Function of the Chlamydomonas reinhardtii petD 5′ untranslated region in regulating the accumulation of subunit IV of the cytochrome b6/f complex

Wataru Sakamoto, Xuemei Chen, Karen L. Kindle, David B. Stern

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Translational control is an important regulatory mechanism in chloroplasts, and is thought to be mediated by cis-acting elements in the 5′ untranslated regions (UTRs) of mRNAs. Chloroplast transformation was used to replace the wild-type Chlamydomonas reinhardtii petD 5′ UTR with mutated versions. Transformants containing altered 5′ UTRs had either a wild-type photosynthetic phenotype, a leaky non-photosynthetic phenotype, or were unable to grow photosynthetically. Among those transformants with a wild-type phenotype were ones containing mutations in a putative Shine-Dalgarno sequence element. The results indicate that two regions of the 362 nucleotide (nt) 5′ UTR may act as positive elements for translation, one located between nt 150 and 200, and the other situated approximately 40 nt upstream of the start codon, at nt 320. In every case where translation was compromised, petD mRNA accumulated to a lower level than in wild-type cells, ranging from 15% to 60% in different strains. It was concluded that specific regions of the petD 5′ UTR mediate translational activation, and that mRNA stability may be linked to translatability.

Original languageEnglish
Pages (from-to)503-512
Number of pages10
JournalPlant Journal
Volume6
Issue number4
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Cytochrome b6f Complex
Chlamydomonas reinhardtii
5' Untranslated Regions
5' untranslated regions
cytochromes
Nucleotides
nucleotides
Chloroplasts
Phenotype
phenotype
translation (genetics)
chloroplasts
Messenger RNA
start codon
Initiator Codon
RNA Stability
mutation
Mutation

ASJC Scopus subject areas

  • Plant Science

Cite this

Function of the Chlamydomonas reinhardtii petD 5′ untranslated region in regulating the accumulation of subunit IV of the cytochrome b6/f complex. / Sakamoto, Wataru; Chen, Xuemei; Kindle, Karen L.; Stern, David B.

In: Plant Journal, Vol. 6, No. 4, 1994, p. 503-512.

Research output: Contribution to journalArticle

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