Abstract
In order to detect the precise location and pathways of calcium in the ameloblastic layer with EM and EDX, frozen ultrathin-sections, potassium pyroantimonate (PPA) method and 45Ca-autoradiography (A.R) were employed. A population of dense granules containing Ca and P was mainly observed within the nuclei and mitochondria of the frozen-sectioned ameloblasts, whereas PPA-reaction was seen not only within them but in ER, Golgi vesicles and intercellular spaces. No granules were found at the enamel matrix by both methods, but peaks for Ca and P were distinct. Silver grains of 45Ca-A.R were located in nuclei, mitochondria. Golgi complex and intercellular spaces. These results indicate that calcium can transport across the ameloblastic layer through two main pathways, extracellular and transcellular.
| Original language | English |
|---|---|
| Pages (from-to) | 1016-1018 |
| Number of pages | 3 |
| Journal | Journal of Dental Research |
| Volume | 58 |
| Issue number | Spec Issue B |
| Publication status | Published - Mar 1979 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Dentistry(all)
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Frozen ultrathin-sections for x-ray microanalysis of rat tooth germs. / Ozawa, H.; Yamamoto, T.; Yamada, M.; Uchida, T.
In: Journal of Dental Research, Vol. 58, No. Spec Issue B, 03.1979, p. 1016-1018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Frozen ultrathin-sections for x-ray microanalysis of rat tooth germs.
AU - Ozawa, H.
AU - Yamamoto, T.
AU - Yamada, M.
AU - Uchida, T.
PY - 1979/3
Y1 - 1979/3
N2 - In order to detect the precise location and pathways of calcium in the ameloblastic layer with EM and EDX, frozen ultrathin-sections, potassium pyroantimonate (PPA) method and 45Ca-autoradiography (A.R) were employed. A population of dense granules containing Ca and P was mainly observed within the nuclei and mitochondria of the frozen-sectioned ameloblasts, whereas PPA-reaction was seen not only within them but in ER, Golgi vesicles and intercellular spaces. No granules were found at the enamel matrix by both methods, but peaks for Ca and P were distinct. Silver grains of 45Ca-A.R were located in nuclei, mitochondria. Golgi complex and intercellular spaces. These results indicate that calcium can transport across the ameloblastic layer through two main pathways, extracellular and transcellular.
AB - In order to detect the precise location and pathways of calcium in the ameloblastic layer with EM and EDX, frozen ultrathin-sections, potassium pyroantimonate (PPA) method and 45Ca-autoradiography (A.R) were employed. A population of dense granules containing Ca and P was mainly observed within the nuclei and mitochondria of the frozen-sectioned ameloblasts, whereas PPA-reaction was seen not only within them but in ER, Golgi vesicles and intercellular spaces. No granules were found at the enamel matrix by both methods, but peaks for Ca and P were distinct. Silver grains of 45Ca-A.R were located in nuclei, mitochondria. Golgi complex and intercellular spaces. These results indicate that calcium can transport across the ameloblastic layer through two main pathways, extracellular and transcellular.
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M3 - Article
C2 - 283105
AN - SCOPUS:0018442534
VL - 58
SP - 1016
EP - 1018
JO - Journal of Dental Research
JF - Journal of Dental Research
SN - 0022-0345
IS - Spec Issue B
ER -