FTIR Microspectroscopic Analysis of the Water Oxidation Reaction in a Single Photosystem II Microcrystal

Yuki Kato, Satoshi Haniu, Yoshiki Nakajima, Fusamichi Akita, Jian Ren Shen, Takumi Noguchi

Research output: Contribution to journalArticle

Abstract

Microcrystals of photosystem II (PSII) have recently been used to investigate the intermediate structures of the water oxidizing complex during water oxidation by serial femtosecond crystallography using X-ray free electron lasers. To clarify the water oxidation mechanism, it is crucial to know whether the reaction proceeds properly in the microcrystals. In this work, we monitored the water oxidation reaction in a single PSII microcrystal using Fourier transform infrared (FTIR) microspectroscopy with the transmission method. Flash-induced micro-FTIR difference spectra of S-state transitions in a PSII microcrystal showed features virtually identical to the corresponding spectra previously obtained using the attenuated total reflection method for multiple microcrystals, representing the reactions near the crystal surface, as well as the spectra in solution. This observation indicates that the reaction processes of water oxidation proceed with relatively high efficiencies retaining native intermediate structures in the entire inside of a PSII microcrystal.

Original languageEnglish
JournalJournal of Physical Chemistry B
DOIs
Publication statusAccepted/In press - Jan 1 2020

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Microcrystals
Photosystem II Protein Complex
microcrystals
Fourier transforms
Infrared radiation
Oxidation
oxidation
Water
water
X ray lasers
Crystallography
Free electron lasers
retaining
crystal surfaces
free electron lasers
crystallography
flash
Crystals
x rays

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

FTIR Microspectroscopic Analysis of the Water Oxidation Reaction in a Single Photosystem II Microcrystal. / Kato, Yuki; Haniu, Satoshi; Nakajima, Yoshiki; Akita, Fusamichi; Shen, Jian Ren; Noguchi, Takumi.

In: Journal of Physical Chemistry B, 01.01.2020.

Research output: Contribution to journalArticle

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