function of the nuclear receptor FTZ-F1 during the pupal stage in Drosophila melanogaster",
abstract = "The nuclear receptor βFTZ-F1 is expressed in most cells in a temporally specific manner, and its expression is induced immediately after decline in ecdysteroid levels. This factor plays important roles during embryogenesis, larval ecdysis, and early metamorphic stages. However, little is known about the expression pattern, regulation and function of this receptor during the pupal stage. We analyzed the expression pattern and regulation of ftz-f1 during the pupal period, as well as the phenotypes of RNAi knockdown or mutant animals, to elucidate its function during this stage. Western blotting revealed that βFTZ-F1 is expressed at a high level during the late pupal stage, and this expression is dependent on decreasing ecdysteroid levels. By immunohistological analysis of the late pupal stage, FTZ-F1 was detected in the nuclei of most cells, but cytoplasmic localization was observed only in the oogonia and follicle cells of the ovary. Both the ftz-f1 genetic mutant and temporally specific ftz-f1 knockdown using RNAi during the pupal stage showed defects in eclosion and in the eye, the antennal segment, the wing and the leg, including bristle color and sclerosis. These results suggest that βFTZ-F1 is expressed in most cells at the late pupal stage, under the control of ecdysteroids and plays important roles during pupal development.",
keywords = "Ecdysteroid, Metamorphosis, Nuclear receptor, Pupal development, βFTZ-F1",
author = "Sultan, {Abdel Rahman S} and Yasuhiro Oish and Hitoshi Ueda",
year = "2014",
doi = "10.1111/dgd.12125",
language = "English",
volume = "56",
pages = "245--253",
journal = "Development Growth and Differentiation",
issn = "0012-1592",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Function of the nuclear receptor FTZ-F1 during the pupal stage in Drosophila melanogaster

AU - Sultan, Abdel Rahman S

AU - Oish, Yasuhiro

AU - Ueda, Hitoshi

PY - 2014

Y1 - 2014

N2 - The nuclear receptor βFTZ-F1 is expressed in most cells in a temporally specific manner, and its expression is induced immediately after decline in ecdysteroid levels. This factor plays important roles during embryogenesis, larval ecdysis, and early metamorphic stages. However, little is known about the expression pattern, regulation and function of this receptor during the pupal stage. We analyzed the expression pattern and regulation of ftz-f1 during the pupal period, as well as the phenotypes of RNAi knockdown or mutant animals, to elucidate its function during this stage. Western blotting revealed that βFTZ-F1 is expressed at a high level during the late pupal stage, and this expression is dependent on decreasing ecdysteroid levels. By immunohistological analysis of the late pupal stage, FTZ-F1 was detected in the nuclei of most cells, but cytoplasmic localization was observed only in the oogonia and follicle cells of the ovary. Both the ftz-f1 genetic mutant and temporally specific ftz-f1 knockdown using RNAi during the pupal stage showed defects in eclosion and in the eye, the antennal segment, the wing and the leg, including bristle color and sclerosis. These results suggest that βFTZ-F1 is expressed in most cells at the late pupal stage, under the control of ecdysteroids and plays important roles during pupal development.

AB - The nuclear receptor βFTZ-F1 is expressed in most cells in a temporally specific manner, and its expression is induced immediately after decline in ecdysteroid levels. This factor plays important roles during embryogenesis, larval ecdysis, and early metamorphic stages. However, little is known about the expression pattern, regulation and function of this receptor during the pupal stage. We analyzed the expression pattern and regulation of ftz-f1 during the pupal period, as well as the phenotypes of RNAi knockdown or mutant animals, to elucidate its function during this stage. Western blotting revealed that βFTZ-F1 is expressed at a high level during the late pupal stage, and this expression is dependent on decreasing ecdysteroid levels. By immunohistological analysis of the late pupal stage, FTZ-F1 was detected in the nuclei of most cells, but cytoplasmic localization was observed only in the oogonia and follicle cells of the ovary. Both the ftz-f1 genetic mutant and temporally specific ftz-f1 knockdown using RNAi during the pupal stage showed defects in eclosion and in the eye, the antennal segment, the wing and the leg, including bristle color and sclerosis. These results suggest that βFTZ-F1 is expressed in most cells at the late pupal stage, under the control of ecdysteroids and plays important roles during pupal development.

KW - Ecdysteroid

KW - Metamorphosis

KW - Nuclear receptor

KW - Pupal development

KW - βFTZ-F1

UR - http://www.scopus.com/inward/record.url?scp=84898056485&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84898056485&partnerID=8YFLogxK

U2 - 10.1111/dgd.12125

DO - 10.1111/dgd.12125

M3 - Article

C2 - 24611773

AN - SCOPUS:84898056485

VL - 56

SP - 245

EP - 253

JO - Development Growth and Differentiation

JF - Development Growth and Differentiation

SN - 0012-1592

IS - 3

ER -